Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano
The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcrip...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/20607 |
| _version_ | 1811200207815180288 |
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| author | Magda Grudniewska Stijn Mouton Daniil Simanov Frank Beltman Margriet Grelling Katrien de Mulder Wibowo Arindrarto Philipp M. Weissert Stefan van der Elst Eugene Berezikov |
| author_facet | Magda Grudniewska Stijn Mouton Daniil Simanov Frank Beltman Margriet Grelling Katrien de Mulder Wibowo Arindrarto Philipp M. Weissert Stefan van der Elst Eugene Berezikov |
| author_sort | Magda Grudniewska |
| collection | DOAJ |
| description | The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano. |
| first_indexed | 2024-04-12T01:59:47Z |
| format | Article |
| id | doaj.art-ec4a9467c77a4ce0b0e7e70f4fb049d3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:59:47Z |
| publishDate | 2016-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ec4a9467c77a4ce0b0e7e70f4fb049d32022-12-22T03:52:42ZengeLife Sciences Publications LtdeLife2050-084X2016-12-01510.7554/eLife.20607Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignanoMagda Grudniewska0Stijn Mouton1Daniil Simanov2Frank Beltman3Margriet Grelling4Katrien de Mulder5Wibowo Arindrarto6Philipp M. Weissert7Stefan van der Elst8Eugene Berezikov9https://orcid.org/0000-0002-1145-2884European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Hubrecht Institute-KNAW, Utrecht, The Netherlands; University Medical Centre Utrecht, Utrecht, The NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Hubrecht Institute-KNAW, Utrecht, The Netherlands; University Medical Centre Utrecht, Utrecht, The NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The NetherlandsHubrecht Institute-KNAW, Utrecht, The Netherlands; University Medical Centre Utrecht, Utrecht, The NetherlandsHubrecht Institute-KNAW, Utrecht, The Netherlands; University Medical Centre Utrecht, Utrecht, The NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The NetherlandsHubrecht Institute-KNAW, Utrecht, The Netherlands; University Medical Centre Utrecht, Utrecht, The NetherlandsEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands; Hubrecht Institute-KNAW, Utrecht, The Netherlands; University Medical Centre Utrecht, Utrecht, The NetherlandsThe regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.https://elifesciences.org/articles/20607flatwormsMacrostomumstem cellsregenerationgermline |
| spellingShingle | Magda Grudniewska Stijn Mouton Daniil Simanov Frank Beltman Margriet Grelling Katrien de Mulder Wibowo Arindrarto Philipp M. Weissert Stefan van der Elst Eugene Berezikov Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano eLife flatworms Macrostomum stem cells regeneration germline |
| title | Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano |
| title_full | Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano |
| title_fullStr | Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano |
| title_full_unstemmed | Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano |
| title_short | Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano |
| title_sort | transcriptional signatures of somatic neoblasts and germline cells in macrostomum lignano |
| topic | flatworms Macrostomum stem cells regeneration germline |
| url | https://elifesciences.org/articles/20607 |
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