Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification
<p>Several micro-scale chromatography-based procedures for purification of the &bgr;-galactosidase from the yeast <it>Kluyveromyces lactis</it> were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
1998-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/4/m4.htm |
| Summary: | <p>Several micro-scale chromatography-based procedures for purification of the &bgr;-galactosidase from the yeast <it>Kluyveromyces lactis</it> were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme. |
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| ISSN: | 1480-9222 |