Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification
<p>Several micro-scale chromatography-based procedures for purification of the &bgr;-galactosidase from the yeast <it>Kluyveromyces lactis</it> were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
1998-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/4/m4.htm |
| _version_ | 1818230188894322688 |
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| author | Becerra M Cerdãn E Gonzãlez Siso MI |
| author_facet | Becerra M Cerdãn E Gonzãlez Siso MI |
| author_sort | Becerra M |
| collection | DOAJ |
| description | <p>Several micro-scale chromatography-based procedures for purification of the &bgr;-galactosidase from the yeast <it>Kluyveromyces lactis</it> were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme. |
| first_indexed | 2024-12-12T10:30:32Z |
| format | Article |
| id | doaj.art-ec4b87fa38f84fb78a3e9c1c51769913 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-12T10:30:32Z |
| publishDate | 1998-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-ec4b87fa38f84fb78a3e9c1c517699132022-12-22T00:27:23ZengBMCBiological Procedures Online1480-92221998-01-0111485810.1251/bpo4Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purificationBecerra MCerdãn EGonzãlez Siso MI<p>Several micro-scale chromatography-based procedures for purification of the &bgr;-galactosidase from the yeast <it>Kluyveromyces lactis</it> were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.http://www.biologicalprocedures.com/bpo/arts/1/4/m4.htmKluyveromycesGlucosidases |
| spellingShingle | Becerra M Cerdãn E Gonzãlez Siso MI Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification Biological Procedures Online Kluyveromyces Glucosidases |
| title | Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification |
| title_full | Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification |
| title_fullStr | Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification |
| title_full_unstemmed | Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification |
| title_short | Dealing with different methods for <it>Kluyveromyces lactis</it> &bgr;-galactosidase purification |
| title_sort | dealing with different methods for it kluyveromyces lactis it bgr galactosidase purification |
| topic | Kluyveromyces Glucosidases |
| url | http://www.biologicalprocedures.com/bpo/arts/1/4/m4.htm |
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