Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder
Transitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal k...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2010-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013 |
| _version_ | 1818765368036950016 |
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| author | Marija Pljesa-Ercegovac Ana Savic-Radojevic Tamara Kravic-Stevovic Vladimir Bumbasirevic Jasmina Mimic-Oka Tatiana Simic |
| author_facet | Marija Pljesa-Ercegovac Ana Savic-Radojevic Tamara Kravic-Stevovic Vladimir Bumbasirevic Jasmina Mimic-Oka Tatiana Simic |
| author_sort | Marija Pljesa-Ercegovac |
| collection | DOAJ |
| description | Transitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC samples studied. A co-localization of GSTP1 and JNK was also demonstrated in the 5637 TCC cell line by means of confocal microscopy. Protein-protein interactions, together with co-localization between GSTP1 and JNK provide evidence that GSTP1 most probably inhibits apoptosis in TCC cells by non-covalent binding to JNK. |
| first_indexed | 2024-12-18T08:16:59Z |
| format | Article |
| id | doaj.art-ec4ecd79eb774770b6d5c647a3fe8edf |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-18T08:16:59Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-ec4ecd79eb774770b6d5c647a3fe8edf2022-12-21T21:14:48ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852010-01-01333460462Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladderMarija Pljesa-ErcegovacAna Savic-RadojevicTamara Kravic-StevovicVladimir BumbasirevicJasmina Mimic-OkaTatiana SimicTransitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC samples studied. A co-localization of GSTP1 and JNK was also demonstrated in the 5637 TCC cell line by means of confocal microscopy. Protein-protein interactions, together with co-localization between GSTP1 and JNK provide evidence that GSTP1 most probably inhibits apoptosis in TCC cells by non-covalent binding to JNK.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013glutathione S-transferaseJNKTCCco-localization |
| spellingShingle | Marija Pljesa-Ercegovac Ana Savic-Radojevic Tamara Kravic-Stevovic Vladimir Bumbasirevic Jasmina Mimic-Oka Tatiana Simic Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder Genetics and Molecular Biology glutathione S-transferase JNK TCC co-localization |
| title | Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder |
| title_full | Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder |
| title_fullStr | Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder |
| title_full_unstemmed | Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder |
| title_short | Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder |
| title_sort | co localization of gstp1 and jnk in transitional cell carcinoma of urinary bladder |
| topic | glutathione S-transferase JNK TCC co-localization |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013 |
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