Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat
Available data showed that the recovery of multidrug-resistant and biofilm-producing Salmonella spp. from chicken meat is still scanty in Nigeria. Consequently, this study aimed to characterize the probable multidrugresistant and biofilm producing Salmonella spp. prevalent in chicken meat vended in...
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| Format: | Article |
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The National Information and Documentation Centre (NIDOC) , Egypt
2021-04-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_164551_3ea30c009664efaeb6b390d218778bb8.pdf |
| _version_ | 1797204362989142016 |
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| author | G.I. Ogu J.C. Okolo J.C. Igborgbor F.I. Akinnibosun |
| author_facet | G.I. Ogu J.C. Okolo J.C. Igborgbor F.I. Akinnibosun |
| author_sort | G.I. Ogu |
| collection | DOAJ |
| description | Available data showed that the recovery of multidrug-resistant and biofilm-producing Salmonella spp. from
chicken meat is still scanty in Nigeria. Consequently, this study aimed to characterize the probable multidrugresistant and biofilm producing Salmonella spp. prevalent in chicken meat vended in southern Nigerian markets.
About 240 randomly sampled chilled raw chicken meats were collected from open markets in Delta, Edo, Ekiti
and Ondo States, and then were analysed for detecting the presence of Salmonella spp.; using rinse
centrifugation-plating technique, serological examination and 16S rRNA gene sequencing. The confirmed
Salmonella isolates were tested for multidrug-resistance and biofilm formation using Kirby Bauer disc diffusion
test and tissue culture plates, respectively. Out of 229 presumptively examined Salmonella isolates, 52 isolates
were confirmed as Salmonella spp., while 46 isolates were recorded as multidrug-resistant. The main serotypes
recovered were; S. enterica subsp. enterica serovar Typhimurium (35/52; 67.31%), and S. enterica subsp. enterica
serovar Enteritidis (17/52; 32.69%). Biofilm characterization of the recovered Salmonella isolates were; strong
(OD > 0.240), 6 (11.5 %); moderate (0.120-0.240), 13 (25.0 %); weak (OD < 0.120), 19 (36.5 %), and nonbiofilm producers (OD < 0.120), 14 (26.9 %). This study showed that multidrug-resistant and biofilm-producing
Salmonella spp. were prevalent in raw chicken meat; vended within southern Nigerian open markets. Thus, there
is an urgent need for relevant regulatory agencies to enforce consumer's safety. |
| first_indexed | 2024-04-13T07:22:59Z |
| format | Article |
| id | doaj.art-ec54565cb815442395dff7c35f83d260 |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:34:02Z |
| publishDate | 2021-04-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-ec54565cb815442395dff7c35f83d2602024-04-16T18:18:42ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942021-04-01521214122610.21608/nrmj.2021.164551Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meatG.I. Ogu0J.C. Okolo1J.C. Igborgbor2F.I. Akinnibosun3Department of Microbiology, Federal University Lokoja, Lokoja, Kogi State, NigeriaDepartment of Environmental Biotechnology and Bio-conservation, National Biotechnology Development Agency, Abuja, NigeriaDepartment of Biology, University of Delta, Abgor, Delta State, NigeriaDepartment of Microbiology, University of Benin, Benin City, Edo State, NigeriaAvailable data showed that the recovery of multidrug-resistant and biofilm-producing Salmonella spp. from chicken meat is still scanty in Nigeria. Consequently, this study aimed to characterize the probable multidrugresistant and biofilm producing Salmonella spp. prevalent in chicken meat vended in southern Nigerian markets. About 240 randomly sampled chilled raw chicken meats were collected from open markets in Delta, Edo, Ekiti and Ondo States, and then were analysed for detecting the presence of Salmonella spp.; using rinse centrifugation-plating technique, serological examination and 16S rRNA gene sequencing. The confirmed Salmonella isolates were tested for multidrug-resistance and biofilm formation using Kirby Bauer disc diffusion test and tissue culture plates, respectively. Out of 229 presumptively examined Salmonella isolates, 52 isolates were confirmed as Salmonella spp., while 46 isolates were recorded as multidrug-resistant. The main serotypes recovered were; S. enterica subsp. enterica serovar Typhimurium (35/52; 67.31%), and S. enterica subsp. enterica serovar Enteritidis (17/52; 32.69%). Biofilm characterization of the recovered Salmonella isolates were; strong (OD > 0.240), 6 (11.5 %); moderate (0.120-0.240), 13 (25.0 %); weak (OD < 0.120), 19 (36.5 %), and nonbiofilm producers (OD < 0.120), 14 (26.9 %). This study showed that multidrug-resistant and biofilm-producing Salmonella spp. were prevalent in raw chicken meat; vended within southern Nigerian open markets. Thus, there is an urgent need for relevant regulatory agencies to enforce consumer's safety.https://nrmj.journals.ekb.eg/article_164551_3ea30c009664efaeb6b390d218778bb8.pdfbiofilmmultidrug-resistant salmonellachicken meattissue culture plates |
| spellingShingle | G.I. Ogu J.C. Okolo J.C. Igborgbor F.I. Akinnibosun Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat Novel Research in Microbiology Journal biofilm multidrug-resistant salmonella chicken meat tissue culture plates |
| title | Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat |
| title_full | Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat |
| title_fullStr | Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat |
| title_full_unstemmed | Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat |
| title_short | Multidrug-resistance and biofilm formation profiles of Salmonella spp. isolated from raw chicken meat |
| title_sort | multidrug resistance and biofilm formation profiles of salmonella spp isolated from raw chicken meat |
| topic | biofilm multidrug-resistant salmonella chicken meat tissue culture plates |
| url | https://nrmj.journals.ekb.eg/article_164551_3ea30c009664efaeb6b390d218778bb8.pdf |
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