A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system
Marburg virus (MARV) can cause lethal hemorrhagic fever in humans. Handling of MARV is restricted to high-containment biosafety level 4 (BSL-4) facilities, which greatly impedes research into this virus. In this study, a high titer of MARV pseudovirus was generated through optimization of the HIV ba...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-08-01
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| Series: | Human Vaccines & Immunotherapeutics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21645515.2017.1325050 |
| _version_ | 1797677582928314368 |
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| author | Li Zhang Qianqian Li Qiang Liu Weijin Huang Jianhui Nie Youchun Wang |
| author_facet | Li Zhang Qianqian Li Qiang Liu Weijin Huang Jianhui Nie Youchun Wang |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | Marburg virus (MARV) can cause lethal hemorrhagic fever in humans. Handling of MARV is restricted to high-containment biosafety level 4 (BSL-4) facilities, which greatly impedes research into this virus. In this study, a high titer of MARV pseudovirus was generated through optimization of the HIV backbone vectors, the ratio of backbone vector to MARV glycoprotein expression vector, and the transfection reagents. An in vitro neutralization assay and an in vivo bioluminescent imaging mouse model for MARV were developed based on the pseudovirus. Protective serum against MARV was successfully induced in guinea pigs, which showed high neutralization activity in vitro and could also protect Balb/c mice from MARV pseudovirus infection in vivo. This system could be a convenient tool to enable the evaluation of vaccines and therapeutic drugs against MARV in non-BSL-4 laboratories. |
| first_indexed | 2024-03-11T22:47:21Z |
| format | Article |
| id | doaj.art-ec551b64d036402f948ecc382db73bc9 |
| institution | Directory Open Access Journal |
| issn | 2164-5515 2164-554X |
| language | English |
| last_indexed | 2024-03-11T22:47:21Z |
| publishDate | 2017-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj.art-ec551b64d036402f948ecc382db73bc92023-09-22T08:17:50ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2017-08-011381811181710.1080/21645515.2017.13250501325050A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus systemLi Zhang0Qianqian Li1Qiang Liu2Weijin Huang3Jianhui Nie4Youchun Wang5National Institutes for Food and Drug ControlNational Institutes for Food and Drug ControlNational Institutes for Food and Drug ControlNational Institutes for Food and Drug ControlNational Institutes for Food and Drug ControlNational Institutes for Food and Drug ControlMarburg virus (MARV) can cause lethal hemorrhagic fever in humans. Handling of MARV is restricted to high-containment biosafety level 4 (BSL-4) facilities, which greatly impedes research into this virus. In this study, a high titer of MARV pseudovirus was generated through optimization of the HIV backbone vectors, the ratio of backbone vector to MARV glycoprotein expression vector, and the transfection reagents. An in vitro neutralization assay and an in vivo bioluminescent imaging mouse model for MARV were developed based on the pseudovirus. Protective serum against MARV was successfully induced in guinea pigs, which showed high neutralization activity in vitro and could also protect Balb/c mice from MARV pseudovirus infection in vivo. This system could be a convenient tool to enable the evaluation of vaccines and therapeutic drugs against MARV in non-BSL-4 laboratories.http://dx.doi.org/10.1080/21645515.2017.1325050animal modelbioluminescent imagingmarburg viruspassive protectionpseudovirus |
| spellingShingle | Li Zhang Qianqian Li Qiang Liu Weijin Huang Jianhui Nie Youchun Wang A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system Human Vaccines & Immunotherapeutics animal model bioluminescent imaging marburg virus passive protection pseudovirus |
| title | A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system |
| title_full | A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system |
| title_fullStr | A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system |
| title_full_unstemmed | A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system |
| title_short | A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system |
| title_sort | bioluminescent imaging mouse model for marburg virus based on a pseudovirus system |
| topic | animal model bioluminescent imaging marburg virus passive protection pseudovirus |
| url | http://dx.doi.org/10.1080/21645515.2017.1325050 |
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