Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it>
<p>Abstract</p> <p><it>Clostridium chauvoei </it>is the causative agent of blackleg, a wide spread serious infection of cattle and sheep with high mortality. In this study we have analyzed the sialidase activity of the NanA protein of <it>C. chauvoei </it>an...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-01-01
|
| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/42/1/2 |
| _version_ | 1818787036543320064 |
|---|---|
| author | Vilei Edy M Johansson Anders Schlatter Yvonne Redhead Keith Frey Joachim |
| author_facet | Vilei Edy M Johansson Anders Schlatter Yvonne Redhead Keith Frey Joachim |
| author_sort | Vilei Edy M |
| collection | DOAJ |
| description | <p>Abstract</p> <p><it>Clostridium chauvoei </it>is the causative agent of blackleg, a wide spread serious infection of cattle and sheep with high mortality. In this study we have analyzed the sialidase activity of the NanA protein of <it>C. chauvoei </it>and cloned the sialidase gene <it>nanA</it>. Sialidase is encoded as a precursor protein of 722 amino acids with a 26 amino acid signal peptide. The mature sialidase has a calculated molecular mass of 81 kDa and contains the carbohydrate binding module 32 (CBM32, or F5/8 type C domain), the sialic acid binding module CBM40 and the enzymatically active sialidase domain found in all pro- and eukaryotic sialidases. Sialidase activity does not require the CBM32 domain. The NanA protein is secreted by <it>C. chauvoei </it>as a dimer. The <it>nanA </it>gene was found to be conserved and sialidase activity was found in <it>C. chauvoei </it>strains isolated over a period of 50 years from various geographical locations. Antiserum directed against a recombinant 40 kDa peptide containing CBM40 and part of the enzymatically active domain of NanA neutralized the secreted sialidase activity of all <it>C. chauvoei </it>strains tested.</p> |
| first_indexed | 2024-12-18T14:01:23Z |
| format | Article |
| id | doaj.art-ec567119fe2a4ee19fd59910d1b57582 |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-12-18T14:01:23Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-ec567119fe2a4ee19fd59910d1b575822022-12-21T21:05:20ZengBMCVeterinary Research0928-42491297-97162011-01-01421210.1186/1297-9716-42-2Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it>Vilei Edy MJohansson AndersSchlatter YvonneRedhead KeithFrey Joachim<p>Abstract</p> <p><it>Clostridium chauvoei </it>is the causative agent of blackleg, a wide spread serious infection of cattle and sheep with high mortality. In this study we have analyzed the sialidase activity of the NanA protein of <it>C. chauvoei </it>and cloned the sialidase gene <it>nanA</it>. Sialidase is encoded as a precursor protein of 722 amino acids with a 26 amino acid signal peptide. The mature sialidase has a calculated molecular mass of 81 kDa and contains the carbohydrate binding module 32 (CBM32, or F5/8 type C domain), the sialic acid binding module CBM40 and the enzymatically active sialidase domain found in all pro- and eukaryotic sialidases. Sialidase activity does not require the CBM32 domain. The NanA protein is secreted by <it>C. chauvoei </it>as a dimer. The <it>nanA </it>gene was found to be conserved and sialidase activity was found in <it>C. chauvoei </it>strains isolated over a period of 50 years from various geographical locations. Antiserum directed against a recombinant 40 kDa peptide containing CBM40 and part of the enzymatically active domain of NanA neutralized the secreted sialidase activity of all <it>C. chauvoei </it>strains tested.</p>http://www.veterinaryresearch.org/content/42/1/2 |
| spellingShingle | Vilei Edy M Johansson Anders Schlatter Yvonne Redhead Keith Frey Joachim Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it> Veterinary Research |
| title | Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it> |
| title_full | Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it> |
| title_fullStr | Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it> |
| title_full_unstemmed | Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it> |
| title_short | Genetic and functional characterization of the NanA sialidase from <it>Clostridium chauvoei</it> |
| title_sort | genetic and functional characterization of the nana sialidase from it clostridium chauvoei it |
| url | http://www.veterinaryresearch.org/content/42/1/2 |
| work_keys_str_mv | AT vileiedym geneticandfunctionalcharacterizationofthenanasialidasefromitclostridiumchauvoeiit AT johanssonanders geneticandfunctionalcharacterizationofthenanasialidasefromitclostridiumchauvoeiit AT schlatteryvonne geneticandfunctionalcharacterizationofthenanasialidasefromitclostridiumchauvoeiit AT redheadkeith geneticandfunctionalcharacterizationofthenanasialidasefromitclostridiumchauvoeiit AT freyjoachim geneticandfunctionalcharacterizationofthenanasialidasefromitclostridiumchauvoeiit |