A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds
A protein of an apparent molecular mass of 14.4 kDa with antifungal activity was isolated from the seeds of Pithecellobium dulce using extraction with 100 mM Tris-HCl buffer (pH=8.0), precipitation with 80 % ammonium sulfate, and bioassay purification via Resource Q anion exchange chromatography and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2011-01-01
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| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/111619 |
| Summary: | A protein of an apparent molecular mass of 14.4 kDa with antifungal activity was isolated from the seeds of Pithecellobium dulce using extraction with 100 mM Tris-HCl buffer (pH=8.0), precipitation with 80 % ammonium sulfate, and bioassay purification via Resource Q anion exchange chromatography and Superdex 200 gel filtration chromatography. The purified protein was putatively identified by tandem mass spectrometry with Mascot database searching, with the partial amino acid sequences showing a high degree of similarity to chicken egg white lysozyme. This putative plant lysozyme expressed antifungal activity with a rather high thermal stability of up to 80 °C for 15 min (at pH=8.0). It exerted an antifungal action towards Macrophomina phaseolina but displayed no antifungal activity against two other isolates, Phymatotrichopsis omnivora and Fusarium avenaceum. |
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| ISSN: | 1330-9862 1334-2606 |