A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds
A protein of an apparent molecular mass of 14.4 kDa with antifungal activity was isolated from the seeds of Pithecellobium dulce using extraction with 100 mM Tris-HCl buffer (pH=8.0), precipitation with 80 % ammonium sulfate, and bioassay purification via Resource Q anion exchange chromatography and...
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University of Zagreb Faculty of Food Technology and Biotechnology
2011-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/111619 |
| _version_ | 1797428350526947328 |
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| author | Ploypat Niyomploy Lloyd W. Sumner Zhentian Lei Narumon Sawasdipuksa Polkit Sangvanich |
| author_facet | Ploypat Niyomploy Lloyd W. Sumner Zhentian Lei Narumon Sawasdipuksa Polkit Sangvanich |
| author_sort | Ploypat Niyomploy |
| collection | DOAJ |
| description | A protein of an apparent molecular mass of 14.4 kDa with antifungal activity was isolated from the seeds of Pithecellobium dulce using extraction with 100 mM Tris-HCl buffer (pH=8.0), precipitation with 80 % ammonium sulfate, and bioassay purification via Resource Q anion exchange chromatography and Superdex 200 gel filtration chromatography. The purified protein was putatively identified by tandem mass spectrometry with Mascot database searching, with the partial amino acid sequences showing a high degree of similarity to chicken egg white lysozyme. This putative plant lysozyme expressed antifungal activity with a rather high thermal stability of up to 80 °C for 15 min (at pH=8.0). It exerted an antifungal action towards Macrophomina phaseolina but displayed no antifungal activity against two other isolates, Phymatotrichopsis omnivora and Fusarium avenaceum. |
| first_indexed | 2024-03-09T08:56:41Z |
| format | Article |
| id | doaj.art-ec579b7c1e894e91891eafc8bd6359c5 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:56:41Z |
| publishDate | 2011-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-ec579b7c1e894e91891eafc8bd6359c52023-12-02T12:57:16ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062011-01-01494489494A Lysozyme with Antifungal Activity from Pithecellobium dulce SeedsPloypat Niyomploy0Lloyd W. Sumner1Zhentian Lei2Narumon Sawasdipuksa3Polkit Sangvanich4Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandPlant Biology Division, The Samuel Roberts Noble Foundation, Ardmore 73401, Oklahoma, USAPlant Biology Division, The Samuel Roberts Noble Foundation, Ardmore 73401, Oklahoma, USAResearch Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandResearch Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandA protein of an apparent molecular mass of 14.4 kDa with antifungal activity was isolated from the seeds of Pithecellobium dulce using extraction with 100 mM Tris-HCl buffer (pH=8.0), precipitation with 80 % ammonium sulfate, and bioassay purification via Resource Q anion exchange chromatography and Superdex 200 gel filtration chromatography. The purified protein was putatively identified by tandem mass spectrometry with Mascot database searching, with the partial amino acid sequences showing a high degree of similarity to chicken egg white lysozyme. This putative plant lysozyme expressed antifungal activity with a rather high thermal stability of up to 80 °C for 15 min (at pH=8.0). It exerted an antifungal action towards Macrophomina phaseolina but displayed no antifungal activity against two other isolates, Phymatotrichopsis omnivora and Fusarium avenaceum.http://hrcak.srce.hr/file/111619Pithecellobium dulceseed proteinlysozymeantifungal activity |
| spellingShingle | Ploypat Niyomploy Lloyd W. Sumner Zhentian Lei Narumon Sawasdipuksa Polkit Sangvanich A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds Food Technology and Biotechnology Pithecellobium dulce seed protein lysozyme antifungal activity |
| title | A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds |
| title_full | A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds |
| title_fullStr | A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds |
| title_full_unstemmed | A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds |
| title_short | A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds |
| title_sort | lysozyme with antifungal activity from pithecellobium dulce seeds |
| topic | Pithecellobium dulce seed protein lysozyme antifungal activity |
| url | http://hrcak.srce.hr/file/111619 |
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