Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) Pods
Malaria is an infectious and parasitic disease caused by protozoa of the genus <i>Plasmodium</i>, which affects millions of people in tropical and subtropical areas. Recently, there have been multiple reports of drug resistance in <i>Plasmodium</i> populations, leading to the...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/15/4/1162 |
| _version_ | 1827744075137155072 |
|---|---|
| author | Francisco Flávio Vieira de Assis José Sousa de Almeida Junior Tânia Mara Pires Moraes Fernando de Pilla Varotti Camila Castilho Moraes Adilson Sartoratto Waldiney Pires Moraes Antonio Humberto Hamad Minervino |
| author_facet | Francisco Flávio Vieira de Assis José Sousa de Almeida Junior Tânia Mara Pires Moraes Fernando de Pilla Varotti Camila Castilho Moraes Adilson Sartoratto Waldiney Pires Moraes Antonio Humberto Hamad Minervino |
| author_sort | Francisco Flávio Vieira de Assis |
| collection | DOAJ |
| description | Malaria is an infectious and parasitic disease caused by protozoa of the genus <i>Plasmodium</i>, which affects millions of people in tropical and subtropical areas. Recently, there have been multiple reports of drug resistance in <i>Plasmodium</i> populations, leading to the search for potential new active compounds against the parasite. Thus, we aimed to evaluate the in vitro antiplasmodial activity and cytotoxicity of the hydroalcoholic extract of Jucá (<i>Libidibia ferrea)</i> in serial concentrations. Jucá was used in the form of a freeze-dried hydroalcoholic extract. For the cytotoxicity assay, the(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method with the WI-26VA4 human cell line was used. For the antiplasmodial activity, <i>Plasmodium falciparum</i> synchronized cultures were treated with serial concentrations (0.2 to 50 μg/mL) of the Jucá extract. In terms of the chemical composition of the Jucá extract, gas chromatography coupled to mass spectrometry measurements revealed the main compounds as ellagic acid, valoneic acid dilactone, gallotannin, and gallic acid. The Jucá hydroalcoholic extract did not show cytotoxic activity per MTT, with an IC<sub>50</sub> value greater than 100 µg/mL. Regarding the antiplasmodial activity, the Jucá extract presented an IC<sub>50</sub> of 11.10 µg/mL with a selective index of nine. Because of its antiplasmodial activity at the tested concentrations and low toxicity, the Jucá extract is presented as a candidate for herbal medicine in the treatment of malaria. To the best of our knowledge, this is the first report of antiplasmodial activity in Jucá. |
| first_indexed | 2024-03-11T04:38:47Z |
| format | Article |
| id | doaj.art-ec5a71112e384f329033f9758cb6b1be |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T04:38:47Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-ec5a71112e384f329033f9758cb6b1be2023-11-17T20:53:36ZengMDPI AGPharmaceutics1999-49232023-04-01154116210.3390/pharmaceutics15041162Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) PodsFrancisco Flávio Vieira de Assis0José Sousa de Almeida Junior1Tânia Mara Pires Moraes2Fernando de Pilla Varotti3Camila Castilho Moraes4Adilson Sartoratto5Waldiney Pires Moraes6Antonio Humberto Hamad Minervino7Laboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Santarém 68040-255, BrazilLaboratório de Farmacologia Experimental, Universidade Federal do Oeste do Pará, UFOPA, Santarém 68040-255, BrazilLaboratório de Farmacologia Experimental, Universidade Federal do Oeste do Pará, UFOPA, Santarém 68040-255, BrazilNúcleo de Pesquisa em Química Biológica (NQBio), Universidade Federal de São João Del Rei, Campus Centro-Oeste, Av. Sebastião G. Coelho, 400, Chanadour, Divinópolis 35501-296, BrazilLaboratório de Farmacologia Experimental, Universidade Federal do Oeste do Pará, UFOPA, Santarém 68040-255, BrazilCentro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, Universidade de Campinas—UNICAMP, Campinas 13148-218, BrazilLaboratório de Farmacologia Experimental, Universidade Federal do Oeste do Pará, UFOPA, Santarém 68040-255, BrazilLaboratory of Animal Health, LARSANA, Federal University of Western Pará, UFOPA, Santarém 68040-255, BrazilMalaria is an infectious and parasitic disease caused by protozoa of the genus <i>Plasmodium</i>, which affects millions of people in tropical and subtropical areas. Recently, there have been multiple reports of drug resistance in <i>Plasmodium</i> populations, leading to the search for potential new active compounds against the parasite. Thus, we aimed to evaluate the in vitro antiplasmodial activity and cytotoxicity of the hydroalcoholic extract of Jucá (<i>Libidibia ferrea)</i> in serial concentrations. Jucá was used in the form of a freeze-dried hydroalcoholic extract. For the cytotoxicity assay, the(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) method with the WI-26VA4 human cell line was used. For the antiplasmodial activity, <i>Plasmodium falciparum</i> synchronized cultures were treated with serial concentrations (0.2 to 50 μg/mL) of the Jucá extract. In terms of the chemical composition of the Jucá extract, gas chromatography coupled to mass spectrometry measurements revealed the main compounds as ellagic acid, valoneic acid dilactone, gallotannin, and gallic acid. The Jucá hydroalcoholic extract did not show cytotoxic activity per MTT, with an IC<sub>50</sub> value greater than 100 µg/mL. Regarding the antiplasmodial activity, the Jucá extract presented an IC<sub>50</sub> of 11.10 µg/mL with a selective index of nine. Because of its antiplasmodial activity at the tested concentrations and low toxicity, the Jucá extract is presented as a candidate for herbal medicine in the treatment of malaria. To the best of our knowledge, this is the first report of antiplasmodial activity in Jucá.https://www.mdpi.com/1999-4923/15/4/1162malariaJucáherbal medicinesAmazon |
| spellingShingle | Francisco Flávio Vieira de Assis José Sousa de Almeida Junior Tânia Mara Pires Moraes Fernando de Pilla Varotti Camila Castilho Moraes Adilson Sartoratto Waldiney Pires Moraes Antonio Humberto Hamad Minervino Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) Pods Pharmaceutics malaria Jucá herbal medicines Amazon |
| title | Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) Pods |
| title_full | Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) Pods |
| title_fullStr | Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) Pods |
| title_full_unstemmed | Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) Pods |
| title_short | Antiplasmodial Activity of Hydroalcoholic Extract from Jucá (<i>Libidibia ferrea</i>) Pods |
| title_sort | antiplasmodial activity of hydroalcoholic extract from juca i libidibia ferrea i pods |
| topic | malaria Jucá herbal medicines Amazon |
| url | https://www.mdpi.com/1999-4923/15/4/1162 |
| work_keys_str_mv | AT franciscoflaviovieiradeassis antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods AT josesousadealmeidajunior antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods AT taniamarapiresmoraes antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods AT fernandodepillavarotti antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods AT camilacastilhomoraes antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods AT adilsonsartoratto antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods AT waldineypiresmoraes antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods AT antoniohumbertohamadminervino antiplasmodialactivityofhydroalcoholicextractfromjucailibidibiaferreaipods |