The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis.
Bacterial vaginosis (BV), a condition in which the vaginal microbiota consists of community of obligate and facultative anaerobes rather than dominated by a single species of Lactobacillus, affects ~30% of women in the US. Women with BV are at 60% increased risk for HIV acquisition and are 3-times m...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1008236 |
| _version_ | 1819045088115818496 |
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| author | Thuy Hoang Emily Toler Kevin DeLong Nomfuneko A Mafunda Seth M Bloom Hannah C Zierden Thomas R Moench Jenell S Coleman Justin Hanes Douglas S Kwon Samuel K Lai Richard A Cone Laura M Ensign |
| author_facet | Thuy Hoang Emily Toler Kevin DeLong Nomfuneko A Mafunda Seth M Bloom Hannah C Zierden Thomas R Moench Jenell S Coleman Justin Hanes Douglas S Kwon Samuel K Lai Richard A Cone Laura M Ensign |
| author_sort | Thuy Hoang |
| collection | DOAJ |
| description | Bacterial vaginosis (BV), a condition in which the vaginal microbiota consists of community of obligate and facultative anaerobes rather than dominated by a single species of Lactobacillus, affects ~30% of women in the US. Women with BV are at 60% increased risk for HIV acquisition and are 3-times more likely to transmit HIV to an uninfected partner. As cervicovaginal mucus (CVM) is the first line of defense against mucosal pathogens and the home of the resident vaginal microbiota, we hypothesized the barrier function of CVM to HIV may be diminished in BV. Here, we characterized CVM properties including pH, lactic acid content, and Nugent score to correlate with the microbiota community composition, which was confirmed by 16S rDNA sequencing on a subset of samples. We then quantified the mobility of fluorescently-labeled HIV virions and nanoparticles to characterize the structural and adhesive barrier properties of CVM. Our analyses included women with Nugent scores categorized as intermediate (4-6) and BV (7-10), women that were either symptomatic or asymptomatic, and a small group of women before and after antibiotic treatment for symptomatic BV. Overall, we found that HIV virions had significantly increased mobility in CVM from women with BV compared to CVM from women with Lactobacillus crispatus-dominant microbiota, regardless of whether symptoms were present. We confirmed using nanoparticles and scanning electron microscopy that the impaired barrier function was due to reduced adhesive barrier properties without an obvious degradation of the physical CVM pore structure. We further confirmed a similar increase in HIV mobility in CVM from women with Lactobacillus iners-dominant microbiota, the species most associated with transitions to BV and that persists after antibiotic treatment for BV. Our findings advance the understanding of the protective role of mucus and highlight the interplay between vaginal microbiota and the innate barrier function mucus. |
| first_indexed | 2024-12-21T10:23:00Z |
| format | Article |
| id | doaj.art-ec5ae9c7663a4314ad6929bbc4c39a75 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-21T10:23:00Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-ec5ae9c7663a4314ad6929bbc4c39a752022-12-21T19:07:23ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742020-01-01161e100823610.1371/journal.ppat.1008236The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis.Thuy HoangEmily TolerKevin DeLongNomfuneko A MafundaSeth M BloomHannah C ZierdenThomas R MoenchJenell S ColemanJustin HanesDouglas S KwonSamuel K LaiRichard A ConeLaura M EnsignBacterial vaginosis (BV), a condition in which the vaginal microbiota consists of community of obligate and facultative anaerobes rather than dominated by a single species of Lactobacillus, affects ~30% of women in the US. Women with BV are at 60% increased risk for HIV acquisition and are 3-times more likely to transmit HIV to an uninfected partner. As cervicovaginal mucus (CVM) is the first line of defense against mucosal pathogens and the home of the resident vaginal microbiota, we hypothesized the barrier function of CVM to HIV may be diminished in BV. Here, we characterized CVM properties including pH, lactic acid content, and Nugent score to correlate with the microbiota community composition, which was confirmed by 16S rDNA sequencing on a subset of samples. We then quantified the mobility of fluorescently-labeled HIV virions and nanoparticles to characterize the structural and adhesive barrier properties of CVM. Our analyses included women with Nugent scores categorized as intermediate (4-6) and BV (7-10), women that were either symptomatic or asymptomatic, and a small group of women before and after antibiotic treatment for symptomatic BV. Overall, we found that HIV virions had significantly increased mobility in CVM from women with BV compared to CVM from women with Lactobacillus crispatus-dominant microbiota, regardless of whether symptoms were present. We confirmed using nanoparticles and scanning electron microscopy that the impaired barrier function was due to reduced adhesive barrier properties without an obvious degradation of the physical CVM pore structure. We further confirmed a similar increase in HIV mobility in CVM from women with Lactobacillus iners-dominant microbiota, the species most associated with transitions to BV and that persists after antibiotic treatment for BV. Our findings advance the understanding of the protective role of mucus and highlight the interplay between vaginal microbiota and the innate barrier function mucus.https://doi.org/10.1371/journal.ppat.1008236 |
| spellingShingle | Thuy Hoang Emily Toler Kevin DeLong Nomfuneko A Mafunda Seth M Bloom Hannah C Zierden Thomas R Moench Jenell S Coleman Justin Hanes Douglas S Kwon Samuel K Lai Richard A Cone Laura M Ensign The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis. PLoS Pathogens |
| title | The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis. |
| title_full | The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis. |
| title_fullStr | The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis. |
| title_full_unstemmed | The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis. |
| title_short | The cervicovaginal mucus barrier to HIV-1 is diminished in bacterial vaginosis. |
| title_sort | cervicovaginal mucus barrier to hiv 1 is diminished in bacterial vaginosis |
| url | https://doi.org/10.1371/journal.ppat.1008236 |
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