The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves
Elastin is present in the extracellular matrix (ECM) of connective tissues, and its mechanical properties are well documented. In Marfan syndrome, however, the inability to properly code for the protein fibrillin-1 prematurely leads to the degradation and loss of elastin fiber integrity in the ECM....
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106221/?tool=EBI |
| _version_ | 1818009260735332352 |
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| author | Samuel D. Salinas Yasmeen M. Farra Keyvan Amini Khoiy James Houston Chung-Hao Lee Chiara Bellini Rouzbeh Amini |
| author_facet | Samuel D. Salinas Yasmeen M. Farra Keyvan Amini Khoiy James Houston Chung-Hao Lee Chiara Bellini Rouzbeh Amini |
| author_sort | Samuel D. Salinas |
| collection | DOAJ |
| description | Elastin is present in the extracellular matrix (ECM) of connective tissues, and its mechanical properties are well documented. In Marfan syndrome, however, the inability to properly code for the protein fibrillin-1 prematurely leads to the degradation and loss of elastin fiber integrity in the ECM. In this study, the role of elastin in the ECM of the anterior leaflet of the tricuspid valve was investigated by examining the biomechanical behavior of porcine leaflets before and after the application of the enzyme elastase. Five loading protocols were applied to the leaflet specimens in two groups (elastase-treated and control samples). The mechanical response following elastase application yielded a significantly stiffer material in both the radial and circumferential directions. At a physiological level of stress (85 kPa), the elastase group had an average strain of 26.21% and 6.32% in the radial and circumferential directions, respectively, at baseline prior to elastase application. Following elastase treatment, the average strain was 5.28% and 0.97% in the radial and circumferential directions, respectively. No statistically significant change was found in the control group following sham treatment with phosphate-buffered saline (PBS). Two-photon microscopy images confirmed that after the removal of elastin, the collagen fibers displayed a loss of undulation. With a significant reduction in radial compliance, the ability to withstand physiological loads may be compromised. As such, an extracellular matrix that is structurally deficient in elastin may hinder normal tricuspid valve function. |
| first_indexed | 2024-04-14T05:39:48Z |
| format | Article |
| id | doaj.art-ec5c79b761604440bacfbb4dfe65ca9d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T05:39:48Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ec5c79b761604440bacfbb4dfe65ca9d2022-12-22T02:09:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01175The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valvesSamuel D. SalinasYasmeen M. FarraKeyvan Amini KhoiyJames HoustonChung-Hao LeeChiara BelliniRouzbeh AminiElastin is present in the extracellular matrix (ECM) of connective tissues, and its mechanical properties are well documented. In Marfan syndrome, however, the inability to properly code for the protein fibrillin-1 prematurely leads to the degradation and loss of elastin fiber integrity in the ECM. In this study, the role of elastin in the ECM of the anterior leaflet of the tricuspid valve was investigated by examining the biomechanical behavior of porcine leaflets before and after the application of the enzyme elastase. Five loading protocols were applied to the leaflet specimens in two groups (elastase-treated and control samples). The mechanical response following elastase application yielded a significantly stiffer material in both the radial and circumferential directions. At a physiological level of stress (85 kPa), the elastase group had an average strain of 26.21% and 6.32% in the radial and circumferential directions, respectively, at baseline prior to elastase application. Following elastase treatment, the average strain was 5.28% and 0.97% in the radial and circumferential directions, respectively. No statistically significant change was found in the control group following sham treatment with phosphate-buffered saline (PBS). Two-photon microscopy images confirmed that after the removal of elastin, the collagen fibers displayed a loss of undulation. With a significant reduction in radial compliance, the ability to withstand physiological loads may be compromised. As such, an extracellular matrix that is structurally deficient in elastin may hinder normal tricuspid valve function.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106221/?tool=EBI |
| spellingShingle | Samuel D. Salinas Yasmeen M. Farra Keyvan Amini Khoiy James Houston Chung-Hao Lee Chiara Bellini Rouzbeh Amini The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves PLoS ONE |
| title | The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves |
| title_full | The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves |
| title_fullStr | The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves |
| title_full_unstemmed | The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves |
| title_short | The role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves |
| title_sort | role of elastin on the mechanical properties of the anterior leaflet in porcine tricuspid valves |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106221/?tool=EBI |
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