<i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy
<i>Nelumbo nucifera</i> Gaertn. (lotus) is an important medicinal plant, and many parts of the plant have been investigated for their therapeutic effects. However, the therapeutic effect of receptacles of lotuses on pathological cardiomyocyte hypertrophy has not been investigated yet. Th...
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/9/1647 |
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| author | Soyoung Cho Hyun Woo Cho Kyeong Wan Woo Jisu Jeong Juyeon Lim Sungha Park Miran Seo Soyeon Lim |
| author_facet | Soyoung Cho Hyun Woo Cho Kyeong Wan Woo Jisu Jeong Juyeon Lim Sungha Park Miran Seo Soyeon Lim |
| author_sort | Soyoung Cho |
| collection | DOAJ |
| description | <i>Nelumbo nucifera</i> Gaertn. (lotus) is an important medicinal plant, and many parts of the plant have been investigated for their therapeutic effects. However, the therapeutic effect of receptacles of lotuses on pathological cardiomyocyte hypertrophy has not been investigated yet. Therefore, the current study aimed to determine the protective effect of lotus against angiotensin II (Ang II)-induced cardiomyocyte hypertrophy in vitro. Ang II was used to induce hypertrophy of H9c2 cells. The lotus receptacle powder (MeOH extract of receptaculum Nelumbinis; MRN) used in the experiments was prepared by MeOH extraction and subsequent evaporation. To evaluate the effect of MRN on cardiomyocyte hypertrophy, cell size, protein synthesis, and hypertrophic marker expressions were examined. The antioxidant ability of MRN was determined by using CM-H<sub>2</sub>DCFDA, a general oxidative stress indicator. Ang II-induced cardiomyocyte hypertrophy was significantly attenuated by 5 µg/mL of MRN, as confirmed by the reductions in cell size, protein synthesis, and hypertrophic marker expression. MRN also attenuated Ang II-induced excessive intracellular reactive oxygen species (ROS) production through the suppression of protein kinase C (PKC), extracellular-signal-regulated kinase (ERK), and NF-κB activation and subsequent type I angiotensin receptor (AT1R), receptor for advanced glycation end products (RAGE), and NADPH oxidase (NOX) expression. MRN exerted a significant protective effect against Ang II-induced cardiomyocyte hypertrophy through suppression of PKC−ERK signaling, and this subsequently led to attenuation of intracellular ROS production. |
| first_indexed | 2024-12-23T19:39:19Z |
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| issn | 1420-3049 |
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| last_indexed | 2024-12-23T19:39:19Z |
| publishDate | 2019-04-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ec5ca8e786c949c9920b6d864790b5e22022-12-21T17:33:42ZengMDPI AGMolecules1420-30492019-04-01249164710.3390/molecules24091647molecules24091647<i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte HypertrophySoyoung Cho0Hyun Woo Cho1Kyeong Wan Woo2Jisu Jeong3Juyeon Lim4Sungha Park5Miran Seo6Soyeon Lim7Graduate Program in Science for Aging, Yonsei University, Seoul 120-752, KoreaKorean Medicinal Herbs Research Team, National Development Institute of Korean Medicine, 288 Udeuraendeu-gil, Jeollanam-do 59337, KoreaKorean Medicinal Herbs Research Team, National Development Institute of Korean Medicine, 288 Udeuraendeu-gil, Jeollanam-do 59337, KoreaGraduate Program in Science for Aging, Yonsei University, Seoul 120-752, KoreaGraduate Program in Science for Aging, Yonsei University, Seoul 120-752, KoreaCardiovascular Research Institute, Division of Cardiology, Yonsei University College of Medicine, Seoul 120-752, KoreaSeverance Integrative Research Institute for Cerebral & Cardiovascular Diseases, Yonsei University College of Medicine, Seoul 120-752, KoreaInstitute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung, Gangwon-do 25601, Korea<i>Nelumbo nucifera</i> Gaertn. (lotus) is an important medicinal plant, and many parts of the plant have been investigated for their therapeutic effects. However, the therapeutic effect of receptacles of lotuses on pathological cardiomyocyte hypertrophy has not been investigated yet. Therefore, the current study aimed to determine the protective effect of lotus against angiotensin II (Ang II)-induced cardiomyocyte hypertrophy in vitro. Ang II was used to induce hypertrophy of H9c2 cells. The lotus receptacle powder (MeOH extract of receptaculum Nelumbinis; MRN) used in the experiments was prepared by MeOH extraction and subsequent evaporation. To evaluate the effect of MRN on cardiomyocyte hypertrophy, cell size, protein synthesis, and hypertrophic marker expressions were examined. The antioxidant ability of MRN was determined by using CM-H<sub>2</sub>DCFDA, a general oxidative stress indicator. Ang II-induced cardiomyocyte hypertrophy was significantly attenuated by 5 µg/mL of MRN, as confirmed by the reductions in cell size, protein synthesis, and hypertrophic marker expression. MRN also attenuated Ang II-induced excessive intracellular reactive oxygen species (ROS) production through the suppression of protein kinase C (PKC), extracellular-signal-regulated kinase (ERK), and NF-κB activation and subsequent type I angiotensin receptor (AT1R), receptor for advanced glycation end products (RAGE), and NADPH oxidase (NOX) expression. MRN exerted a significant protective effect against Ang II-induced cardiomyocyte hypertrophy through suppression of PKC−ERK signaling, and this subsequently led to attenuation of intracellular ROS production.https://www.mdpi.com/1420-3049/24/9/1647<i>Nelumbo nucifera</i> receptaculumangiotensin IIcardiomyocyte hypertrophy |
| spellingShingle | Soyoung Cho Hyun Woo Cho Kyeong Wan Woo Jisu Jeong Juyeon Lim Sungha Park Miran Seo Soyeon Lim <i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy Molecules <i>Nelumbo nucifera</i> receptaculum angiotensin II cardiomyocyte hypertrophy |
| title | <i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy |
| title_full | <i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy |
| title_fullStr | <i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy |
| title_full_unstemmed | <i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy |
| title_short | <i>Nelumbo nucifera</i> Receptaculum Extract Suppresses Angiotensin II-Induced Cardiomyocyte Hypertrophy |
| title_sort | i nelumbo nucifera i receptaculum extract suppresses angiotensin ii induced cardiomyocyte hypertrophy |
| topic | <i>Nelumbo nucifera</i> receptaculum angiotensin II cardiomyocyte hypertrophy |
| url | https://www.mdpi.com/1420-3049/24/9/1647 |
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