Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activities
Biogenic Zinc oxide (ZnO) nanoparticles (NPs) were synthesized from Celosia argentea (C. argentea) plant extract. Structural analysis confirms the successful synthesis of biogenic zinc oxide NPs from C. argentea extract. The biogenic ZnO NPs have an average particle size of 21.55 ± 4.73 nm, a semisp...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1283898/full |
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| author | Rana Abdullah Alghamdi Rana Abdullah Alghamdi Maryam Hassan Al-Zahrani Lamaia R. Altarjami Waleed Al Abdulmonem Nadia Samir Alaa Said Ashjan A. Shami W. S. Mohamed W. S. Mohamed Mohammed Ezzeldien Mohammed Ezzeldien |
| author_facet | Rana Abdullah Alghamdi Rana Abdullah Alghamdi Maryam Hassan Al-Zahrani Lamaia R. Altarjami Waleed Al Abdulmonem Nadia Samir Alaa Said Ashjan A. Shami W. S. Mohamed W. S. Mohamed Mohammed Ezzeldien Mohammed Ezzeldien |
| author_sort | Rana Abdullah Alghamdi |
| collection | DOAJ |
| description | Biogenic Zinc oxide (ZnO) nanoparticles (NPs) were synthesized from Celosia argentea (C. argentea) plant extract. Structural analysis confirms the successful synthesis of biogenic zinc oxide NPs from C. argentea extract. The biogenic ZnO NPs have an average particle size of 21.55 ± 4.73 nm, a semispherical shape, and a specific surface area of about 50 m2/g. The biogenic ZnO NPs have a powerful radical scavenging activity (Ic50 = 91.24 mg/ml) comparable to ascorbic acid (ASC) as a standard (Ic50 = 14.37 mg/ml). The antibacterial efficacy was tested against gram-positive and gram-negative bacteria using an agar disc diffusion method. Gram-positive strains with biogenic ZnO NPs have a greater bactericidal impact than gram-negative strains in a concentration-dependent manner. Anticancer activity against Liver hepatocellular cells (HepG2) and Human umbilical vein endothelial cells (HUVEC) was evaluated using a [3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide] (MTT) assay. The results reflect the concentration-dependent cytotoxic effect of biogenic ZnO NPs against HepG2 cells even at low concentrations (Ic50 = 49.45 μg/ml) compared with doxorubicin (Ic50 = 14.67 μg/ml) and C. argentea extract (Ic50 = 112.24 μg/ml). The cell cycle and gene expression were analyzed to determine the potential anticancer mechanism. The flow cytometric analysis of the cell cycle revealed that biogenic ZnO NPs induce oxidative stress that activates the apoptotic genes NF-κB, CY-C, and P53, leading to cell death. The Celosia argentea improved the antioxidant, antibacterial, and anticancer activities of ZnO NPs without altering their structural properties. The effect of green synthesis on the bioactivity of biogenic ZnO NPs in vivo is recommended for future work. |
| first_indexed | 2024-03-08T23:06:59Z |
| format | Article |
| id | doaj.art-ec5e26e788ba4f2b8acff97755fd07e2 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T23:06:59Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ec5e26e788ba4f2b8acff97755fd07e22023-12-15T11:06:33ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-12-011110.3389/fbioe.2023.12838981283898Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activitiesRana Abdullah Alghamdi0Rana Abdullah Alghamdi1Maryam Hassan Al-Zahrani2Lamaia R. Altarjami3Waleed Al Abdulmonem4Nadia Samir5Alaa Said6Ashjan A. Shami7W. S. Mohamed8W. S. Mohamed9Mohammed Ezzeldien10Mohammed Ezzeldien11Department of Chemistry, College of Sciences and Arts, King Abdulaziz University, Rabigh, Saudi ArabiaRegenerative Medicine Unit, King Fahd Medical Research Centre, King Abdulaziz University, Jeddah, Saudi ArabiaBiochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Chemistry, College of Sciences and Arts, King Abdulaziz University, Rabigh, Saudi ArabiaDepartment of Pathology, College of Medicine, Qassim University, Buraidah, Saudi ArabiaZoology Department, Faculty of Science, South Valley University, Qena, EgyptElectronic and Nano Devises Lab, Faculty of Science, South Valley University, Qena, EgyptDepartment of clinical laboratory sciences, College of applied medical sciences, Taif University, Taif, Saudi ArabiaPhysics Department, College of Science, Jouf University, Al-Jouf, Sakaka, Saudi ArabiaPhysics Department, Faculty of Science, Sohag University, Sohag, EgyptPhysics Department, College of Science, Jouf University, Al-Jouf, Sakaka, Saudi Arabia0Metallurgy and Material Science Tests Lab, Physics Department, Faculty of Science, South Valley University, Qena, EgyptBiogenic Zinc oxide (ZnO) nanoparticles (NPs) were synthesized from Celosia argentea (C. argentea) plant extract. Structural analysis confirms the successful synthesis of biogenic zinc oxide NPs from C. argentea extract. The biogenic ZnO NPs have an average particle size of 21.55 ± 4.73 nm, a semispherical shape, and a specific surface area of about 50 m2/g. The biogenic ZnO NPs have a powerful radical scavenging activity (Ic50 = 91.24 mg/ml) comparable to ascorbic acid (ASC) as a standard (Ic50 = 14.37 mg/ml). The antibacterial efficacy was tested against gram-positive and gram-negative bacteria using an agar disc diffusion method. Gram-positive strains with biogenic ZnO NPs have a greater bactericidal impact than gram-negative strains in a concentration-dependent manner. Anticancer activity against Liver hepatocellular cells (HepG2) and Human umbilical vein endothelial cells (HUVEC) was evaluated using a [3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide] (MTT) assay. The results reflect the concentration-dependent cytotoxic effect of biogenic ZnO NPs against HepG2 cells even at low concentrations (Ic50 = 49.45 μg/ml) compared with doxorubicin (Ic50 = 14.67 μg/ml) and C. argentea extract (Ic50 = 112.24 μg/ml). The cell cycle and gene expression were analyzed to determine the potential anticancer mechanism. The flow cytometric analysis of the cell cycle revealed that biogenic ZnO NPs induce oxidative stress that activates the apoptotic genes NF-κB, CY-C, and P53, leading to cell death. The Celosia argentea improved the antioxidant, antibacterial, and anticancer activities of ZnO NPs without altering their structural properties. The effect of green synthesis on the bioactivity of biogenic ZnO NPs in vivo is recommended for future work.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1283898/fullCelosia argenteaZnO NPsantioxidantantibacterialanticancer |
| spellingShingle | Rana Abdullah Alghamdi Rana Abdullah Alghamdi Maryam Hassan Al-Zahrani Lamaia R. Altarjami Waleed Al Abdulmonem Nadia Samir Alaa Said Ashjan A. Shami W. S. Mohamed W. S. Mohamed Mohammed Ezzeldien Mohammed Ezzeldien Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activities Frontiers in Bioengineering and Biotechnology Celosia argentea ZnO NPs antioxidant antibacterial anticancer |
| title | Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activities |
| title_full | Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activities |
| title_fullStr | Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activities |
| title_full_unstemmed | Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activities |
| title_short | Biogenic Zinc oxide nanoparticles from Celosia argentea: toward improved antioxidant, antibacterial, and anticancer activities |
| title_sort | biogenic zinc oxide nanoparticles from celosia argentea toward improved antioxidant antibacterial and anticancer activities |
| topic | Celosia argentea ZnO NPs antioxidant antibacterial anticancer |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1283898/full |
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