A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in rats
Background: Abhrak bhasma (AB) is widely used in the treatment of diabetes mellitus (DM); however, no scientific study is reported till date on its efficacy and safety to prove it's pharmacological effects. Streptozotocin (STZ) is a gulcosamine–nitrosourea complex produced by Streptomyces achro...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Journal of Ayurveda and Integrative Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0975947621000486 |
| _version_ | 1819090884466049024 |
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| author | Harish Gopinath Murugesh Shivashankar |
| author_facet | Harish Gopinath Murugesh Shivashankar |
| author_sort | Harish Gopinath |
| collection | DOAJ |
| description | Background: Abhrak bhasma (AB) is widely used in the treatment of diabetes mellitus (DM); however, no scientific study is reported till date on its efficacy and safety to prove it's pharmacological effects. Streptozotocin (STZ) is a gulcosamine–nitrosourea complex produced by Streptomyces achromogenes, which particularly induces DNA strand breakage in pancreatic β-cells causing DM. The damage caused by STZ to pancreatic β-cells is coupled with insulin release in the initial stage, subsequently leading to hyperglycemia owing to insulin deficiency. Objective(s): In the present study, the safety and effectiveness of AB was evaluated by in vivo acute and sub-acute toxicity study and STZ-induced hyperglycemic activity in rats. Materials and methods: Acute oral toxicity assessed as per OECD 425 guidelines by employing up-down procedure (UDP), sub-acute toxicity as per OECD-407 guidelines and anti-hyperglycemic activity using STZ method. The anti-hyperglycemic potential of AB in rats (40, 80, and 160 mg/kg body weight (b.w.)) was evaluated by determining the body weight, blood glucose, organ weight, lipid profile, and histo-morphological and histo-pathological investigations. Results: The highest tolerated dose of AB was 2000 mg/kg b.w., and sub-acute toxicity of different AB doses showed no significant variation, when compared with the control. Interestingly, a noteworthy reduction in blood glucose, total cholesterol, and triglycerides levels were observed in AB-treated diabetic rats, along with a considerable increase in body weight, when compared with those noted in the disease control and normal control. Conclusion: Effectiveness of AB as a potential safe and potent candidate for the treatment of diabetes was revealed as compared to the standard drug Metformin by STZ-induced method. |
| first_indexed | 2024-12-21T22:30:55Z |
| format | Article |
| id | doaj.art-ec5e8d23c1404e57a843bdbf0fd878fe |
| institution | Directory Open Access Journal |
| issn | 0975-9476 |
| language | English |
| last_indexed | 2024-12-21T22:30:55Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ayurveda and Integrative Medicine |
| spelling | doaj.art-ec5e8d23c1404e57a843bdbf0fd878fe2022-12-21T18:48:05ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762021-07-01123443451A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in ratsHarish Gopinath0Murugesh Shivashankar1Department of Chemistry, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, IndiaCorresponding author.; Department of Chemistry, Vellore Institute of Technology, Vellore 632014, Tamil Nadu, IndiaBackground: Abhrak bhasma (AB) is widely used in the treatment of diabetes mellitus (DM); however, no scientific study is reported till date on its efficacy and safety to prove it's pharmacological effects. Streptozotocin (STZ) is a gulcosamine–nitrosourea complex produced by Streptomyces achromogenes, which particularly induces DNA strand breakage in pancreatic β-cells causing DM. The damage caused by STZ to pancreatic β-cells is coupled with insulin release in the initial stage, subsequently leading to hyperglycemia owing to insulin deficiency. Objective(s): In the present study, the safety and effectiveness of AB was evaluated by in vivo acute and sub-acute toxicity study and STZ-induced hyperglycemic activity in rats. Materials and methods: Acute oral toxicity assessed as per OECD 425 guidelines by employing up-down procedure (UDP), sub-acute toxicity as per OECD-407 guidelines and anti-hyperglycemic activity using STZ method. The anti-hyperglycemic potential of AB in rats (40, 80, and 160 mg/kg body weight (b.w.)) was evaluated by determining the body weight, blood glucose, organ weight, lipid profile, and histo-morphological and histo-pathological investigations. Results: The highest tolerated dose of AB was 2000 mg/kg b.w., and sub-acute toxicity of different AB doses showed no significant variation, when compared with the control. Interestingly, a noteworthy reduction in blood glucose, total cholesterol, and triglycerides levels were observed in AB-treated diabetic rats, along with a considerable increase in body weight, when compared with those noted in the disease control and normal control. Conclusion: Effectiveness of AB as a potential safe and potent candidate for the treatment of diabetes was revealed as compared to the standard drug Metformin by STZ-induced method.http://www.sciencedirect.com/science/article/pii/S0975947621000486Abhrak bhasmaAnti-diabeticSTZ inducedAcute toxicitySub-acute toxicity |
| spellingShingle | Harish Gopinath Murugesh Shivashankar A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in rats Journal of Ayurveda and Integrative Medicine Abhrak bhasma Anti-diabetic STZ induced Acute toxicity Sub-acute toxicity |
| title | A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in rats |
| title_full | A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in rats |
| title_fullStr | A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in rats |
| title_full_unstemmed | A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in rats |
| title_short | A study on toxicity and anti-hyperglycemic effects of Abhrak Bhasma in rats |
| title_sort | study on toxicity and anti hyperglycemic effects of abhrak bhasma in rats |
| topic | Abhrak bhasma Anti-diabetic STZ induced Acute toxicity Sub-acute toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0975947621000486 |
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