Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and Lipopolysaccharide
Gender differences in the hemocyte immune response of Hong Kong oyster <i>Crassostrea hongkongensis</i> to <i>Vibrio harveyi</i> and lipopolysaccharide (LPS) infection exist. To determine if a gender difference also exists, we use a <sup>1</sup>H NMR-based metabol...
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MDPI AG
2022-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/6/1178 |
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| author | Lijuan Ma Jie Lu Tuo Yao Lingtong Ye Jiangyong Wang |
| author_facet | Lijuan Ma Jie Lu Tuo Yao Lingtong Ye Jiangyong Wang |
| author_sort | Lijuan Ma |
| collection | DOAJ |
| description | Gender differences in the hemocyte immune response of Hong Kong oyster <i>Crassostrea hongkongensis</i> to <i>Vibrio harveyi</i> and lipopolysaccharide (LPS) infection exist. To determine if a gender difference also exists, we use a <sup>1</sup>H NMR-based metabolomics method to investigate responses in <i>C. hongkongensis</i> hepatopancreas tissues to <i>V. harveyi</i> and LPS infection. Both infections induced pronounced gender- and immune-specific metabolic responses in hepatopancreas tissues. Responses are mainly presented in changes in substances involved in energy metabolism (decreased glucose, ATP, and AMP in males and increased ATP and AMP in LPS-infected females), oxidative stress (decreased glutathione in males and decreased tryptophan and phenylalanine and increased choline and proline in LPS-infected females), tricarboxylic acid (TCA) cycle (decreased α-ketoglutarate acid and increased fumarate in LPS-infected males, and decreased fumarate in LPS-infected females), and osmotic regulation (decreased trigonelline and increased taurine in <i>V. harveyi</i>-infected males and decreased betaine in <i>V. harveyi</i>-infected females). Results suggest that post-spawning-phase male oysters have a more significant energy metabolic response and greater ability to cope with oxidative stress than female oysters. We propose that the impact of oyster gender should be taken into consideration in the aftermath of oyster farming or oyster disease in natural seas. |
| first_indexed | 2024-03-10T00:33:56Z |
| format | Article |
| id | doaj.art-ec5ea8380c684bb485b1bd1efac2a347 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T00:33:56Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-ec5ea8380c684bb485b1bd1efac2a3472023-11-23T15:20:58ZengMDPI AGAntioxidants2076-39212022-06-01116117810.3390/antiox11061178Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and LipopolysaccharideLijuan Ma0Jie Lu1Tuo Yao2Lingtong Ye3Jiangyong Wang4Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaSchool of Life Science, Huizhou University, Huizhou 516007, ChinaGender differences in the hemocyte immune response of Hong Kong oyster <i>Crassostrea hongkongensis</i> to <i>Vibrio harveyi</i> and lipopolysaccharide (LPS) infection exist. To determine if a gender difference also exists, we use a <sup>1</sup>H NMR-based metabolomics method to investigate responses in <i>C. hongkongensis</i> hepatopancreas tissues to <i>V. harveyi</i> and LPS infection. Both infections induced pronounced gender- and immune-specific metabolic responses in hepatopancreas tissues. Responses are mainly presented in changes in substances involved in energy metabolism (decreased glucose, ATP, and AMP in males and increased ATP and AMP in LPS-infected females), oxidative stress (decreased glutathione in males and decreased tryptophan and phenylalanine and increased choline and proline in LPS-infected females), tricarboxylic acid (TCA) cycle (decreased α-ketoglutarate acid and increased fumarate in LPS-infected males, and decreased fumarate in LPS-infected females), and osmotic regulation (decreased trigonelline and increased taurine in <i>V. harveyi</i>-infected males and decreased betaine in <i>V. harveyi</i>-infected females). Results suggest that post-spawning-phase male oysters have a more significant energy metabolic response and greater ability to cope with oxidative stress than female oysters. We propose that the impact of oyster gender should be taken into consideration in the aftermath of oyster farming or oyster disease in natural seas.https://www.mdpi.com/2076-3921/11/6/1178<i>Crassostrea hongkongensis</i>metabolomicshepatopancreasgender-based difference |
| spellingShingle | Lijuan Ma Jie Lu Tuo Yao Lingtong Ye Jiangyong Wang Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and Lipopolysaccharide Antioxidants <i>Crassostrea hongkongensis</i> metabolomics hepatopancreas gender-based difference |
| title | Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and Lipopolysaccharide |
| title_full | Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and Lipopolysaccharide |
| title_fullStr | Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and Lipopolysaccharide |
| title_full_unstemmed | Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and Lipopolysaccharide |
| title_short | Gender-Specific Metabolic Responses of <i>Crassostrea hongkongensis</i> to Infection with <i>Vibrio harveyi</i> and Lipopolysaccharide |
| title_sort | gender specific metabolic responses of i crassostrea hongkongensis i to infection with i vibrio harveyi i and lipopolysaccharide |
| topic | <i>Crassostrea hongkongensis</i> metabolomics hepatopancreas gender-based difference |
| url | https://www.mdpi.com/2076-3921/11/6/1178 |
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