| Summary: | <p>Abstract</p> <p>Background</p> <p>The definitive indicator of Alzheimer’s disease (AD) pathology is the profuse accumulation of amyloid-ß (Aß) within the brain. Various <it>in vitro</it> and cell-based models have been proposed for high throughput drug screening for potential therapeutic benefit in diseases of protein misfolding. <it>Caenorhabditis elegans</it> offers a convenient <it>in vivo</it> system for examination of Aß accumulation and toxicity in a complex multicellular organism. Ease of culturing and a short life cycle make this animal model well suited to rapid screening of candidate compounds.</p> <p>Results</p> <p>We have generated a new transgenic strain of <it>C. elegans</it> that expresses full length Aß<sub>1-42</sub>. This strain differs from existing Aß models that predominantly express amino-truncated Aß<sub>3-42</sub>. The Aß<sub>1-42</sub> is expressed in body wall muscle cells, where it oligomerizes, aggregates and results in severe, and fully penetrant, age progressive-paralysis. The <it>in vivo</it> accumulation of Aß<sub>1-42</sub> also stains positive for amyloid dyes, consistent with <it>in vivo</it> fibril formation. The utility of this model for identification of potential protective compounds was examined using the investigational Alzheimer’s therapeutic PBT2, shown to be neuroprotective in mouse models of AD and significantly improve cognition in AD patients. We observed that treatment with PBT2 provided rapid and significant protection against the Aß-induced toxicity in <it>C. elegans</it>.</p> <p>Conclusion</p> <p>This <it>C. elegans</it> model of full length Aß<sub>1-42</sub> expression can now be adopted for use in screens to rapidly identify and assist in development of potential therapeutics and to study underlying toxic mechanism(s) of Aß.</p>
|
|---|