Effect of RNA interference on WD101 gene of Schistosoma japonicum
Objective: To study the effect of RNA interference (RNAi) on WD101 gene and its effect on the expression of WD101 mRNA and protein in Schistosoma japonicum. Methods: Double-stranded RNA (dsRNA) WD101 gene and control gene (lacZ) were generated by in vitro transcription and transfected into mechanica...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
| Subjects: | |
| Online Access: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=136;epage=140;aulast=Zhang |
| _version_ | 1828471637164425216 |
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| author | Peng Zhang Gang Feng Wei-Na Zhang Ying-Ying Zhang Chun-Sheng Liu |
| author_facet | Peng Zhang Gang Feng Wei-Na Zhang Ying-Ying Zhang Chun-Sheng Liu |
| author_sort | Peng Zhang |
| collection | DOAJ |
| description | Objective: To study the effect of RNA interference (RNAi) on WD101 gene and its effect on the expression of WD101 mRNA and protein in Schistosoma japonicum. Methods: Double-stranded RNA (dsRNA) WD101 gene and control gene (lacZ) were generated by in vitro transcription and transfected into mechanically transformed schistosomula. The total RNA and protein were isolated simultaneously using TRIzol reagent. The expression levels of mRNA and the protein were determined by quantitative real-time PCR (qPCR) and Western blotting, respectively. After injected dsRNA-electroporated schistosomula into BALB/c mouse six weeks, the male and female reproductive organs were observed and measured under the confocal laser scanning microscope. Results: After 1, 3 and 5 d of RNAi, WD101 mRNA level was decreased by 15%, 39%, and 58% in experiment group compared to that in control group; meanwhile, WD101 protein level was decreased by 11%, 28%, and 43% in experiment group compared to that in control group. There were significantly more sperms in testicular lobes in experiment group than that in control group, while there were no significant differences in terms of ovary and vitelline glands between two groups. Conclusions: The dsWD101-RNAi can effectively induce suppression of WD101 gene expression at both mRNA and protein levels. WD101 gene might be a reproduction-related gene in Schistosoma japonicum. |
| first_indexed | 2024-12-11T05:14:40Z |
| format | Article |
| id | doaj.art-ec641deb435b41589b798eb327f39377 |
| institution | Directory Open Access Journal |
| issn | 2352-4146 |
| language | English |
| last_indexed | 2024-12-11T05:14:40Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj.art-ec641deb435b41589b798eb327f393772022-12-22T01:19:50ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462018-01-0111213614010.4103/1995-7645.225021Effect of RNA interference on WD101 gene of Schistosoma japonicumPeng ZhangGang FengWei-Na ZhangYing-Ying ZhangChun-Sheng LiuObjective: To study the effect of RNA interference (RNAi) on WD101 gene and its effect on the expression of WD101 mRNA and protein in Schistosoma japonicum. Methods: Double-stranded RNA (dsRNA) WD101 gene and control gene (lacZ) were generated by in vitro transcription and transfected into mechanically transformed schistosomula. The total RNA and protein were isolated simultaneously using TRIzol reagent. The expression levels of mRNA and the protein were determined by quantitative real-time PCR (qPCR) and Western blotting, respectively. After injected dsRNA-electroporated schistosomula into BALB/c mouse six weeks, the male and female reproductive organs were observed and measured under the confocal laser scanning microscope. Results: After 1, 3 and 5 d of RNAi, WD101 mRNA level was decreased by 15%, 39%, and 58% in experiment group compared to that in control group; meanwhile, WD101 protein level was decreased by 11%, 28%, and 43% in experiment group compared to that in control group. There were significantly more sperms in testicular lobes in experiment group than that in control group, while there were no significant differences in terms of ovary and vitelline glands between two groups. Conclusions: The dsWD101-RNAi can effectively induce suppression of WD101 gene expression at both mRNA and protein levels. WD101 gene might be a reproduction-related gene in Schistosoma japonicum.http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=136;epage=140;aulast=Zhangschistosoma japonicumrna interferencewd101 |
| spellingShingle | Peng Zhang Gang Feng Wei-Na Zhang Ying-Ying Zhang Chun-Sheng Liu Effect of RNA interference on WD101 gene of Schistosoma japonicum Asian Pacific Journal of Tropical Medicine schistosoma japonicum rna interference wd101 |
| title | Effect of RNA interference on WD101 gene of Schistosoma japonicum |
| title_full | Effect of RNA interference on WD101 gene of Schistosoma japonicum |
| title_fullStr | Effect of RNA interference on WD101 gene of Schistosoma japonicum |
| title_full_unstemmed | Effect of RNA interference on WD101 gene of Schistosoma japonicum |
| title_short | Effect of RNA interference on WD101 gene of Schistosoma japonicum |
| title_sort | effect of rna interference on wd101 gene of schistosoma japonicum |
| topic | schistosoma japonicum rna interference wd101 |
| url | http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=2;spage=136;epage=140;aulast=Zhang |
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