Separation of propranolol enantiomers using chiral HPLC
Enantiomers of pharmaceuticals may exhibit different effects because the organism serves as a chiral selector. Enantiomers can now be identified and separated using analytical techniques such as gas chromatography and high-performance liquid chromatography. The nonselective b-adrenergic blocker prop...
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| Format: | Article |
| Language: | English |
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University of Kragujevac, Faculty of Science
2023-01-01
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| Series: | Kragujevac Journal of Science |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2023/1450-96362345041T.pdf |
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| author | Turković Nemanja M. Savić Jasna Ivković Branka Ivković Aleksandar |
| author_facet | Turković Nemanja M. Savić Jasna Ivković Branka Ivković Aleksandar |
| author_sort | Turković Nemanja M. |
| collection | DOAJ |
| description | Enantiomers of pharmaceuticals may exhibit different effects because the organism serves as a chiral selector. Enantiomers can now be identified and separated using analytical techniques such as gas chromatography and high-performance liquid chromatography. The nonselective b-adrenergic blocker propranolol is used as a racemate in therapy, but only the S(-) isomer has been shown to be active. The aim of this study was to determine the mobile phase composition that would achieve optimal retention and satisfactory resolution between enantiomers and to confirm the order of elution of the enantiomers. The enantioseparation was tested in the n-heptane/ethanol/ammonia, n-heptane/ethanol/diethylamine, n-heptane/isopropanol/ ammonia and n-heptane/isopropanol/diethylamine systems. Optimal separation of enantiomers (t1=4,708; t2=5.264; Rs=1.75) was achieved with the mobile phase n-heptane/ethanol/diethylamine 80/20/0.1. The elution order was based on the retention time of the pure S(-)isomer. It was confirmed that the R(+) isomer shows higher retention. |
| first_indexed | 2024-03-12T14:59:57Z |
| format | Article |
| id | doaj.art-ec6580f21fc64a6883ee12dfd5b901b8 |
| institution | Directory Open Access Journal |
| issn | 1450-9636 2466-5509 |
| language | English |
| last_indexed | 2024-03-12T14:59:57Z |
| publishDate | 2023-01-01 |
| publisher | University of Kragujevac, Faculty of Science |
| record_format | Article |
| series | Kragujevac Journal of Science |
| spelling | doaj.art-ec6580f21fc64a6883ee12dfd5b901b82023-08-14T09:28:40ZengUniversity of Kragujevac, Faculty of ScienceKragujevac Journal of Science1450-96362466-55092023-01-0120234541491450-96362345041TSeparation of propranolol enantiomers using chiral HPLCTurković Nemanja M.0Savić Jasna1Ivković Branka2https://orcid.org/0000-0001-7350-3483Ivković Aleksandar3Institute for Medicines and Medical Devices of Montenegro (CInMED), Podgorica, MontenegroSchool of Pharmacy and Physiotherapy, Belgrade, SerbiaUniversity of Belgrade, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Belgrade, SerbiaAcademy of Technical Vocational Studies Belgrade, Department of Applied Engineering Sciences Požarevac, Požarevac, SerbiaEnantiomers of pharmaceuticals may exhibit different effects because the organism serves as a chiral selector. Enantiomers can now be identified and separated using analytical techniques such as gas chromatography and high-performance liquid chromatography. The nonselective b-adrenergic blocker propranolol is used as a racemate in therapy, but only the S(-) isomer has been shown to be active. The aim of this study was to determine the mobile phase composition that would achieve optimal retention and satisfactory resolution between enantiomers and to confirm the order of elution of the enantiomers. The enantioseparation was tested in the n-heptane/ethanol/ammonia, n-heptane/ethanol/diethylamine, n-heptane/isopropanol/ ammonia and n-heptane/isopropanol/diethylamine systems. Optimal separation of enantiomers (t1=4,708; t2=5.264; Rs=1.75) was achieved with the mobile phase n-heptane/ethanol/diethylamine 80/20/0.1. The elution order was based on the retention time of the pure S(-)isomer. It was confirmed that the R(+) isomer shows higher retention.https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2023/1450-96362345041T.pdfpropranololenantioseparationchiral stationary phaseshplc |
| spellingShingle | Turković Nemanja M. Savić Jasna Ivković Branka Ivković Aleksandar Separation of propranolol enantiomers using chiral HPLC Kragujevac Journal of Science propranolol enantioseparation chiral stationary phases hplc |
| title | Separation of propranolol enantiomers using chiral HPLC |
| title_full | Separation of propranolol enantiomers using chiral HPLC |
| title_fullStr | Separation of propranolol enantiomers using chiral HPLC |
| title_full_unstemmed | Separation of propranolol enantiomers using chiral HPLC |
| title_short | Separation of propranolol enantiomers using chiral HPLC |
| title_sort | separation of propranolol enantiomers using chiral hplc |
| topic | propranolol enantioseparation chiral stationary phases hplc |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2023/1450-96362345041T.pdf |
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