Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteria
<p>Abstract</p> <p>Background</p> <p>The six organic solvent extracts of <it>Artemisia nilagirica </it>were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains.</p> <...
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| Format: | Article |
| Language: | English |
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BMC
2010-01-01
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| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/10/6 |
| _version_ | 1818529138071306240 |
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| author | Hopper Waheeta Ahameethunisa Abdul R |
| author_facet | Hopper Waheeta Ahameethunisa Abdul R |
| author_sort | Hopper Waheeta |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The six organic solvent extracts of <it>Artemisia nilagirica </it>were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains.</p> <p>Methods</p> <p>The agar disk diffusion method was used to study the antibacterial activity of <it>A. nilagirica </it>extracts against 15 bacterial strains. The Minimum Inhibitory Concentration (MIC) of the plant extracts were tested using two fold agar dilution method at concentrations ranging from 32 to 512 μg/ml. The phytochemical screening of extracts was carried out for major phytochemical derivatives in <it>A. nilagirica</it>.</p> <p>Results</p> <p>All the extracts showed inhibitory activity for gram-positive and gram-negative bacteria except for <it>Klebsiella pneumoniae, Enterococcus faecalis </it>and <it>Staphylococcus aureus</it>. The hexane extract was found to be effective against all phytopathogens with low MIC of 32 μg/ml and the methanol extract exhibited a higher inhibition activity against <it>Escherichia coli, Yersinia enterocolitica, Salmonella typhi</it>, <it>Enterobacter aerogenes</it>, <it>Proteus vulgaris</it>, <it>Pseudomonas aeruginosa </it>(32 μg/ml), <it>Bacillus subtilis </it>(64 μg/ml) and <it>Shigella flaxneri </it>(128 μg/ml). The phytochemical screening of extracts answered for the major derivative of alkaloids, amino acids, flavonoids, phenol, quinines, tannins and terpenoids.</p> <p>Conclusion</p> <p>All the extracts showed antibacterial activity against the tested strains. Of all, methanol and hexane extracts showed high inhibition against clinical and phytopathogens, respectively. The results also indicate the presence of major phytochemical derivatives in the <it>A. nilagirica </it>extracts. Hence, the isolation and purification of therapeutic potential compounds from <it>A. nilagirica </it>could be used as an effective source against bacterial diseases in human and plants.</p> |
| first_indexed | 2024-12-11T11:59:02Z |
| format | Article |
| id | doaj.art-ec66112c7a714643b04c6dbf5dd513f3 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-11T11:59:02Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-ec66112c7a714643b04c6dbf5dd513f32022-12-22T01:08:08ZengBMCBMC Complementary and Alternative Medicine1472-68822010-01-01101610.1186/1472-6882-10-6Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteriaHopper WaheetaAhameethunisa Abdul R<p>Abstract</p> <p>Background</p> <p>The six organic solvent extracts of <it>Artemisia nilagirica </it>were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains.</p> <p>Methods</p> <p>The agar disk diffusion method was used to study the antibacterial activity of <it>A. nilagirica </it>extracts against 15 bacterial strains. The Minimum Inhibitory Concentration (MIC) of the plant extracts were tested using two fold agar dilution method at concentrations ranging from 32 to 512 μg/ml. The phytochemical screening of extracts was carried out for major phytochemical derivatives in <it>A. nilagirica</it>.</p> <p>Results</p> <p>All the extracts showed inhibitory activity for gram-positive and gram-negative bacteria except for <it>Klebsiella pneumoniae, Enterococcus faecalis </it>and <it>Staphylococcus aureus</it>. The hexane extract was found to be effective against all phytopathogens with low MIC of 32 μg/ml and the methanol extract exhibited a higher inhibition activity against <it>Escherichia coli, Yersinia enterocolitica, Salmonella typhi</it>, <it>Enterobacter aerogenes</it>, <it>Proteus vulgaris</it>, <it>Pseudomonas aeruginosa </it>(32 μg/ml), <it>Bacillus subtilis </it>(64 μg/ml) and <it>Shigella flaxneri </it>(128 μg/ml). The phytochemical screening of extracts answered for the major derivative of alkaloids, amino acids, flavonoids, phenol, quinines, tannins and terpenoids.</p> <p>Conclusion</p> <p>All the extracts showed antibacterial activity against the tested strains. Of all, methanol and hexane extracts showed high inhibition against clinical and phytopathogens, respectively. The results also indicate the presence of major phytochemical derivatives in the <it>A. nilagirica </it>extracts. Hence, the isolation and purification of therapeutic potential compounds from <it>A. nilagirica </it>could be used as an effective source against bacterial diseases in human and plants.</p>http://www.biomedcentral.com/1472-6882/10/6 |
| spellingShingle | Hopper Waheeta Ahameethunisa Abdul R Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteria BMC Complementary and Alternative Medicine |
| title | Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteria |
| title_full | Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteria |
| title_fullStr | Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteria |
| title_full_unstemmed | Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteria |
| title_short | Antibacterial activity of <it>Artemisia nilagirica </it>leaf extracts against clinical and phytopathogenic bacteria |
| title_sort | antibacterial activity of it artemisia nilagirica it leaf extracts against clinical and phytopathogenic bacteria |
| url | http://www.biomedcentral.com/1472-6882/10/6 |
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