Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells
Host released alarmins and antimicrobial peptides (AMPs) are highly effective as antifungal agents and inducers. Whilst some are expressed constitutively at mucosal tissues, the primary site of many infections, others are elicited in response to pathogens. In the context of <i>Candida albicans...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-03-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/3/699 |
| _version_ | 1827834287286648832 |
|---|---|
| author | Jemima Ho Don N. Wickramasinghe Spyridoula-Angeliki Nikou Bernhard Hube Jonathan P. Richardson Julian R. Naglik |
| author_facet | Jemima Ho Don N. Wickramasinghe Spyridoula-Angeliki Nikou Bernhard Hube Jonathan P. Richardson Julian R. Naglik |
| author_sort | Jemima Ho |
| collection | DOAJ |
| description | Host released alarmins and antimicrobial peptides (AMPs) are highly effective as antifungal agents and inducers. Whilst some are expressed constitutively at mucosal tissues, the primary site of many infections, others are elicited in response to pathogens. In the context of <i>Candida albicans</i>, the fungal factors inducing the release of these innate immune molecules are poorly defined. Herein, we identify candidalysin as a potent trigger of several key alarmins and AMPs known to possess potent anti-<i>Candida</i> functions. We also find extracellular ATP to be an important activator of candidalysin-induced epithelial signalling responses, namely epidermal growth factor receptor (EGFR) and MAPK signalling, which mediate downstream innate immunity during oral epithelial infection. The data provide novel mechanistic insight into the induction of multiple key alarmins and AMPs, important for antifungal defences against <i>C. albicans</i>. |
| first_indexed | 2024-03-12T05:48:04Z |
| format | Article |
| id | doaj.art-ec668f7636e94b7ca8c888cc55f436a6 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T05:48:04Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-ec668f7636e94b7ca8c888cc55f436a62023-09-03T05:27:59ZengMDPI AGCells2073-44092020-03-019369910.3390/cells9030699cells9030699Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial CellsJemima Ho0Don N. Wickramasinghe1Spyridoula-Angeliki Nikou2Bernhard Hube3Jonathan P. Richardson4Julian R. Naglik5Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 1UL, UKCentre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 1UL, UKCentre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 1UL, UKDepartment of Microbial Pathogenicity Mechanisms, Leibniz Institute for Natural Product Research and Infection Biology–Hans Knöll Institute (HKI), 07745 Jena, GermanyCentre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 1UL, UKCentre for Host-Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 1UL, UKHost released alarmins and antimicrobial peptides (AMPs) are highly effective as antifungal agents and inducers. Whilst some are expressed constitutively at mucosal tissues, the primary site of many infections, others are elicited in response to pathogens. In the context of <i>Candida albicans</i>, the fungal factors inducing the release of these innate immune molecules are poorly defined. Herein, we identify candidalysin as a potent trigger of several key alarmins and AMPs known to possess potent anti-<i>Candida</i> functions. We also find extracellular ATP to be an important activator of candidalysin-induced epithelial signalling responses, namely epidermal growth factor receptor (EGFR) and MAPK signalling, which mediate downstream innate immunity during oral epithelial infection. The data provide novel mechanistic insight into the induction of multiple key alarmins and AMPs, important for antifungal defences against <i>C. albicans</i>.https://www.mdpi.com/2073-4409/9/3/699<i>candida</i>candidalysinantimicrobial peptidealarminatpdefensin |
| spellingShingle | Jemima Ho Don N. Wickramasinghe Spyridoula-Angeliki Nikou Bernhard Hube Jonathan P. Richardson Julian R. Naglik Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells Cells <i>candida</i> candidalysin antimicrobial peptide alarmin atp defensin |
| title | Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells |
| title_full | Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells |
| title_fullStr | Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells |
| title_full_unstemmed | Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells |
| title_short | Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells |
| title_sort | candidalysin is a potent trigger of alarmin and antimicrobial peptide release in epithelial cells |
| topic | <i>candida</i> candidalysin antimicrobial peptide alarmin atp defensin |
| url | https://www.mdpi.com/2073-4409/9/3/699 |
| work_keys_str_mv | AT jemimaho candidalysinisapotenttriggerofalarminandantimicrobialpeptidereleaseinepithelialcells AT donnwickramasinghe candidalysinisapotenttriggerofalarminandantimicrobialpeptidereleaseinepithelialcells AT spyridoulaangelikinikou candidalysinisapotenttriggerofalarminandantimicrobialpeptidereleaseinepithelialcells AT bernhardhube candidalysinisapotenttriggerofalarminandantimicrobialpeptidereleaseinepithelialcells AT jonathanprichardson candidalysinisapotenttriggerofalarminandantimicrobialpeptidereleaseinepithelialcells AT julianrnaglik candidalysinisapotenttriggerofalarminandantimicrobialpeptidereleaseinepithelialcells |