3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System
Current conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here w...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/8/7/97 |
| _version_ | 1797527630830895104 |
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| author | Jennifer Rosendahl Andreas Svanström Mattias Berglin Sarunas Petronis Yalda Bogestål Patrik Stenlund Simon Standoft Anders Ståhlberg Göran Landberg Gary Chinga-Carrasco Joakim Håkansson |
| author_facet | Jennifer Rosendahl Andreas Svanström Mattias Berglin Sarunas Petronis Yalda Bogestål Patrik Stenlund Simon Standoft Anders Ståhlberg Göran Landberg Gary Chinga-Carrasco Joakim Håkansson |
| author_sort | Jennifer Rosendahl |
| collection | DOAJ |
| description | Current conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker <i>CD44</i> and the migration markers <i>VIM</i> and <i>SNAI1</i> in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker <i>CD44</i> and migration marker <i>VIM</i> which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening. |
| first_indexed | 2024-03-10T09:46:29Z |
| format | Article |
| id | doaj.art-ec6b843993de405d9503480422ef04b9 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T09:46:29Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-ec6b843993de405d9503480422ef04b92023-11-22T03:15:11ZengMDPI AGBioengineering2306-53542021-07-01879710.3390/bioengineering80700973D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model SystemJennifer Rosendahl0Andreas Svanström1Mattias Berglin2Sarunas Petronis3Yalda Bogestål4Patrik Stenlund5Simon Standoft6Anders Ståhlberg7Göran Landberg8Gary Chinga-Carrasco9Joakim Håkansson10Unit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, SwedenSahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Box 425, Medicinaregatan 1G, SE-41390 Gothenburg, SwedenUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, SwedenUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, SwedenUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, SwedenUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, SwedenUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, SwedenSahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Box 425, Medicinaregatan 1G, SE-41390 Gothenburg, SwedenSahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Box 425, Medicinaregatan 1G, SE-41390 Gothenburg, SwedenRISE PFI AS, Høgskoleringen 6b, NO-7491 Trondheim, NorwayUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, SwedenCurrent conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker <i>CD44</i> and the migration markers <i>VIM</i> and <i>SNAI1</i> in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker <i>CD44</i> and migration marker <i>VIM</i> which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening.https://www.mdpi.com/2306-5354/8/7/97nanocellulose3D printingcancer3D cell cultureCNFcancer stemness |
| spellingShingle | Jennifer Rosendahl Andreas Svanström Mattias Berglin Sarunas Petronis Yalda Bogestål Patrik Stenlund Simon Standoft Anders Ståhlberg Göran Landberg Gary Chinga-Carrasco Joakim Håkansson 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System Bioengineering nanocellulose 3D printing cancer 3D cell culture CNF cancer stemness |
| title | 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System |
| title_full | 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System |
| title_fullStr | 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System |
| title_full_unstemmed | 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System |
| title_short | 3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System |
| title_sort | 3d printed nanocellulose scaffolds as a cancer cell culture model system |
| topic | nanocellulose 3D printing cancer 3D cell culture CNF cancer stemness |
| url | https://www.mdpi.com/2306-5354/8/7/97 |
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