Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damage
Lifelong overexpression of heat shock protein (HSP) 72 in skeletal muscle is known to protect against age-related oxidative stress and muscle damage. This study aimed to ascertain whether exhaustive exercise (EE)-induced muscle fatigue and damage can be prevented by lifelong overexpression of HSP72...
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| Format: | Article |
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Elsevier
2013-01-01
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| Series: | Journal of the Formosan Medical Association |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664612001684 |
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| author | Chieh-Chung Liu Cheng-Hsien Lin Chuan-Yi Lin Ching-Cheng Lee Mao-Tsun Lin Hsiao-Chuan Wen |
| author_facet | Chieh-Chung Liu Cheng-Hsien Lin Chuan-Yi Lin Ching-Cheng Lee Mao-Tsun Lin Hsiao-Chuan Wen |
| author_sort | Chieh-Chung Liu |
| collection | DOAJ |
| description | Lifelong overexpression of heat shock protein (HSP) 72 in skeletal muscle is known to protect against age-related oxidative stress and muscle damage. This study aimed to ascertain whether exhaustive exercise (EE)-induced muscle fatigue and damage can be prevented by lifelong overexpression of HSP72 in skeletal muscle.
Methods: Transgenic mice heterozygous for the porcine HSP70.2 gene ([+]HSP72) and transgene-negative littermate controls ([–]HSP72) were subjected to an EE protocol. Mice were randomly divided into four groups: sedentary [–]HSP72, sedentary [+]HSP72, EE [–]HSP72, and EE [+]HSP72. Animals were killed 82 minutes after the start of EE to determine muscular levels of HSP72, serum levels of superoxide dismutase (SOD, an antioxidant enzyme) and lactate (an indicator of muscle fatigue), muscular levels of matrix metalloproteinase (an indicator of inflammatory myopathies), and muscular damage.
Results: During the test, the latency value for the occurrence of EE was 79–85 minutes and 100–110 minutes for [–]HSP72 and [+]HSP72 mice, respectively. After EE, [+]HSP72 mice had significantly higher serum SOD and significantly lower serum lactate, muscular matrix metalloproteinase or myeloperoxidase activity, and muscle damage compared to [–]HSP72 mice.
Conclusion: The results suggest that HSP72 overexpression in skeletal muscle may improve muscle fatigue and damage in EE by reducing oxidative damage and phagocytic infiltration, at least in mice. |
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| format | Article |
| id | doaj.art-ec6b97845443432b93a096d2f0e14620 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-11T21:56:11Z |
| publishDate | 2013-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-ec6b97845443432b93a096d2f0e146202022-12-22T00:49:19ZengElsevierJournal of the Formosan Medical Association0929-66462013-01-011121243010.1016/j.jfma.2012.02.007Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damageChieh-Chung Liu0Cheng-Hsien Lin1Chuan-Yi Lin2Ching-Cheng Lee3Mao-Tsun Lin4Hsiao-Chuan Wen5Department of Physical Education, Yuanpei University, Hsin-Chu, TaiwanSport Sciences for the Promotion of Active Life, Faculty of Human Sciences, Waseda University, Saitama, JapanDepartment of Radiological Technology, Yuanpei University, Hsinchu, TaiwanDepartment of Pathology and Laboratory Medicine, Shin-Kong Hospital, Taipei, TaiwanDepartment of Medical Research, Chi Mei Medical Center, Tainan, TaiwanDepartment of Radiological Technology, Yuanpei University, Hsinchu, TaiwanLifelong overexpression of heat shock protein (HSP) 72 in skeletal muscle is known to protect against age-related oxidative stress and muscle damage. This study aimed to ascertain whether exhaustive exercise (EE)-induced muscle fatigue and damage can be prevented by lifelong overexpression of HSP72 in skeletal muscle. Methods: Transgenic mice heterozygous for the porcine HSP70.2 gene ([+]HSP72) and transgene-negative littermate controls ([–]HSP72) were subjected to an EE protocol. Mice were randomly divided into four groups: sedentary [–]HSP72, sedentary [+]HSP72, EE [–]HSP72, and EE [+]HSP72. Animals were killed 82 minutes after the start of EE to determine muscular levels of HSP72, serum levels of superoxide dismutase (SOD, an antioxidant enzyme) and lactate (an indicator of muscle fatigue), muscular levels of matrix metalloproteinase (an indicator of inflammatory myopathies), and muscular damage. Results: During the test, the latency value for the occurrence of EE was 79–85 minutes and 100–110 minutes for [–]HSP72 and [+]HSP72 mice, respectively. After EE, [+]HSP72 mice had significantly higher serum SOD and significantly lower serum lactate, muscular matrix metalloproteinase or myeloperoxidase activity, and muscle damage compared to [–]HSP72 mice. Conclusion: The results suggest that HSP72 overexpression in skeletal muscle may improve muscle fatigue and damage in EE by reducing oxidative damage and phagocytic infiltration, at least in mice.http://www.sciencedirect.com/science/article/pii/S0929664612001684exhaustive exerciseheat shock protein 72lactatematrix metalloproteinasesuperoxide dismutase |
| spellingShingle | Chieh-Chung Liu Cheng-Hsien Lin Chuan-Yi Lin Ching-Cheng Lee Mao-Tsun Lin Hsiao-Chuan Wen Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damage Journal of the Formosan Medical Association exhaustive exercise heat shock protein 72 lactate matrix metalloproteinase superoxide dismutase |
| title | Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damage |
| title_full | Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damage |
| title_fullStr | Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damage |
| title_full_unstemmed | Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damage |
| title_short | Transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise-induced skeletal muscle damage |
| title_sort | transgenic overexpression of heat shock protein 72 in mouse muscle protects against exhaustive exercise induced skeletal muscle damage |
| topic | exhaustive exercise heat shock protein 72 lactate matrix metalloproteinase superoxide dismutase |
| url | http://www.sciencedirect.com/science/article/pii/S0929664612001684 |
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