The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle Cells
Background: Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) is one of the predominant sources of the production of free oxygen radicals in the vascular wall. Endothelin-1 is a potent vasoconstrictor factor that also has mitogenic activity in vascular smooth muscle cells. The activa...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2018-06-01
|
| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/9631 |
| _version_ | 1827843125314322432 |
|---|---|
| author | Parisa Dayati Reyhaneh Niayesh-Mehr Alireza Jafari Hossein Babaahmadi-Rezaei |
| author_facet | Parisa Dayati Reyhaneh Niayesh-Mehr Alireza Jafari Hossein Babaahmadi-Rezaei |
| author_sort | Parisa Dayati |
| collection | DOAJ |
| description | Background: Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) is one of the predominant sources of the production of free oxygen radicals in the vascular wall. Endothelin-1 is a potent vasoconstrictor factor that also has mitogenic activity in vascular smooth muscle cells. The activation of transforming growth factor beta (TGF–β) receptor by endothelin-1 plays an important role in cardiovascular diseases. The aim of this study was to investigate the changes of Nox gene expression in smooth muscle cells treated with endothelin-1 in the presence of the transforming growth factor beta-receptor antagonist.
Methods: Human aortic smooth muscle cells (HA-SMCs) were treated with endothelin-1 (100 nM) and transforming growth factor beta (2 ng/ml) in the presence and absence of transforming growth factor beta-receptor antagonist. The mRNA expression of Nox1 and Nox4 were assessed using real-time polymerase chain reaction (PCR) technique.
Findings: The gene expression of Nox1 increased in the presence of endothelin-1 compared to control group, but there was no change in gene expression of Nox4. Increasing the expression of Nox1 decreased in the presence of transforming growth factor beta-receptor antagonist (10 μM).
Conclusion: The results of this study showed that activation of transforming growth factor beta pathway is one of the mechanisms for increasing the mRNA expression of Nox1 by endothelin-1. |
| first_indexed | 2024-03-12T08:20:15Z |
| format | Article |
| id | doaj.art-ec712ae29933462fb2792fd302d09f6c |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:20:15Z |
| publishDate | 2018-06-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-ec712ae29933462fb2792fd302d09f6c2023-09-02T18:36:27ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2018-06-013647434435010.22122/jims.v36i474.96312957The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle CellsParisa Dayati0Reyhaneh Niayesh-Mehr1Alireza Jafari2Hossein Babaahmadi-Rezaei3Atherosclerosis Research Center, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranAtherosclerosis Research Center, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranAtherosclerosis Research Center, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranAssistant Professor, Atherosclerosis Research Center, Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) is one of the predominant sources of the production of free oxygen radicals in the vascular wall. Endothelin-1 is a potent vasoconstrictor factor that also has mitogenic activity in vascular smooth muscle cells. The activation of transforming growth factor beta (TGF–β) receptor by endothelin-1 plays an important role in cardiovascular diseases. The aim of this study was to investigate the changes of Nox gene expression in smooth muscle cells treated with endothelin-1 in the presence of the transforming growth factor beta-receptor antagonist. Methods: Human aortic smooth muscle cells (HA-SMCs) were treated with endothelin-1 (100 nM) and transforming growth factor beta (2 ng/ml) in the presence and absence of transforming growth factor beta-receptor antagonist. The mRNA expression of Nox1 and Nox4 were assessed using real-time polymerase chain reaction (PCR) technique. Findings: The gene expression of Nox1 increased in the presence of endothelin-1 compared to control group, but there was no change in gene expression of Nox4. Increasing the expression of Nox1 decreased in the presence of transforming growth factor beta-receptor antagonist (10 μM). Conclusion: The results of this study showed that activation of transforming growth factor beta pathway is one of the mechanisms for increasing the mRNA expression of Nox1 by endothelin-1.http://jims.mui.ac.ir/index.php/jims/article/view/9631NADPH oxidaseEndothelin-1Transforming growth factor beta |
| spellingShingle | Parisa Dayati Reyhaneh Niayesh-Mehr Alireza Jafari Hossein Babaahmadi-Rezaei The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle Cells مجله دانشکده پزشکی اصفهان NADPH oxidase Endothelin-1 Transforming growth factor beta |
| title | The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle Cells |
| title_full | The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle Cells |
| title_fullStr | The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle Cells |
| title_full_unstemmed | The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle Cells |
| title_short | The Effect of Endothelin-1 on Gene Expression of Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase via Transforming Growth Factor Beta (TGF–β) Receptor in Human Aortic Smooth Muscle Cells |
| title_sort | effect of endothelin 1 on gene expression of nicotinamide adenine dinucleotide phosphate nadph oxidase via transforming growth factor beta tgf β receptor in human aortic smooth muscle cells |
| topic | NADPH oxidase Endothelin-1 Transforming growth factor beta |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/9631 |
| work_keys_str_mv | AT parisadayati theeffectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells AT reyhanehniayeshmehr theeffectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells AT alirezajafari theeffectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells AT hosseinbabaahmadirezaei theeffectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells AT parisadayati effectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells AT reyhanehniayeshmehr effectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells AT alirezajafari effectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells AT hosseinbabaahmadirezaei effectofendothelin1ongeneexpressionofnicotinamideadeninedinucleotidephosphatenadphoxidaseviatransforminggrowthfactorbetatgfbreceptorinhumanaorticsmoothmusclecells |