<i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice
In our previous study, <i>Lactiplantibacillus plantarum</i> Y42 showed some potential probiotic functions and the ability to form biofilm. The aim of this study was to compare the similarities and differences in the probiotic and physiological traits of <i>L. plantarum</i> Y4...
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MDPI AG
2022-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/10/1451 |
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| author | Lijuan Zhang Yuan Meng Jiayi Li Jiang Yu Guangqing Mu Yanfeng Tuo |
| author_facet | Lijuan Zhang Yuan Meng Jiayi Li Jiang Yu Guangqing Mu Yanfeng Tuo |
| author_sort | Lijuan Zhang |
| collection | DOAJ |
| description | In our previous study, <i>Lactiplantibacillus plantarum</i> Y42 showed some potential probiotic functions and the ability to form biofilm. The aim of this study was to compare the similarities and differences in the probiotic and physiological traits of <i>L. plantarum</i> Y42 in the biofilm and planktonic states. <i>L. plantarum</i> Y42 in the biofilm state was proven to have higher survival after passing through mimic gastrointestinal fluid, as well as excellent adhesion properties on the HT-29 cell monolayers, than those in the planktonic state. The expression of tight junction proteins (TJ proteins) of HT-29 cell monolayers treated by <i>L. plantarum</i> Y42 in the planktonic state increased, while similar changes were not observed in the HT-29 cells treated by the strain in the biofilm state. Furthermore, Balb/c mice were orally administered <i>L. plantarum</i> Y42 in the biofilm and planktonic states, respectively. Compared to the planktonic state, the oral administration of <i>L. plantarum</i> Y42 in the biofilm state significantly boosted IgA levels and improved the immunity of the mice. High-throughput sequencing showed that the diversity and structure of the intestinal flora of the mice were changed after the oral administration of <i>L. plantarum</i> Y42, including the up-regulated relative abundance of <i>Lactobacillus</i> in the intestinal tract of the mice, with no difference between the biofilm and planktonic states. Moreover, oral administration of <i>L. plantarum</i> Y42 in biofilm and planktonic states reduced the release of proinflammatory factors, to a certain extent, in the serum of the mice. The similarities and differences in the probiotic and physiological properties of <i>L. plantarum</i> Y42 in the biofilm and planktonic states can be contributed to the reasonable application of the strain. |
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| spelling | doaj.art-ec71c4ff870d4f0489a29496bd7f1de62023-11-23T10:59:20ZengMDPI AGFoods2304-81582022-05-011110145110.3390/foods11101451<i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c MiceLijuan Zhang0Yuan Meng1Jiayi Li2Jiang Yu3Guangqing Mu4Yanfeng Tuo5School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaDalian Probiotics Function Research Key Laboratory, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaIn our previous study, <i>Lactiplantibacillus plantarum</i> Y42 showed some potential probiotic functions and the ability to form biofilm. The aim of this study was to compare the similarities and differences in the probiotic and physiological traits of <i>L. plantarum</i> Y42 in the biofilm and planktonic states. <i>L. plantarum</i> Y42 in the biofilm state was proven to have higher survival after passing through mimic gastrointestinal fluid, as well as excellent adhesion properties on the HT-29 cell monolayers, than those in the planktonic state. The expression of tight junction proteins (TJ proteins) of HT-29 cell monolayers treated by <i>L. plantarum</i> Y42 in the planktonic state increased, while similar changes were not observed in the HT-29 cells treated by the strain in the biofilm state. Furthermore, Balb/c mice were orally administered <i>L. plantarum</i> Y42 in the biofilm and planktonic states, respectively. Compared to the planktonic state, the oral administration of <i>L. plantarum</i> Y42 in the biofilm state significantly boosted IgA levels and improved the immunity of the mice. High-throughput sequencing showed that the diversity and structure of the intestinal flora of the mice were changed after the oral administration of <i>L. plantarum</i> Y42, including the up-regulated relative abundance of <i>Lactobacillus</i> in the intestinal tract of the mice, with no difference between the biofilm and planktonic states. Moreover, oral administration of <i>L. plantarum</i> Y42 in biofilm and planktonic states reduced the release of proinflammatory factors, to a certain extent, in the serum of the mice. The similarities and differences in the probiotic and physiological properties of <i>L. plantarum</i> Y42 in the biofilm and planktonic states can be contributed to the reasonable application of the strain.https://www.mdpi.com/2304-8158/11/10/1451biofilmplanktonic<i>Lactobacillus plantarum</i>intestinal barriergut flora |
| spellingShingle | Lijuan Zhang Yuan Meng Jiayi Li Jiang Yu Guangqing Mu Yanfeng Tuo <i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice Foods biofilm planktonic <i>Lactobacillus plantarum</i> intestinal barrier gut flora |
| title | <i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice |
| title_full | <i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice |
| title_fullStr | <i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice |
| title_full_unstemmed | <i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice |
| title_short | <i>Lactiplantibacillus plantarum</i> Y42 in Biofilm and Planktonic States Improves Intestinal Barrier Integrity and Modulates Gut Microbiota of Balb/c Mice |
| title_sort | i lactiplantibacillus plantarum i y42 in biofilm and planktonic states improves intestinal barrier integrity and modulates gut microbiota of balb c mice |
| topic | biofilm planktonic <i>Lactobacillus plantarum</i> intestinal barrier gut flora |
| url | https://www.mdpi.com/2304-8158/11/10/1451 |
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