Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP)
Abstract Background Obesity is associated with the development and progression of osteoarthritis (OA). Although the infrapatellar fat pad (IFP) could be involved in this association, due to its intracapsular localization in the knee joint, there is currently little known about the effect of obesity...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Arthritis Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13075-017-1395-9 |
| _version_ | 1818497723327840256 |
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| author | Anja J. de Jong Inge R. Klein-Wieringa Stefan N. Andersen Joanneke C. Kwekkeboom Linda Herb-van Toorn Badelog J. E. de Lange-Brokaar Danny van Delft John Garcia Wu Wei Huub J. L. van der Heide Yvonne M. Bastiaansen-Jenniskens Gerjo J. V. M. van Osch Annemarie M. Zuurmond Vedrana Stojanovic-Susulic Rob G. H. H. Nelissen René E. M. Toes Margreet Kloppenburg Andreea Ioan-Facsinay |
| author_facet | Anja J. de Jong Inge R. Klein-Wieringa Stefan N. Andersen Joanneke C. Kwekkeboom Linda Herb-van Toorn Badelog J. E. de Lange-Brokaar Danny van Delft John Garcia Wu Wei Huub J. L. van der Heide Yvonne M. Bastiaansen-Jenniskens Gerjo J. V. M. van Osch Annemarie M. Zuurmond Vedrana Stojanovic-Susulic Rob G. H. H. Nelissen René E. M. Toes Margreet Kloppenburg Andreea Ioan-Facsinay |
| author_sort | Anja J. de Jong |
| collection | DOAJ |
| description | Abstract Background Obesity is associated with the development and progression of osteoarthritis (OA). Although the infrapatellar fat pad (IFP) could be involved in this association, due to its intracapsular localization in the knee joint, there is currently little known about the effect of obesity on the IFP. Therefore, we investigated cellular and molecular body mass index (BMI)-related features in the IFP of OA patients. Methods Patients with knee OA (N = 155, 68% women, mean age 65 years, mean (SD) BMI 29.9 kg/m2 (5.7)) were recruited: IFP volume was determined by magnetic resonance imaging in 79 patients with knee OA, while IFPs and subcutaneous adipose tissue (SCAT) were obtained from 106 patients undergoing arthroplasty. Crown-like structures (CLS) were determined using immunohistochemical analysis. Adipocyte size was determined by light microscopy and histological analysis. Stromal vascular fraction (SVF) cells were characterized by flow cytometry. Results IFP volume (mean (SD) 23.6 (5.4) mm3) was associated with height, but not with BMI or other obesity-related features. Likewise, volume and size of IFP adipocytes (mean 271 pl, mean 1933 μm) was not correlated with BMI. Few CLS were observed in the IFP, with no differences between overweight/obese and lean individuals. Moreover, high BMI was not associated with higher SVF immune cell numbers in the IFP, nor with changes in their phenotype. No BMI-associated molecular differences were observed, besides an increase in TNFα expression with high BMI. Macrophages in the IFP were mostly pro-inflammatory, producing IL-6 and TNFα, but little IL-10. Interestingly, however, CD206 and CD163 were associated with an anti-inflammatory phenotype, were the most abundantly expressed surface markers on macrophages (81% and 41%, respectively) and CD163+ macrophages had a more activated and pro-inflammatory phenotype than their CD163- counterparts. Conclusions BMI-related features usually observed in SCAT and visceral adipose tissue could not be detected in the IFP of OA patients, a fat depot implicated in OA pathogenesis. |
| first_indexed | 2024-12-10T18:48:50Z |
| format | Article |
| id | doaj.art-ec74ab8669fe45e4a28ab17c323dcccf |
| institution | Directory Open Access Journal |
| issn | 1478-6362 |
| language | English |
| last_indexed | 2024-12-10T18:48:50Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Arthritis Research & Therapy |
| spelling | doaj.art-ec74ab8669fe45e4a28ab17c323dcccf2022-12-22T01:37:23ZengBMCArthritis Research & Therapy1478-63622017-08-0119111210.1186/s13075-017-1395-9Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP)Anja J. de Jong0Inge R. Klein-Wieringa1Stefan N. Andersen2Joanneke C. Kwekkeboom3Linda Herb-van Toorn4Badelog J. E. de Lange-Brokaar5Danny van Delft6John Garcia7Wu Wei8Huub J. L. van der Heide9Yvonne M. Bastiaansen-Jenniskens10Gerjo J. V. M. van Osch11Annemarie M. Zuurmond12Vedrana Stojanovic-Susulic13Rob G. H. H. Nelissen14René E. M. Toes15Margreet Kloppenburg16Andreea Ioan-Facsinay17Department of Rheumatology, Leiden University Medical CentreDepartment of Rheumatology, Leiden University Medical CentreDepartment of Rheumatology, Leiden University Medical CentreDepartment of Rheumatology, Leiden University Medical CentreDepartment of Rheumatology, Leiden University Medical CentreDepartment of Rheumatology, Leiden University Medical CentreDepartment of Orthopaedics, Leiden University Medical CenterDepartment of Orthopaedics, Erasmus MC, University Medical CenterDepartment of Orthopaedics, Erasmus MC, University Medical CenterDepartment of Orthopaedics, Leiden University Medical CenterDepartment of Orthopaedics, Erasmus MC, University Medical CenterDepartment of Orthopaedics, Erasmus MC, University Medical CenterTNOJanssen, Pharmaceutical Companies Johnson & JohnsonDepartment of Orthopaedics, Leiden University Medical CenterDepartment of Rheumatology, Leiden University Medical CentreDepartment of Rheumatology, Leiden University Medical CentreDepartment of Rheumatology, Leiden University Medical CentreAbstract Background Obesity is associated with the development and progression of osteoarthritis (OA). Although the infrapatellar fat pad (IFP) could be involved in this association, due to its intracapsular localization in the knee joint, there is currently little known about the effect of obesity on the IFP. Therefore, we investigated cellular and molecular body mass index (BMI)-related features in the IFP of OA patients. Methods Patients with knee OA (N = 155, 68% women, mean age 65 years, mean (SD) BMI 29.9 kg/m2 (5.7)) were recruited: IFP volume was determined by magnetic resonance imaging in 79 patients with knee OA, while IFPs and subcutaneous adipose tissue (SCAT) were obtained from 106 patients undergoing arthroplasty. Crown-like structures (CLS) were determined using immunohistochemical analysis. Adipocyte size was determined by light microscopy and histological analysis. Stromal vascular fraction (SVF) cells were characterized by flow cytometry. Results IFP volume (mean (SD) 23.6 (5.4) mm3) was associated with height, but not with BMI or other obesity-related features. Likewise, volume and size of IFP adipocytes (mean 271 pl, mean 1933 μm) was not correlated with BMI. Few CLS were observed in the IFP, with no differences between overweight/obese and lean individuals. Moreover, high BMI was not associated with higher SVF immune cell numbers in the IFP, nor with changes in their phenotype. No BMI-associated molecular differences were observed, besides an increase in TNFα expression with high BMI. Macrophages in the IFP were mostly pro-inflammatory, producing IL-6 and TNFα, but little IL-10. Interestingly, however, CD206 and CD163 were associated with an anti-inflammatory phenotype, were the most abundantly expressed surface markers on macrophages (81% and 41%, respectively) and CD163+ macrophages had a more activated and pro-inflammatory phenotype than their CD163- counterparts. Conclusions BMI-related features usually observed in SCAT and visceral adipose tissue could not be detected in the IFP of OA patients, a fat depot implicated in OA pathogenesis.http://link.springer.com/article/10.1186/s13075-017-1395-9OsteoarthritisInfrapatellar fat padObesityInflammationMacrophages |
| spellingShingle | Anja J. de Jong Inge R. Klein-Wieringa Stefan N. Andersen Joanneke C. Kwekkeboom Linda Herb-van Toorn Badelog J. E. de Lange-Brokaar Danny van Delft John Garcia Wu Wei Huub J. L. van der Heide Yvonne M. Bastiaansen-Jenniskens Gerjo J. V. M. van Osch Annemarie M. Zuurmond Vedrana Stojanovic-Susulic Rob G. H. H. Nelissen René E. M. Toes Margreet Kloppenburg Andreea Ioan-Facsinay Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP) Arthritis Research & Therapy Osteoarthritis Infrapatellar fat pad Obesity Inflammation Macrophages |
| title | Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP) |
| title_full | Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP) |
| title_fullStr | Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP) |
| title_full_unstemmed | Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP) |
| title_short | Lack of high BMI-related features in adipocytes and inflammatory cells in the infrapatellar fat pad (IFP) |
| title_sort | lack of high bmi related features in adipocytes and inflammatory cells in the infrapatellar fat pad ifp |
| topic | Osteoarthritis Infrapatellar fat pad Obesity Inflammation Macrophages |
| url | http://link.springer.com/article/10.1186/s13075-017-1395-9 |
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