Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity
Naringin is a dietary flavonoid glycoside with broad bioactivities, and it has been found to undergo extensive microbial metabolism in human gut. Microbial metabolites are believed to play an important role in the overall bioactivity of naringin. However, knowledge is scarce about its microbial meta...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/18/3765 |
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| author | Xuan Zeng Yuying Zheng Yan He Jiashuo Zhang Wei Peng Weiwei Su |
| author_facet | Xuan Zeng Yuying Zheng Yan He Jiashuo Zhang Wei Peng Weiwei Su |
| author_sort | Xuan Zeng |
| collection | DOAJ |
| description | Naringin is a dietary flavonoid glycoside with broad bioactivities, and it has been found to undergo extensive microbial metabolism in human gut. Microbial metabolites are believed to play an important role in the overall bioactivity of naringin. However, knowledge is scarce about its microbial metabolism in laboratory rats, which are the most commonly used animal model for naringin-related biomedical studies. Herein, we profiled the microbial metabolism of naringin in rat by an in vitro anaerobic fermentation combined with LC-MS/MS methods. A total of 35 microbial metabolites were identified, and corresponding metabolic pathways were proposed. Naringin and its metabolites were further quantified in fermentation samples. Rhoifolin, neoeriocitrin, neohesperidin, naringenin, methylated naringin, and hydroxylated naringin were detected as the primary microbial metabolites. Moreover, antioxidant capacity assays suggested that fermentation-associated microbial metabolites exhibited higher antioxidant activity than original naringin. Obtained results contribute to a more comprehensive understanding of the microbial metabolism and antioxidant capacity of naringin. |
| first_indexed | 2024-03-09T22:55:39Z |
| format | Article |
| id | doaj.art-ec75dbc6e0604b488d0e7034d3a2d3c5 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T22:55:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ec75dbc6e0604b488d0e7034d3a2d3c52023-11-23T18:11:27ZengMDPI AGNutrients2072-66432022-09-011418376510.3390/nu14183765Microbial Metabolism of Naringin and the Impact on Antioxidant CapacityXuan Zeng0Yuying Zheng1Yan He2Jiashuo Zhang3Wei Peng4Weiwei Su5Guangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaGuangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaNaringin is a dietary flavonoid glycoside with broad bioactivities, and it has been found to undergo extensive microbial metabolism in human gut. Microbial metabolites are believed to play an important role in the overall bioactivity of naringin. However, knowledge is scarce about its microbial metabolism in laboratory rats, which are the most commonly used animal model for naringin-related biomedical studies. Herein, we profiled the microbial metabolism of naringin in rat by an in vitro anaerobic fermentation combined with LC-MS/MS methods. A total of 35 microbial metabolites were identified, and corresponding metabolic pathways were proposed. Naringin and its metabolites were further quantified in fermentation samples. Rhoifolin, neoeriocitrin, neohesperidin, naringenin, methylated naringin, and hydroxylated naringin were detected as the primary microbial metabolites. Moreover, antioxidant capacity assays suggested that fermentation-associated microbial metabolites exhibited higher antioxidant activity than original naringin. Obtained results contribute to a more comprehensive understanding of the microbial metabolism and antioxidant capacity of naringin.https://www.mdpi.com/2072-6643/14/18/3765naringinrat microbiotametabolismantioxidant capacity |
| spellingShingle | Xuan Zeng Yuying Zheng Yan He Jiashuo Zhang Wei Peng Weiwei Su Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity Nutrients naringin rat microbiota metabolism antioxidant capacity |
| title | Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity |
| title_full | Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity |
| title_fullStr | Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity |
| title_full_unstemmed | Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity |
| title_short | Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity |
| title_sort | microbial metabolism of naringin and the impact on antioxidant capacity |
| topic | naringin rat microbiota metabolism antioxidant capacity |
| url | https://www.mdpi.com/2072-6643/14/18/3765 |
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