Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal Samples
In the present study, we aimed to determine the antimicrobial resistance and molecular typing of <i>Staphylococcus aureus</i> recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 <i>S. a...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/11/2143 |
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| author | Renata P. Santos Fernando N. Souza Ana Claudia D. Oliveira Antônio F. de Souza Filho Juliana Aizawa Luisa Z. Moreno Adriano F. da Cunha Adriana Cortez Alice M.M.P. Della Libera Marcos B. Heinemann Mônica M.O.P. Cerqueira |
| author_facet | Renata P. Santos Fernando N. Souza Ana Claudia D. Oliveira Antônio F. de Souza Filho Juliana Aizawa Luisa Z. Moreno Adriano F. da Cunha Adriana Cortez Alice M.M.P. Della Libera Marcos B. Heinemann Mônica M.O.P. Cerqueira |
| author_sort | Renata P. Santos |
| collection | DOAJ |
| description | In the present study, we aimed to determine the antimicrobial resistance and molecular typing of <i>Staphylococcus aureus</i> recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 <i>S. aureus</i> strains using a broad antimicrobial susceptibility profile. Furthermore, 107 <i>S. aureus</i> isolates were strain-typed using staphylococcal protein-A (spa) typing. A large proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicillin-resistant <i>S. aureus</i> strains were found. Our study did not strengthen the idea that extramammary niches (i.e., nares/muzzles) are an important source of <i>S. aureus</i> for bovine mastitis. A discrepancy in the antimicrobial resistance between <i>S. aureus</i> strains isolated from nares/muzzles and milk samples was observed. Furthermore, <i>S. aureus</i> isolates from transient and persistent intramammary infections (IMIs) did not differ by spa typing, suggesting that the persistence of bovine IMIs was determined by cow factors. Thus, the high level of multidrug-resistant <i>S. aureus</i> found in the two herds, considered together with the predominance of a well udder-adapted <i>S. aureus</i> strain, may contribute to our knowledge of the history of the high prevalence of mastitis caused by <i>S. aureus</i>, which is of great concern for animal and public health. |
| first_indexed | 2024-03-10T14:46:18Z |
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| id | doaj.art-ec76f9d5647146a0989ad66ea7aa01f1 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T14:46:18Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ec76f9d5647146a0989ad66ea7aa01f12023-11-20T21:22:26ZengMDPI AGAnimals2076-26152020-11-011011214310.3390/ani10112143Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal SamplesRenata P. Santos0Fernando N. Souza1Ana Claudia D. Oliveira2Antônio F. de Souza Filho3Juliana Aizawa4Luisa Z. Moreno5Adriano F. da Cunha6Adriana Cortez7Alice M.M.P. Della Libera8Marcos B. Heinemann9Mônica M.O.P. Cerqueira10Departamento de Inspeção e Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, BrazilDepartamento de Clínica Médica, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, BrazilDepartamento de Inspeção e Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, BrazilDepartamento de Inspeção e Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, BrazilCurso de Medicina Veterinária, Universidade Santo Amaro, Rua Prof. Enéas de Siqueira Neto 340, São Paulo 04829-300, BrazilDepartamento de Clínica Médica, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, BrazilDepartamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina e Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo 05508-270, BrazilDepartamento de Inspeção e Produtos de Origem Animal, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte 31270-901, BrazilIn the present study, we aimed to determine the antimicrobial resistance and molecular typing of <i>Staphylococcus aureus</i> recovered from transient and persistent intramammary infections and nares/muzzles in dairy cows. We investigated the antimicrobial resistance of 189 <i>S. aureus</i> strains using a broad antimicrobial susceptibility profile. Furthermore, 107 <i>S. aureus</i> isolates were strain-typed using staphylococcal protein-A (spa) typing. A large proportion of strains exhibited multidrug resistance to antimicrobials, including resistance to critically important antimicrobials, although no methicillin-resistant <i>S. aureus</i> strains were found. Our study did not strengthen the idea that extramammary niches (i.e., nares/muzzles) are an important source of <i>S. aureus</i> for bovine mastitis. A discrepancy in the antimicrobial resistance between <i>S. aureus</i> strains isolated from nares/muzzles and milk samples was observed. Furthermore, <i>S. aureus</i> isolates from transient and persistent intramammary infections (IMIs) did not differ by spa typing, suggesting that the persistence of bovine IMIs was determined by cow factors. Thus, the high level of multidrug-resistant <i>S. aureus</i> found in the two herds, considered together with the predominance of a well udder-adapted <i>S. aureus</i> strain, may contribute to our knowledge of the history of the high prevalence of mastitis caused by <i>S. aureus</i>, which is of great concern for animal and public health.https://www.mdpi.com/2076-2615/10/11/2143intramammary infectionspa typingstaphylococciantimicrobial susceptibilitydairy cow |
| spellingShingle | Renata P. Santos Fernando N. Souza Ana Claudia D. Oliveira Antônio F. de Souza Filho Juliana Aizawa Luisa Z. Moreno Adriano F. da Cunha Adriana Cortez Alice M.M.P. Della Libera Marcos B. Heinemann Mônica M.O.P. Cerqueira Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal Samples Animals intramammary infection spa typing staphylococci antimicrobial susceptibility dairy cow |
| title | Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal Samples |
| title_full | Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal Samples |
| title_fullStr | Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal Samples |
| title_full_unstemmed | Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal Samples |
| title_short | Molecular Typing and Antimicrobial Susceptibility Profile of <i>Staphylococcus aureus</i> Isolates Recovered from Bovine Mastitis and Nasal Samples |
| title_sort | molecular typing and antimicrobial susceptibility profile of i staphylococcus aureus i isolates recovered from bovine mastitis and nasal samples |
| topic | intramammary infection spa typing staphylococci antimicrobial susceptibility dairy cow |
| url | https://www.mdpi.com/2076-2615/10/11/2143 |
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