| Summary: | Objective: Phytoestrogens plant compounds with biologic-estrogenic activity, structurally similar to 17-β
estradiol, are first converted to heterocyclic compounds similar to estrogens in structure and then
conjugated in the liver. Olive (Olea europaea), from the oleaseae family, is known as a phytoestrogen
plant compound since it contains lignans and phenolic compounds. The aim of the present study was to
evaluate the effect of O. europaea extract on male rats’ reproductive parameters.
Materials and Methods: In this experimental study, 40 male Wistar rats with the average weight of
200-250 g and age of 8-10 weeks, divided into 5 groups. Group 1, which called control group, received no
treatment. Group 2 received normal saline and Groups 3-5 received extract at a dose of 50, 100 and 200
mg/kg/day of the olive leaves extract (1 cc), respectively. Before the administration of the first gavage
and 24 h after that of the last one (i.e., in the 49th day), all the rats were weighed, and blood samples were
taken from their tail vein. The blood samples were then centrifuged at 1500 rpm for 20 min, then serum
was separated and stored at −80 °C for the measurement of estradiol and testosterone, using
immunoassay technique.
Results: The results show a significant decrease in testosterone and estradiol level among the five groups,
which is dependent on the concentration of the extract; the decrease in testosterone and estradiol is
positively correlated to the concentration of the extract.
Conclusion: In conclusion, olive leaves extract significantly decreased fertility parameters in the male
adult rat dose-dependently.
|
|---|