Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes
Our study explored the impact of hypergravity on human T cells, which experience additional acceleration forces beyond Earth’s gravity due to various factors, such as pulsatile blood flow, and technology, such as high-performance aircraft flights or spaceflights. We investigated the histone modifica...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/24/17232 |
| _version_ | 1797380799225397248 |
|---|---|
| author | Kendra Wernlé Cora S. Thiel Oliver Ullrich |
| author_facet | Kendra Wernlé Cora S. Thiel Oliver Ullrich |
| author_sort | Kendra Wernlé |
| collection | DOAJ |
| description | Our study explored the impact of hypergravity on human T cells, which experience additional acceleration forces beyond Earth’s gravity due to various factors, such as pulsatile blood flow, and technology, such as high-performance aircraft flights or spaceflights. We investigated the histone modifications Histone 3 lysine 4 and 9 trimethylation (H3K4me3 and H3K9me3, respectively), as well as the structural and cytoskeletal organization of Jurkat T cells in response to hypergravity. Histone modifications play a crucial role in gene regulation, chromatin organization and DNA repair. In response to hypergravity, we found only minimal changes of H3K4me3 and a rapid increase in H3K9me3, which was sustained for up to 15 min and then returned to control levels after 1 h. Furthermore, rapid changes in F-actin fluorescence were observed within seconds of hypergravity exposure, indicating filament depolymerization and cytoskeletal restructuring, which subsequently recovered after 1 h of hypergravity. Our study demonstrated the rapid, dynamic and adaptive cellular response to hypergravity, particularly in terms of histone modifications and cytoskeletal changes. These responses are likely necessary for maintaining genome stability and structural integrity under hypergravity conditions as they are constantly occurring in the human body during blood cell circulation. |
| first_indexed | 2024-03-08T20:42:17Z |
| format | Article |
| id | doaj.art-ec7983caf67546aca7dca6babcd7a202 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:42:17Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ec7983caf67546aca7dca6babcd7a2022023-12-22T14:13:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241723210.3390/ijms242417232Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T LymphocytesKendra Wernlé0Cora S. Thiel1Oliver Ullrich2Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, SwitzerlandInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, SwitzerlandInstitute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, SwitzerlandOur study explored the impact of hypergravity on human T cells, which experience additional acceleration forces beyond Earth’s gravity due to various factors, such as pulsatile blood flow, and technology, such as high-performance aircraft flights or spaceflights. We investigated the histone modifications Histone 3 lysine 4 and 9 trimethylation (H3K4me3 and H3K9me3, respectively), as well as the structural and cytoskeletal organization of Jurkat T cells in response to hypergravity. Histone modifications play a crucial role in gene regulation, chromatin organization and DNA repair. In response to hypergravity, we found only minimal changes of H3K4me3 and a rapid increase in H3K9me3, which was sustained for up to 15 min and then returned to control levels after 1 h. Furthermore, rapid changes in F-actin fluorescence were observed within seconds of hypergravity exposure, indicating filament depolymerization and cytoskeletal restructuring, which subsequently recovered after 1 h of hypergravity. Our study demonstrated the rapid, dynamic and adaptive cellular response to hypergravity, particularly in terms of histone modifications and cytoskeletal changes. These responses are likely necessary for maintaining genome stability and structural integrity under hypergravity conditions as they are constantly occurring in the human body during blood cell circulation.https://www.mdpi.com/1422-0067/24/24/17232hypergravityimmune systemhistone modificationsnuclear architecture |
| spellingShingle | Kendra Wernlé Cora S. Thiel Oliver Ullrich Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes International Journal of Molecular Sciences hypergravity immune system histone modifications nuclear architecture |
| title | Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes |
| title_full | Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes |
| title_fullStr | Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes |
| title_full_unstemmed | Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes |
| title_short | Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes |
| title_sort | increased h3k9me3 and f actin reorganization in the rapid adaptive response to hypergravity in human t lymphocytes |
| topic | hypergravity immune system histone modifications nuclear architecture |
| url | https://www.mdpi.com/1422-0067/24/24/17232 |
| work_keys_str_mv | AT kendrawernle increasedh3k9me3andfactinreorganizationintherapidadaptiveresponsetohypergravityinhumantlymphocytes AT corasthiel increasedh3k9me3andfactinreorganizationintherapidadaptiveresponsetohypergravityinhumantlymphocytes AT oliverullrich increasedh3k9me3andfactinreorganizationintherapidadaptiveresponsetohypergravityinhumantlymphocytes |