A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis
Abstract We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2, from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by t...
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Sociedade Brasileira de Genética
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400649&lng=en&tlng=en |
| _version_ | 1828426740150566912 |
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| author | Lu Zhang Weijuan Hu Yike Gao Huitang Pan Qixiang Zhang |
| author_facet | Lu Zhang Weijuan Hu Yike Gao Huitang Pan Qixiang Zhang |
| author_sort | Lu Zhang |
| collection | DOAJ |
| description | Abstract We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2, from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expression of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana constitutively expressing PfHSP17.2 displayed increased thermotolerance and higher resistance to salt and cold compared with wild type plants. It is suggested that PfHSP17.2 plays a key role in heat and other abiotic stresses. |
| first_indexed | 2024-12-10T16:49:49Z |
| format | Article |
| id | doaj.art-ec7a8135f38244b5bcbd97e00682df35 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-10T16:49:49Z |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-ec7a8135f38244b5bcbd97e00682df352022-12-22T01:40:55ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-468541364966010.1590/1678-4685-gmb-2017-0206S1415-47572018000400649A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic ArabidopsisLu ZhangWeijuan HuYike GaoHuitang PanQixiang ZhangAbstract We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2, from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expression of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana constitutively expressing PfHSP17.2 displayed increased thermotolerance and higher resistance to salt and cold compared with wild type plants. It is suggested that PfHSP17.2 plays a key role in heat and other abiotic stresses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400649&lng=en&tlng=ensHSPsstress toleranceArabidopsis |
| spellingShingle | Lu Zhang Weijuan Hu Yike Gao Huitang Pan Qixiang Zhang A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis Genetics and Molecular Biology sHSPs stress tolerance Arabidopsis |
| title | A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis |
| title_full | A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis |
| title_fullStr | A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis |
| title_full_unstemmed | A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis |
| title_short | A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis |
| title_sort | cytosolic class ii small heat shock protein pfhsp17 2 confers resistance to heat cold and salt stresses in transgenic arabidopsis |
| topic | sHSPs stress tolerance Arabidopsis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400649&lng=en&tlng=en |
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