Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness
Abstract Background Aedes aegypti is a major insect vector because it transmits dreadful viruses as adults that cause disease in humans and other vertebrates. The use of mosquito’s microbiota has shown great potential impacts on vector control and mosquito reproductive competence. The present study...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-10-01
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| Series: | Journal of Basic and Applied Zoology |
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| Online Access: | https://doi.org/10.1186/s41936-021-00246-7 |
| _version_ | 1818987271224819712 |
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| author | Gopalan Rajagopal Sakkanan Ilango |
| author_facet | Gopalan Rajagopal Sakkanan Ilango |
| author_sort | Gopalan Rajagopal |
| collection | DOAJ |
| description | Abstract Background Aedes aegypti is a major insect vector because it transmits dreadful viruses as adults that cause disease in humans and other vertebrates. The use of mosquito’s microbiota has shown great potential impacts on vector control and mosquito reproductive competence. The present study aimed to examine the resident bacteria of mosquitoes which are used as a potent range to reduce the A. aegypti fitness. Isolated resident-bacterial strains from blood-fed Aedes species were characterized using gene sequencing and phylogenetic analysis, to assess the inhabitant bacterial strains survival rate in A. aegypti midgut, instar developmental duration, malformation and reproductive competence. Results The genetic distinctiveness of isolated bacterial strains belong to the genus Exiguobacterium spp. and further non-redundant nucleotide database search revealed that the species of effective strains were E. aestuarii (MN629357) and E. profundum (MN625885). Exposure of the freshly hatched larvae with these bacteria cell densities extended the developmental duration. For instance, exposure of A. aegypti larva with 0.42 × 108, 0.84 × 108 and 1.68 × 108 cells/mL of E. aestuarii extended the total developmental duration to 11.41, 14.29 and 14.78 days, respectively. It also reduced the fecundity and hatchability of A. aegypti female, with exposure to these bacteria, from 1033.33 eggs/10 females in the control series to 656.67 eggs/10 females. Conclusions These present findings indicate that the resident-bacterial strains from blood-fed mosquito not only extend the larval durations but also rendered the A. aegypti females sterile to various extents. |
| first_indexed | 2024-12-20T19:04:02Z |
| format | Article |
| id | doaj.art-ec7bcdc17808424ea022e0e099ec9967 |
| institution | Directory Open Access Journal |
| issn | 2090-990X |
| language | English |
| last_indexed | 2024-12-20T19:04:02Z |
| publishDate | 2021-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj.art-ec7bcdc17808424ea022e0e099ec99672022-12-21T19:29:20ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2021-10-018211910.1186/s41936-021-00246-7Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitnessGopalan Rajagopal0Sakkanan Ilango1Postgraduate and Research Department of Zoology, Ayya Nadar Janaki Ammal CollegePostgraduate and Research Department of Zoology, Ayya Nadar Janaki Ammal CollegeAbstract Background Aedes aegypti is a major insect vector because it transmits dreadful viruses as adults that cause disease in humans and other vertebrates. The use of mosquito’s microbiota has shown great potential impacts on vector control and mosquito reproductive competence. The present study aimed to examine the resident bacteria of mosquitoes which are used as a potent range to reduce the A. aegypti fitness. Isolated resident-bacterial strains from blood-fed Aedes species were characterized using gene sequencing and phylogenetic analysis, to assess the inhabitant bacterial strains survival rate in A. aegypti midgut, instar developmental duration, malformation and reproductive competence. Results The genetic distinctiveness of isolated bacterial strains belong to the genus Exiguobacterium spp. and further non-redundant nucleotide database search revealed that the species of effective strains were E. aestuarii (MN629357) and E. profundum (MN625885). Exposure of the freshly hatched larvae with these bacteria cell densities extended the developmental duration. For instance, exposure of A. aegypti larva with 0.42 × 108, 0.84 × 108 and 1.68 × 108 cells/mL of E. aestuarii extended the total developmental duration to 11.41, 14.29 and 14.78 days, respectively. It also reduced the fecundity and hatchability of A. aegypti female, with exposure to these bacteria, from 1033.33 eggs/10 females in the control series to 656.67 eggs/10 females. Conclusions These present findings indicate that the resident-bacterial strains from blood-fed mosquito not only extend the larval durations but also rendered the A. aegypti females sterile to various extents.https://doi.org/10.1186/s41936-021-00246-7Inhabitant bacteriaBiocontrol agentsMosquitoDevelopmental duration and reproductive competence |
| spellingShingle | Gopalan Rajagopal Sakkanan Ilango Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness Journal of Basic and Applied Zoology Inhabitant bacteria Biocontrol agents Mosquito Developmental duration and reproductive competence |
| title | Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness |
| title_full | Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness |
| title_fullStr | Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness |
| title_full_unstemmed | Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness |
| title_short | Exposure of Exiguobacterium spp. to dengue vector, Aedes aegypti reduces growth and reproductive fitness |
| title_sort | exposure of exiguobacterium spp to dengue vector aedes aegypti reduces growth and reproductive fitness |
| topic | Inhabitant bacteria Biocontrol agents Mosquito Developmental duration and reproductive competence |
| url | https://doi.org/10.1186/s41936-021-00246-7 |
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