Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application
Corynebacterium glutamicum, a soil-derived gram-positive actinobacterium, has been widely used for the production of biochemical molecules such as amino acids (i.e., L-glutamate and L-lysine), nucleic acids, alcohols, and organic acids. The metabolism of the bacterium has been engineered to increase...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02523/full |
| _version_ | 1818427697609572352 |
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| author | Min Ju Lee Pil Kim |
| author_facet | Min Ju Lee Pil Kim |
| author_sort | Min Ju Lee |
| collection | DOAJ |
| description | Corynebacterium glutamicum, a soil-derived gram-positive actinobacterium, has been widely used for the production of biochemical molecules such as amino acids (i.e., L-glutamate and L-lysine), nucleic acids, alcohols, and organic acids. The metabolism of the bacterium has been engineered to increase the production of the target biochemical molecule, which requires a cytosolic enzyme expression. As recent demand for new proteinaceous biologics (such as antibodies, growth factors, and hormones) increase, C. glutamicum is attracting industrial interest as a recombinant protein expression host for therapeutic protein production due to the advantages such as low protease activity without endotoxin activity. In this review, we have summarized the recent studies on the heterologous expression of the recombinant protein in C. glutamicum for metabolic engineering, expansion of substrate availability, and recombinant protein secretion. We have also outlined the advances in genetic components such as promoters, surface anchoring systems, and secretory signal sequences in C. glutamicum for effective recombinant protein expression. |
| first_indexed | 2024-12-14T14:49:51Z |
| format | Article |
| id | doaj.art-ec7bf86ec1994ad8b7da1cc55509a8e2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T14:49:51Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ec7bf86ec1994ad8b7da1cc55509a8e22022-12-21T22:57:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02523411854Recombinant Protein Expression System in Corynebacterium glutamicum and Its ApplicationMin Ju LeePil KimCorynebacterium glutamicum, a soil-derived gram-positive actinobacterium, has been widely used for the production of biochemical molecules such as amino acids (i.e., L-glutamate and L-lysine), nucleic acids, alcohols, and organic acids. The metabolism of the bacterium has been engineered to increase the production of the target biochemical molecule, which requires a cytosolic enzyme expression. As recent demand for new proteinaceous biologics (such as antibodies, growth factors, and hormones) increase, C. glutamicum is attracting industrial interest as a recombinant protein expression host for therapeutic protein production due to the advantages such as low protease activity without endotoxin activity. In this review, we have summarized the recent studies on the heterologous expression of the recombinant protein in C. glutamicum for metabolic engineering, expansion of substrate availability, and recombinant protein secretion. We have also outlined the advances in genetic components such as promoters, surface anchoring systems, and secretory signal sequences in C. glutamicum for effective recombinant protein expression.https://www.frontiersin.org/article/10.3389/fmicb.2018.02523/fullCorynebacterium glutamiciumexpression host systemscytosolic expressionsecretory expressionrecombinant proteinsurface displayed expression |
| spellingShingle | Min Ju Lee Pil Kim Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application Frontiers in Microbiology Corynebacterium glutamicium expression host systems cytosolic expression secretory expression recombinant protein surface displayed expression |
| title | Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application |
| title_full | Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application |
| title_fullStr | Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application |
| title_full_unstemmed | Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application |
| title_short | Recombinant Protein Expression System in Corynebacterium glutamicum and Its Application |
| title_sort | recombinant protein expression system in corynebacterium glutamicum and its application |
| topic | Corynebacterium glutamicium expression host systems cytosolic expression secretory expression recombinant protein surface displayed expression |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02523/full |
| work_keys_str_mv | AT minjulee recombinantproteinexpressionsystemincorynebacteriumglutamicumanditsapplication AT pilkim recombinantproteinexpressionsystemincorynebacteriumglutamicumanditsapplication |