The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity
Abstract Background and objective: Peroxidases catalyze protein oxidation and lipid peroxidation. The activity of these enzymes in nerve cells is involved in causing disorders such as Alzheimerchr('39')s and Parkinsonchr('39')s disease. This study investigated the effect...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2016-01-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-813-en.html |
| _version_ | 1818157452217024512 |
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| author | Maryam Mohajerani Afsane Aghaei |
| author_facet | Maryam Mohajerani Afsane Aghaei |
| author_sort | Maryam Mohajerani |
| collection | DOAJ |
| description | Abstract
Background and objective: Peroxidases catalyze protein oxidation and lipid peroxidation. The activity of these enzymes in nerve cells is involved in causing disorders such as Alzheimerchr('39')s and Parkinsonchr('39')s disease. This study investigated the effect of Citrus aurantium, Foeniculum vulgare and Rosmarinus officinalis essential oils on activity of peroxidase enzyme.
Methods: All three medicinal plants were dried at room temperature. Their essential oil was extracted by steam distillation using a Clevenger apparatus. Optimal reaction conditions were determined in the presence of hydrogen peroxide and guaiacol as substrate and hydrogen donor, respectively. Enzyme kinetics of zucchini peroxidase were evaluated by increasing the amount of essential oils in optimal reaction conditions. Enzyme reaction rate for each of the essential oils and the Km and Vmax values were determined.
Results: The results indicated concentration-dependent effect of the extracted essential oils on enzyme kinetics at optimum temperature of 50 °C and optimal pH of 6.5. The essential oil of Citrus aurantium had non-competitive inhibitory effects on the enzyme with Km of 6.25 mM, while the enzyme’s Vmax significantly reduced by increasing the concentration. Foeniculum vulgare showed mixed inhibition effect with Km of 7.14 mmol per 20 μl of the essential oil, but had a decreasing effect on the Vmax in smaller amounts. Finally, Rosmarinus officinalis showed activating effects by reducing the Km to 4-5.88 mM.
Conclusion: The essential oils of Citrus aurantium and Foeniculum vulgare are inhibitors of the peroxidase enzyme and can be further studied as natural herbal medicines. |
| first_indexed | 2024-12-11T15:14:25Z |
| format | Article |
| id | doaj.art-ec7c64fbf09c41f1b0ba56e80692cdba |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-11T15:14:25Z |
| publishDate | 2016-01-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-ec7c64fbf09c41f1b0ba56e80692cdba2022-12-22T01:00:38ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492016-01-011011723The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase ActivityMaryam Mohajerani0Afsane Aghaei1 Department of Molecular and Cell Biology Department of Molecular and Cell Biology Abstract Background and objective: Peroxidases catalyze protein oxidation and lipid peroxidation. The activity of these enzymes in nerve cells is involved in causing disorders such as Alzheimerchr('39')s and Parkinsonchr('39')s disease. This study investigated the effect of Citrus aurantium, Foeniculum vulgare and Rosmarinus officinalis essential oils on activity of peroxidase enzyme. Methods: All three medicinal plants were dried at room temperature. Their essential oil was extracted by steam distillation using a Clevenger apparatus. Optimal reaction conditions were determined in the presence of hydrogen peroxide and guaiacol as substrate and hydrogen donor, respectively. Enzyme kinetics of zucchini peroxidase were evaluated by increasing the amount of essential oils in optimal reaction conditions. Enzyme reaction rate for each of the essential oils and the Km and Vmax values were determined. Results: The results indicated concentration-dependent effect of the extracted essential oils on enzyme kinetics at optimum temperature of 50 °C and optimal pH of 6.5. The essential oil of Citrus aurantium had non-competitive inhibitory effects on the enzyme with Km of 6.25 mM, while the enzyme’s Vmax significantly reduced by increasing the concentration. Foeniculum vulgare showed mixed inhibition effect with Km of 7.14 mmol per 20 μl of the essential oil, but had a decreasing effect on the Vmax in smaller amounts. Finally, Rosmarinus officinalis showed activating effects by reducing the Km to 4-5.88 mM. Conclusion: The essential oils of Citrus aurantium and Foeniculum vulgare are inhibitors of the peroxidase enzyme and can be further studied as natural herbal medicines.http://mlj.goums.ac.ir/article-1-813-en.htmlperoxidaseessential oilneurodegenerative diseasesenzyme inhibitors. |
| spellingShingle | Maryam Mohajerani Afsane Aghaei The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity Medical Laboratory Journal peroxidase essential oil neurodegenerative diseases enzyme inhibitors. |
| title | The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity |
| title_full | The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity |
| title_fullStr | The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity |
| title_full_unstemmed | The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity |
| title_short | The Effect of Citrus Aurantium, Foeniculum Vulgare and Rosmarinus Officinalis Essential Oils on Peroxidase Activity |
| title_sort | effect of citrus aurantium foeniculum vulgare and rosmarinus officinalis essential oils on peroxidase activity |
| topic | peroxidase essential oil neurodegenerative diseases enzyme inhibitors. |
| url | http://mlj.goums.ac.ir/article-1-813-en.html |
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