A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor

A one-step incubation ELISA kit based on monoclonal antibody against dibutyl phthalate (DBP) was developed. After optimizing concentrations of coating antigen, antibody and composition of the assay buffer, an inhibition curve was plotted. The IC50 is 29.6 ng·mL-1, and the detection limit for DBP is...

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Main Authors: Sun Quing, Chen Yanli, Li Fuxue, Jia Minghong, Shi Guoqing
Format: Article
Language:English
Published: De Gruyter 2019-07-01
Series:Open Chemistry
Subjects:
Online Access:https://doi.org/10.1515/chem-2019-0052
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author Sun Quing
Chen Yanli
Li Fuxue
Jia Minghong
Shi Guoqing
author_facet Sun Quing
Chen Yanli
Li Fuxue
Jia Minghong
Shi Guoqing
author_sort Sun Quing
collection DOAJ
description A one-step incubation ELISA kit based on monoclonal antibody against dibutyl phthalate (DBP) was developed. After optimizing concentrations of coating antigen, antibody and composition of the assay buffer, an inhibition curve was plotted. The IC50 is 29.6 ng·mL-1, and the detection limit for DBP is 3.6 ng·mL-1. Compared with other ELISA methods, this ELISA kit had a simpler sample preparation, costed less time for detection and could detect more types of sample. The recoveries of DBP in water, beverage and liquor samples were range from 78% to 110.4%, the range of coefficient of variations is 7.7-15.3%. The cross reactivity was very low (&1%) except that for butyl benzyl phthalate (3.9%) and the di-isobutyl phthalate (12.5%). The detection results in liquor showed good correlation with those from GC-MS. All data above indicated that this kit could be used as the fast and high-throughput screening of DBP in water, beverage and liquor.
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spelling doaj.art-ec7fd8bf0dc8475a8ee70628ab58cc552022-12-21T19:14:10ZengDe GruyterOpen Chemistry2391-54202019-07-0117139240010.1515/chem-2019-0052chem-2019-0052A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquorSun Quing0Chen Yanli1Li Fuxue2Jia Minghong3Shi Guoqing4School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, ChinaBeijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products- Beijing University of Agriculture, Beijing, 102206, ChinaBeijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products- Beijing University of Agriculture, Beijing, 102206, ChinaA one-step incubation ELISA kit based on monoclonal antibody against dibutyl phthalate (DBP) was developed. After optimizing concentrations of coating antigen, antibody and composition of the assay buffer, an inhibition curve was plotted. The IC50 is 29.6 ng·mL-1, and the detection limit for DBP is 3.6 ng·mL-1. Compared with other ELISA methods, this ELISA kit had a simpler sample preparation, costed less time for detection and could detect more types of sample. The recoveries of DBP in water, beverage and liquor samples were range from 78% to 110.4%, the range of coefficient of variations is 7.7-15.3%. The cross reactivity was very low (&1%) except that for butyl benzyl phthalate (3.9%) and the di-isobutyl phthalate (12.5%). The detection results in liquor showed good correlation with those from GC-MS. All data above indicated that this kit could be used as the fast and high-throughput screening of DBP in water, beverage and liquor.https://doi.org/10.1515/chem-2019-0052dibutyl phthalateelisa kitone-step incubationbeverageliquor
spellingShingle Sun Quing
Chen Yanli
Li Fuxue
Jia Minghong
Shi Guoqing
A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
Open Chemistry
dibutyl phthalate
elisa kit
one-step incubation
beverage
liquor
title A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
title_full A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
title_fullStr A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
title_full_unstemmed A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
title_short A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
title_sort one step incubation elisa kit for rapid determination of dibutyl phthalate in water beverage and liquor
topic dibutyl phthalate
elisa kit
one-step incubation
beverage
liquor
url https://doi.org/10.1515/chem-2019-0052
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