A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor
A one-step incubation ELISA kit based on monoclonal antibody against dibutyl phthalate (DBP) was developed. After optimizing concentrations of coating antigen, antibody and composition of the assay buffer, an inhibition curve was plotted. The IC50 is 29.6 ng·mL-1, and the detection limit for DBP is...
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Format: | Article |
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De Gruyter
2019-07-01
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Series: | Open Chemistry |
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Online Access: | https://doi.org/10.1515/chem-2019-0052 |
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author | Sun Quing Chen Yanli Li Fuxue Jia Minghong Shi Guoqing |
author_facet | Sun Quing Chen Yanli Li Fuxue Jia Minghong Shi Guoqing |
author_sort | Sun Quing |
collection | DOAJ |
description | A one-step incubation ELISA kit based on monoclonal antibody against dibutyl phthalate (DBP) was developed. After optimizing concentrations of coating antigen, antibody and composition of the assay buffer, an inhibition curve was plotted. The IC50 is 29.6 ng·mL-1, and the detection limit for DBP is 3.6 ng·mL-1. Compared with other ELISA methods, this ELISA kit had a simpler sample preparation, costed less time for detection and could detect more types of sample. The recoveries of DBP in water, beverage and liquor samples were range from 78% to 110.4%, the range of coefficient of variations is 7.7-15.3%. The cross reactivity was very low (&1%) except that for butyl benzyl phthalate (3.9%) and the di-isobutyl phthalate (12.5%). The detection results in liquor showed good correlation with those from GC-MS. All data above indicated that this kit could be used as the fast and high-throughput screening of DBP in water, beverage and liquor. |
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language | English |
last_indexed | 2024-12-21T05:44:16Z |
publishDate | 2019-07-01 |
publisher | De Gruyter |
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series | Open Chemistry |
spelling | doaj.art-ec7fd8bf0dc8475a8ee70628ab58cc552022-12-21T19:14:10ZengDe GruyterOpen Chemistry2391-54202019-07-0117139240010.1515/chem-2019-0052chem-2019-0052A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquorSun Quing0Chen Yanli1Li Fuxue2Jia Minghong3Shi Guoqing4School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, ChinaSchool of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, ChinaBeijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products- Beijing University of Agriculture, Beijing, 102206, ChinaBeijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products- Beijing University of Agriculture, Beijing, 102206, ChinaA one-step incubation ELISA kit based on monoclonal antibody against dibutyl phthalate (DBP) was developed. After optimizing concentrations of coating antigen, antibody and composition of the assay buffer, an inhibition curve was plotted. The IC50 is 29.6 ng·mL-1, and the detection limit for DBP is 3.6 ng·mL-1. Compared with other ELISA methods, this ELISA kit had a simpler sample preparation, costed less time for detection and could detect more types of sample. The recoveries of DBP in water, beverage and liquor samples were range from 78% to 110.4%, the range of coefficient of variations is 7.7-15.3%. The cross reactivity was very low (&1%) except that for butyl benzyl phthalate (3.9%) and the di-isobutyl phthalate (12.5%). The detection results in liquor showed good correlation with those from GC-MS. All data above indicated that this kit could be used as the fast and high-throughput screening of DBP in water, beverage and liquor.https://doi.org/10.1515/chem-2019-0052dibutyl phthalateelisa kitone-step incubationbeverageliquor |
spellingShingle | Sun Quing Chen Yanli Li Fuxue Jia Minghong Shi Guoqing A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor Open Chemistry dibutyl phthalate elisa kit one-step incubation beverage liquor |
title | A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor |
title_full | A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor |
title_fullStr | A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor |
title_full_unstemmed | A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor |
title_short | A one-step incubation ELISA kit for rapid determination of dibutyl phthalate in water, beverage and liquor |
title_sort | one step incubation elisa kit for rapid determination of dibutyl phthalate in water beverage and liquor |
topic | dibutyl phthalate elisa kit one-step incubation beverage liquor |
url | https://doi.org/10.1515/chem-2019-0052 |
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