Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry
Greenhouse gas emissions are a global problem facing the dairy/beef industry. Novel feed additives consisting of seaweeds and hemp containing bioactive compounds are theorized to reduce enteric methane emissions. In this study we aimed to investigate the metabolic profiles of brown, red and green se...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/5/259 |
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| author | Natalja P. Nørskov Annette Bruhn Andrew Cole Mette Olaf Nielsen |
| author_facet | Natalja P. Nørskov Annette Bruhn Andrew Cole Mette Olaf Nielsen |
| author_sort | Natalja P. Nørskov |
| collection | DOAJ |
| description | Greenhouse gas emissions are a global problem facing the dairy/beef industry. Novel feed additives consisting of seaweeds and hemp containing bioactive compounds are theorized to reduce enteric methane emissions. In this study we aimed to investigate the metabolic profiles of brown, red and green seaweeds and hemp using gas chromatography and liquid chromatography mass spectrometry. We used targeted and untargeted approaches, quantifying known halomethanes and phenolics, as well as identifying potentially novel bioactive compounds with anti-methanogenic properties. The main findings were: (a) <i>Asparagopsis taxiformis</i> contained halomethanes, with high concentrations of bromoform (4200 µg/g DW), six volatile halocarbons were tentatively identified; (b) no halomethanes were detected in the other studied seaweeds nor in hemp; (c) high concentrations of lignans were measured in hemp; (d) a high numbers of sulfated phenolic acids and unidentified sulfuric acid-containing compounds were detected in all seaweeds; (e) flavonoid glucosides and glucuronides were mainly identified in hemp; and (f) the condensed tannin gallocatechin was tentatively identified in <i>Fucus</i> sp. Using the combined metabolomics approach, an overview and in-depth information on secondary metabolites were provided. Halomethanes of <i>Asparagopsis</i> sp. have already been shown to be anti-methanogenic; however, metabolic profiles of seaweeds such as <i>Dictyota</i> and <i>Sargassum</i> have also been shown to contain compounds that may have anti-methanogenic potential. |
| first_indexed | 2024-03-10T12:05:13Z |
| format | Article |
| id | doaj.art-ec82e1dd56014df7855ad45e62926642 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T12:05:13Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-ec82e1dd56014df7855ad45e629266422023-11-21T16:41:55ZengMDPI AGMetabolites2218-19892021-04-0111525910.3390/metabo11050259Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass SpectrometryNatalja P. Nørskov0Annette Bruhn1Andrew Cole2Mette Olaf Nielsen3Department of Animal Science, Aarhus University, Blichers Alle 20, 8830 Tjele, DenmarkCenter for Circular Bioeconomy, Aarhus University, 8830 Tjele, DenmarkCenter for Macroalgal Resources and Biotechnology, James Cook University, Douglas, QLD 4811, AustraliaDepartment of Animal Science, Aarhus University, Blichers Alle 20, 8830 Tjele, DenmarkGreenhouse gas emissions are a global problem facing the dairy/beef industry. Novel feed additives consisting of seaweeds and hemp containing bioactive compounds are theorized to reduce enteric methane emissions. In this study we aimed to investigate the metabolic profiles of brown, red and green seaweeds and hemp using gas chromatography and liquid chromatography mass spectrometry. We used targeted and untargeted approaches, quantifying known halomethanes and phenolics, as well as identifying potentially novel bioactive compounds with anti-methanogenic properties. The main findings were: (a) <i>Asparagopsis taxiformis</i> contained halomethanes, with high concentrations of bromoform (4200 µg/g DW), six volatile halocarbons were tentatively identified; (b) no halomethanes were detected in the other studied seaweeds nor in hemp; (c) high concentrations of lignans were measured in hemp; (d) a high numbers of sulfated phenolic acids and unidentified sulfuric acid-containing compounds were detected in all seaweeds; (e) flavonoid glucosides and glucuronides were mainly identified in hemp; and (f) the condensed tannin gallocatechin was tentatively identified in <i>Fucus</i> sp. Using the combined metabolomics approach, an overview and in-depth information on secondary metabolites were provided. Halomethanes of <i>Asparagopsis</i> sp. have already been shown to be anti-methanogenic; however, metabolic profiles of seaweeds such as <i>Dictyota</i> and <i>Sargassum</i> have also been shown to contain compounds that may have anti-methanogenic potential.https://www.mdpi.com/2218-1989/11/5/259metabolomic profilingseaweedshempphenolicshalomethanesmethane |
| spellingShingle | Natalja P. Nørskov Annette Bruhn Andrew Cole Mette Olaf Nielsen Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry Metabolites metabolomic profiling seaweeds hemp phenolics halomethanes methane |
| title | Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry |
| title_full | Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry |
| title_fullStr | Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry |
| title_full_unstemmed | Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry |
| title_short | Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry |
| title_sort | targeted and untargeted metabolic profiling to discover bioactive compounds in seaweeds and hemp using gas and liquid chromatography mass spectrometry |
| topic | metabolomic profiling seaweeds hemp phenolics halomethanes methane |
| url | https://www.mdpi.com/2218-1989/11/5/259 |
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