The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication
Cilia are hair-like projections of the plasma membrane with an inner microtubule skeleton known as axoneme. Motile cilia and flagella beat to displace extracellular fluids, playing important roles in the airways and reproductive system. On the contrary, primary cilia function as cell-type-dependent...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2073-4409/12/1/142 |
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| author | Pablo López-Jiménez Sara Pérez-Martín Inés Hidalgo Francesc R. García-Gonzalo Jesús Page Rocio Gómez |
| author_facet | Pablo López-Jiménez Sara Pérez-Martín Inés Hidalgo Francesc R. García-Gonzalo Jesús Page Rocio Gómez |
| author_sort | Pablo López-Jiménez |
| collection | DOAJ |
| description | Cilia are hair-like projections of the plasma membrane with an inner microtubule skeleton known as axoneme. Motile cilia and flagella beat to displace extracellular fluids, playing important roles in the airways and reproductive system. On the contrary, primary cilia function as cell-type-dependent sensory organelles, detecting chemical, mechanical, or optical signals from the extracellular environment. Cilia dysfunction is associated with genetic diseases called ciliopathies and with some types of cancer. Cilia have been recently identified in zebrafish gametogenesis as an important regulator of <i>bouquet</i> conformation and recombination. However, there is little information about the structure and functions of cilia in mammalian meiosis. Here we describe the presence of cilia in male mouse meiotic cells. These solitary cilia formed transiently in 20% of zygotene spermatocytes and reached considerable lengths (up to 15–23 µm). CEP164 and CETN3 localization studies indicated that these cilia emanate from the mother centriole prior to centrosome duplication. In addition, the study of telomeric TFR2 suggested that cilia are not directly related to the <i>bouquet</i> conformation during early male mouse meiosis. Instead, based on TEX14 labeling of intercellular bridges in spermatocyte cysts, we suggest that mouse meiotic cilia may have sensory roles affecting cyst function during prophase I. |
| first_indexed | 2024-03-11T10:05:23Z |
| format | Article |
| id | doaj.art-ec8392fe083b4ee7b0e5d33d141c2eff |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T10:05:23Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-ec8392fe083b4ee7b0e5d33d141c2eff2023-11-16T15:06:40ZengMDPI AGCells2073-44092022-12-0112114210.3390/cells12010142The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome DuplicationPablo López-Jiménez0Sara Pérez-Martín1Inés Hidalgo2Francesc R. García-Gonzalo3Jesús Page4Rocio Gómez5Unidad de Biología Celular, Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, SpainUnidad de Biología Celular, Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, SpainUnidad de Biología Celular, Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, SpainDepartamento de Bioquímica, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, SpainUnidad de Biología Celular, Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, SpainUnidad de Biología Celular, Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, SpainCilia are hair-like projections of the plasma membrane with an inner microtubule skeleton known as axoneme. Motile cilia and flagella beat to displace extracellular fluids, playing important roles in the airways and reproductive system. On the contrary, primary cilia function as cell-type-dependent sensory organelles, detecting chemical, mechanical, or optical signals from the extracellular environment. Cilia dysfunction is associated with genetic diseases called ciliopathies and with some types of cancer. Cilia have been recently identified in zebrafish gametogenesis as an important regulator of <i>bouquet</i> conformation and recombination. However, there is little information about the structure and functions of cilia in mammalian meiosis. Here we describe the presence of cilia in male mouse meiotic cells. These solitary cilia formed transiently in 20% of zygotene spermatocytes and reached considerable lengths (up to 15–23 µm). CEP164 and CETN3 localization studies indicated that these cilia emanate from the mother centriole prior to centrosome duplication. In addition, the study of telomeric TFR2 suggested that cilia are not directly related to the <i>bouquet</i> conformation during early male mouse meiosis. Instead, based on TEX14 labeling of intercellular bridges in spermatocyte cysts, we suggest that mouse meiotic cilia may have sensory roles affecting cyst function during prophase I.https://www.mdpi.com/2073-4409/12/1/142meiosisciliacentrosomemouse |
| spellingShingle | Pablo López-Jiménez Sara Pérez-Martín Inés Hidalgo Francesc R. García-Gonzalo Jesús Page Rocio Gómez The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication Cells meiosis cilia centrosome mouse |
| title | The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication |
| title_full | The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication |
| title_fullStr | The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication |
| title_full_unstemmed | The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication |
| title_short | The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication |
| title_sort | male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication |
| topic | meiosis cilia centrosome mouse |
| url | https://www.mdpi.com/2073-4409/12/1/142 |
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