Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesions
Abstract Background Oral potentially malignant lesions (OPML) are suspicious lesions that may turn into malignancy according to the degree of dysplasia. Early diagnosis of these lesions allows their treatment before malignant transformation. Saliva is a liquid biopsy that can be obtained easily from...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-09-01
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| Series: | Bulletin of the National Research Centre |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42269-022-00931-9 |
| _version_ | 1798004403155763200 |
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| author | Heba E. Tarek Heba N. Shalash Reham A. A. Morsy Basma Mostafa Mirhane Hassan Rania M. Abbas Marwa M. Ellithy |
| author_facet | Heba E. Tarek Heba N. Shalash Reham A. A. Morsy Basma Mostafa Mirhane Hassan Rania M. Abbas Marwa M. Ellithy |
| author_sort | Heba E. Tarek |
| collection | DOAJ |
| description | Abstract Background Oral potentially malignant lesions (OPML) are suspicious lesions that may turn into malignancy according to the degree of dysplasia. Early diagnosis of these lesions allows their treatment before malignant transformation. Saliva is a liquid biopsy that can be obtained easily from the patient and assessed for biomarkers expression. Our study aimed to investigate the possibility of using salivary and serum TNF-α and Ki-67 in early detection of OPML. Patients with OPML were selected according to histopathological evaluation of the suspected lesions. The lesions were stained immunohistochemically by tumor necrosis factor alpha (TNF-α) and Ki-67. Saliva and blood samples were collected from the patients for detecting the expression of the markers by ELISA. Results The level of TNF-α and Ki-67 was higher in patients with OPML than healthy individuals regarding both saliva and serum. TNF-α was significantly higher in saliva than in serum unlike Ki-67 which was insignificantly higher in saliva than in serum. The OPML show negative expression of TNF-α in OPML immunohistochemically unlike Ki-67 which show intense positive expression in OPML immunohistochemically. Conclusions Saliva can be used as an early noninvasive biomarker detector depending on selecting the appropriate salivary biomarker. Salivary TNF-α can be used in early diagnosis of OPML. |
| first_indexed | 2024-04-11T12:23:06Z |
| format | Article |
| id | doaj.art-ec8469b102e3411bb19a02b5e4d327bd |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-04-11T12:23:06Z |
| publishDate | 2022-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-ec8469b102e3411bb19a02b5e4d327bd2022-12-22T04:24:02ZengSpringerOpenBulletin of the National Research Centre2522-83072022-09-0146111410.1186/s42269-022-00931-9Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesionsHeba E. Tarek0Heba N. Shalash1Reham A. A. Morsy2Basma Mostafa3Mirhane Hassan4Rania M. Abbas5Marwa M. Ellithy6Basic Dental Science Department, Oral and Dental Research Institute, National Research CentreBasic Dental Science Department, Oral and Dental Research Institute, National Research CentreBasic Dental Science Department, Oral and Dental Research Institute, National Research CentreSurgery and Oral Medicine Department, Oral and Dental Research Institute, National Research CentreClinical and Chemical Pathology Department, Medical Research Institute, National Research CentreClinical Pathology Department, Ain Shams UniversityBasic Dental Science Department, Oral and Dental Research Institute, National Research CentreAbstract Background Oral potentially malignant lesions (OPML) are suspicious lesions that may turn into malignancy according to the degree of dysplasia. Early diagnosis of these lesions allows their treatment before malignant transformation. Saliva is a liquid biopsy that can be obtained easily from the patient and assessed for biomarkers expression. Our study aimed to investigate the possibility of using salivary and serum TNF-α and Ki-67 in early detection of OPML. Patients with OPML were selected according to histopathological evaluation of the suspected lesions. The lesions were stained immunohistochemically by tumor necrosis factor alpha (TNF-α) and Ki-67. Saliva and blood samples were collected from the patients for detecting the expression of the markers by ELISA. Results The level of TNF-α and Ki-67 was higher in patients with OPML than healthy individuals regarding both saliva and serum. TNF-α was significantly higher in saliva than in serum unlike Ki-67 which was insignificantly higher in saliva than in serum. The OPML show negative expression of TNF-α in OPML immunohistochemically unlike Ki-67 which show intense positive expression in OPML immunohistochemically. Conclusions Saliva can be used as an early noninvasive biomarker detector depending on selecting the appropriate salivary biomarker. Salivary TNF-α can be used in early diagnosis of OPML.https://doi.org/10.1186/s42269-022-00931-9Oral potentially malignant lesionsSalivary biomarkersSerum biomarkersTNF-αKi-67 |
| spellingShingle | Heba E. Tarek Heba N. Shalash Reham A. A. Morsy Basma Mostafa Mirhane Hassan Rania M. Abbas Marwa M. Ellithy Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesions Bulletin of the National Research Centre Oral potentially malignant lesions Salivary biomarkers Serum biomarkers TNF-α Ki-67 |
| title | Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesions |
| title_full | Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesions |
| title_fullStr | Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesions |
| title_full_unstemmed | Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesions |
| title_short | Salivary and serum expression of TNF-α and Ki-67 in oral potentially malignant lesions |
| title_sort | salivary and serum expression of tnf α and ki 67 in oral potentially malignant lesions |
| topic | Oral potentially malignant lesions Salivary biomarkers Serum biomarkers TNF-α Ki-67 |
| url | https://doi.org/10.1186/s42269-022-00931-9 |
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