Mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is a primary malignancy with increasing incidence and poor prognosis. Heterogeneity originating from genomic instability is one of the critical reasons of poor outcomes. However, the studies of underlying mechanisms and pathways affected by mutations are still not inte...

Olles dieđut

Bibliográfalaš dieđut
Váldodahkkit: Qiong Wu, Lingyi Wang, Stephen Kwok-Wing Tsui
Materiálatiipa: Artihkal
Giella:English
Almmustuhtton: Frontiers Media S.A. 2022-08-01
Ráidu:Frontiers in Genetics
Fáttát:
Liŋkkat:https://www.frontiersin.org/articles/10.3389/fgene.2022.970907/full
_version_ 1828758608065593344
author Qiong Wu
Qiong Wu
Lingyi Wang
Stephen Kwok-Wing Tsui
author_facet Qiong Wu
Qiong Wu
Lingyi Wang
Stephen Kwok-Wing Tsui
author_sort Qiong Wu
collection DOAJ
description Hepatocellular carcinoma (HCC) is a primary malignancy with increasing incidence and poor prognosis. Heterogeneity originating from genomic instability is one of the critical reasons of poor outcomes. However, the studies of underlying mechanisms and pathways affected by mutations are still not intelligible. Currently, integrative molecular-level studies using multiomics approaches enable comprehensive analysis for cancers, which is pivotal for personalized therapy and mortality reduction. In this study, genomic and transcriptomic data of HCC are obtained from The Cancer Genome Atlas (TCGA) to investigate the affected coding and non-coding RNAs, as well as their regulatory network due to certain mutational signatures of HCC. Different types of RNAs have their specific enriched biological functions in mutational signature-specific HCCs, upregulated coding RNAs are predominantly associated with lipid metabolism-related pathways, and downregulated coding RNAs are enriched in axonogenesis for tumor microenvironment generation. Additionally, differentially expressed miRNAs are inclined to concentrate in cancer-related signaling pathways. Some of these RNAs also serve as prognostic factors that help predict the survival outcome of HCCs with certain mutational signatures. Furthermore, deregulation of competing endogenous RNA (ceRNA) regulatory network is identified, which suggests a potential therapy via interference of miRNA activity for mutational signature-specific HCC. This study proposes a projection approach to reduce therapeutic complexity from genomic mutations to transcriptomic alterations. Through this method, we identify genes and pathways critical for mutational signature-specific HCC and further discover a series of prognostic markers indicating patient survival outcome.
first_indexed 2024-12-11T00:44:34Z
format Article
id doaj.art-ec84c1707e8e4dbab736de826bac1905
institution Directory Open Access Journal
issn 1664-8021
language English
last_indexed 2024-12-11T00:44:34Z
publishDate 2022-08-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Genetics
spelling doaj.art-ec84c1707e8e4dbab736de826bac19052022-12-22T01:26:49ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-08-011310.3389/fgene.2022.970907970907Mutational signatures representative transcriptomic perturbations in hepatocellular carcinomaQiong Wu0Qiong Wu1Lingyi Wang2Stephen Kwok-Wing Tsui3School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaDepartment of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaSchool of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, ChinaHepatocellular carcinoma (HCC) is a primary malignancy with increasing incidence and poor prognosis. Heterogeneity originating from genomic instability is one of the critical reasons of poor outcomes. However, the studies of underlying mechanisms and pathways affected by mutations are still not intelligible. Currently, integrative molecular-level studies using multiomics approaches enable comprehensive analysis for cancers, which is pivotal for personalized therapy and mortality reduction. In this study, genomic and transcriptomic data of HCC are obtained from The Cancer Genome Atlas (TCGA) to investigate the affected coding and non-coding RNAs, as well as their regulatory network due to certain mutational signatures of HCC. Different types of RNAs have their specific enriched biological functions in mutational signature-specific HCCs, upregulated coding RNAs are predominantly associated with lipid metabolism-related pathways, and downregulated coding RNAs are enriched in axonogenesis for tumor microenvironment generation. Additionally, differentially expressed miRNAs are inclined to concentrate in cancer-related signaling pathways. Some of these RNAs also serve as prognostic factors that help predict the survival outcome of HCCs with certain mutational signatures. Furthermore, deregulation of competing endogenous RNA (ceRNA) regulatory network is identified, which suggests a potential therapy via interference of miRNA activity for mutational signature-specific HCC. This study proposes a projection approach to reduce therapeutic complexity from genomic mutations to transcriptomic alterations. Through this method, we identify genes and pathways critical for mutational signature-specific HCC and further discover a series of prognostic markers indicating patient survival outcome.https://www.frontiersin.org/articles/10.3389/fgene.2022.970907/fullmultiomicsmutational signaturemiRNAlncRNAceRNA network
spellingShingle Qiong Wu
Qiong Wu
Lingyi Wang
Stephen Kwok-Wing Tsui
Mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma
Frontiers in Genetics
multiomics
mutational signature
miRNA
lncRNA
ceRNA network
title Mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma
title_full Mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma
title_fullStr Mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma
title_full_unstemmed Mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma
title_short Mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma
title_sort mutational signatures representative transcriptomic perturbations in hepatocellular carcinoma
topic multiomics
mutational signature
miRNA
lncRNA
ceRNA network
url https://www.frontiersin.org/articles/10.3389/fgene.2022.970907/full
work_keys_str_mv AT qiongwu mutationalsignaturesrepresentativetranscriptomicperturbationsinhepatocellularcarcinoma
AT qiongwu mutationalsignaturesrepresentativetranscriptomicperturbationsinhepatocellularcarcinoma
AT lingyiwang mutationalsignaturesrepresentativetranscriptomicperturbationsinhepatocellularcarcinoma
AT stephenkwokwingtsui mutationalsignaturesrepresentativetranscriptomicperturbationsinhepatocellularcarcinoma