Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis
The exact mechanisms of nucleic acid (NA) delivery with gene electrotransfer (GET) are still unknown, which represents a limitation for its broader use. Further, not knowing the effects that different experimental electrical and biological parameters have on GET additionally hinders GET optimization...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/12/2700 |
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| author | Tjaša Potočnik Alenka Maček Lebar Špela Kos Matej Reberšek Eva Pirc Gregor Serša Damijan Miklavčič |
| author_facet | Tjaša Potočnik Alenka Maček Lebar Špela Kos Matej Reberšek Eva Pirc Gregor Serša Damijan Miklavčič |
| author_sort | Tjaša Potočnik |
| collection | DOAJ |
| description | The exact mechanisms of nucleic acid (NA) delivery with gene electrotransfer (GET) are still unknown, which represents a limitation for its broader use. Further, not knowing the effects that different experimental electrical and biological parameters have on GET additionally hinders GET optimization, resulting in the majority of research being performed using a trial-and-error approach. To explore the current state of knowledge, we conducted a systematic literature review of GET papers in in vitro conditions and performed meta-analyses of the reported GET efficiency. For now, there is no universal GET strategy that would be appropriate for all experimental aims. Apart from the availability of the required electroporation device and electrodes, the choice of an optimal GET approach depends on parameters such as the electroporation medium; type and origin of cells; and the size, concentration, promoter, and type of the NA to be transfected. Equally important are appropriate controls and the measurement or evaluation of the output pulses to allow a fair and unbiased evaluation of the experimental results. Since many experimental electrical and biological parameters can affect GET, it is important that all used parameters are adequately reported to enable the comparison of results, as well as potentially faster and more efficient experiment planning and optimization. |
| first_indexed | 2024-03-09T15:58:52Z |
| format | Article |
| id | doaj.art-ec855659dc454da794fd899e15e0bcdb |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:58:52Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-ec855659dc454da794fd899e15e0bcdb2023-11-24T17:20:34ZengMDPI AGPharmaceutics1999-49232022-12-011412270010.3390/pharmaceutics14122700Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-AnalysisTjaša Potočnik0Alenka Maček Lebar1Špela Kos2Matej Reberšek3Eva Pirc4Gregor Serša5Damijan Miklavčič6Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, SloveniaFaculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, SloveniaDepartment of Experimental Oncology, Institute of Oncology Ljubljana, Zaloška cesta 2, 1000 Ljubljana, SloveniaFaculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, SloveniaFaculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, SloveniaDepartment of Experimental Oncology, Institute of Oncology Ljubljana, Zaloška cesta 2, 1000 Ljubljana, SloveniaFaculty of Electrical Engineering, University of Ljubljana, Tržaška 25, 1000 Ljubljana, SloveniaThe exact mechanisms of nucleic acid (NA) delivery with gene electrotransfer (GET) are still unknown, which represents a limitation for its broader use. Further, not knowing the effects that different experimental electrical and biological parameters have on GET additionally hinders GET optimization, resulting in the majority of research being performed using a trial-and-error approach. To explore the current state of knowledge, we conducted a systematic literature review of GET papers in in vitro conditions and performed meta-analyses of the reported GET efficiency. For now, there is no universal GET strategy that would be appropriate for all experimental aims. Apart from the availability of the required electroporation device and electrodes, the choice of an optimal GET approach depends on parameters such as the electroporation medium; type and origin of cells; and the size, concentration, promoter, and type of the NA to be transfected. Equally important are appropriate controls and the measurement or evaluation of the output pulses to allow a fair and unbiased evaluation of the experimental results. Since many experimental electrical and biological parameters can affect GET, it is important that all used parameters are adequately reported to enable the comparison of results, as well as potentially faster and more efficient experiment planning and optimization.https://www.mdpi.com/1999-4923/14/12/2700gene electrotransferplasmid DNAnucleic acidpulse parameterselectroporation mediumcell line |
| spellingShingle | Tjaša Potočnik Alenka Maček Lebar Špela Kos Matej Reberšek Eva Pirc Gregor Serša Damijan Miklavčič Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis Pharmaceutics gene electrotransfer plasmid DNA nucleic acid pulse parameters electroporation medium cell line |
| title | Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis |
| title_full | Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis |
| title_fullStr | Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis |
| title_full_unstemmed | Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis |
| title_short | Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis |
| title_sort | effect of experimental electrical and biological parameters on gene transfer by electroporation a systematic review and meta analysis |
| topic | gene electrotransfer plasmid DNA nucleic acid pulse parameters electroporation medium cell line |
| url | https://www.mdpi.com/1999-4923/14/12/2700 |
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