Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation
A simple and efficient reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the first time for the estimation of a mosquito repellent, methyl jasmonate in a cream formulation, and validated as per the International Conference on Harmonization guidelines. Ace...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-05-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019322066 |
| _version_ | 1818512427131600896 |
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| author | Johirul Islam Saurav Phukan Pronobesh Chattopadhyay |
| author_facet | Johirul Islam Saurav Phukan Pronobesh Chattopadhyay |
| author_sort | Johirul Islam |
| collection | DOAJ |
| description | A simple and efficient reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the first time for the estimation of a mosquito repellent, methyl jasmonate in a cream formulation, and validated as per the International Conference on Harmonization guidelines. Acetonitrile and water (75:25 v/v) were used as the mobile phase and the flow-rate of the mobile phase was kept constant at 1.0 mL/min. The analysis was performed isocratically on a C18 analytical column (250 × 4.4 mm, 5 μm) using a Diode Array Detector for the detection of methyl jasmonate at 214 nm. The presence of excipients did not interfere with the quantification of methyl jasmonate. The calibration curve was linear in the concentration range of 25–300 μg/mL. The relative standard deviations for intra-day and inter-day precision, and repeatability were less than 2%. The recovery ranged from 88.5% to 90.7% with relative standard deviations not higher than 2%. The limit of detection and quantification were 9.4 μg/mL and 28.5 μg/mL, respectively. System suitability parameters were within the accepted range. The proposed method was also robust. Thus, the present report puts forward a novel analytical method for the estimation of an emerging mosquito repellent, methyl jasmonate by using the RP-HPLC technique. |
| first_indexed | 2024-12-10T23:46:44Z |
| format | Article |
| id | doaj.art-ec881344bd044744a03de2b93cb13e29 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T23:46:44Z |
| publishDate | 2019-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-ec881344bd044744a03de2b93cb13e292022-12-22T01:28:54ZengElsevierHeliyon2405-84402019-05-0155e01775Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulationJohirul Islam0Saurav Phukan1Pronobesh Chattopadhyay2Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 784001, Assam, India; Corresponding author.Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784 028, Assam, IndiaDivision of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 784001, Assam, IndiaA simple and efficient reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the first time for the estimation of a mosquito repellent, methyl jasmonate in a cream formulation, and validated as per the International Conference on Harmonization guidelines. Acetonitrile and water (75:25 v/v) were used as the mobile phase and the flow-rate of the mobile phase was kept constant at 1.0 mL/min. The analysis was performed isocratically on a C18 analytical column (250 × 4.4 mm, 5 μm) using a Diode Array Detector for the detection of methyl jasmonate at 214 nm. The presence of excipients did not interfere with the quantification of methyl jasmonate. The calibration curve was linear in the concentration range of 25–300 μg/mL. The relative standard deviations for intra-day and inter-day precision, and repeatability were less than 2%. The recovery ranged from 88.5% to 90.7% with relative standard deviations not higher than 2%. The limit of detection and quantification were 9.4 μg/mL and 28.5 μg/mL, respectively. System suitability parameters were within the accepted range. The proposed method was also robust. Thus, the present report puts forward a novel analytical method for the estimation of an emerging mosquito repellent, methyl jasmonate by using the RP-HPLC technique.http://www.sciencedirect.com/science/article/pii/S2405844019322066Pharmaceutical chemistryAnalytical chemistry |
| spellingShingle | Johirul Islam Saurav Phukan Pronobesh Chattopadhyay Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation Heliyon Pharmaceutical chemistry Analytical chemistry |
| title | Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation |
| title_full | Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation |
| title_fullStr | Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation |
| title_full_unstemmed | Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation |
| title_short | Development of a validated RP-HPLC/DAD method for the quantitative determination of methyl jasmonate in an insect repellent semi-solid formulation |
| title_sort | development of a validated rp hplc dad method for the quantitative determination of methyl jasmonate in an insect repellent semi solid formulation |
| topic | Pharmaceutical chemistry Analytical chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019322066 |
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