Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell Detachment
<i>Trichomonas vaginalis</i>, a protozoan parasite specific to the human genital tract, is one of the most common sexually transmitted pathogens. Its pathogenicity is strongly associated with its expression of a broad array of proteases triggering cytotoxic effects in host epithelial cel...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/15/11/1350 |
| _version_ | 1797466936675663872 |
|---|---|
| author | Bénédicte Pradines Séverine Domenichini Vanessa Lievin-Le Moal |
| author_facet | Bénédicte Pradines Séverine Domenichini Vanessa Lievin-Le Moal |
| author_sort | Bénédicte Pradines |
| collection | DOAJ |
| description | <i>Trichomonas vaginalis</i>, a protozoan parasite specific to the human genital tract, is one of the most common sexually transmitted pathogens. Its pathogenicity is strongly associated with its expression of a broad array of proteases triggering cytotoxic effects in host epithelial cells. Vaginal microbiota-associated <i>Lactobacillus</i>, including those of <i>L. gasseri</i> in particular, can counteract <i>T. vaginalis</i> pathogenesis, but the mechanisms involved have yet to be clarified. <i>T. vaginalis</i> strain G3 (<i>Tv</i> G3) cytotoxicity was assessed by examining cell morphology, cell detachment, and fluorescent labeling of the F-actin cytoskeleton and immunolabeling of vinculin-position focal adhesions (FAs) by confocal laser scanning electron microscopy on confluent cervicovaginal epithelial HeLa cell monolayers. The inhibitory effects of bacterial cells and secreted products of <i>L. gasseri</i> ATCC 9857 and KS 120.1 on the <i>Tv</i> G3 viability and parasite deleterious effects on HeLa cells were investigated. Pre-adhering <i>L. gasseri</i> cells delayed but did not inhibit <i>Tv</i> G3-induced cell detachment, F-actin cytoskeleton disorganization and the disappearance of vinculin-positive focal FAs. <i>L. gasseri</i> KS 120.1 secretion products had a rapid parasiticide activity by killing time- and concentration-dependent <i>Tv</i> G3 parasites after direct contact. By killing <i>Tv</i> G3 parasites already associated with the epithelial cells, secretion products have abolished parasite-induced cell detachment. Our findings suggest that vagina microbiota-associated <i>L. gasseri</i> creates a physical barrier and exerts pharmacological-type mechanisms to counteract the deleterious cytotoxic effects of <i>T. vaginalis.</i> |
| first_indexed | 2024-03-09T18:45:33Z |
| format | Article |
| id | doaj.art-ec8ebfd76c1c4e4393826b51053a8b2c |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T18:45:33Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-ec8ebfd76c1c4e4393826b51053a8b2c2023-11-24T06:18:32ZengMDPI AGPharmaceuticals1424-82472022-10-011511135010.3390/ph15111350Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell DetachmentBénédicte Pradines0Séverine Domenichini1Vanessa Lievin-Le Moal2Inserm, UMR-S 996 Inflammation, Microbiome and Immunosurveillance, University Paris-Saclay, 91400 Orsay, FranceUnité Mixte de Services, Ingénierie et Plateformes au Service de l’Innovation Thérapeutique (UMS-IPSIT), Microscopy Facility, Université Paris-Saclay, 91400 Orsay, FranceInserm, UMR-S 996 Inflammation, Microbiome and Immunosurveillance, University Paris-Saclay, 91400 Orsay, France<i>Trichomonas vaginalis</i>, a protozoan parasite specific to the human genital tract, is one of the most common sexually transmitted pathogens. Its pathogenicity is strongly associated with its expression of a broad array of proteases triggering cytotoxic effects in host epithelial cells. Vaginal microbiota-associated <i>Lactobacillus</i>, including those of <i>L. gasseri</i> in particular, can counteract <i>T. vaginalis</i> pathogenesis, but the mechanisms involved have yet to be clarified. <i>T. vaginalis</i> strain G3 (<i>Tv</i> G3) cytotoxicity was assessed by examining cell morphology, cell detachment, and fluorescent labeling of the F-actin cytoskeleton and immunolabeling of vinculin-position focal adhesions (FAs) by confocal laser scanning electron microscopy on confluent cervicovaginal epithelial HeLa cell monolayers. The inhibitory effects of bacterial cells and secreted products of <i>L. gasseri</i> ATCC 9857 and KS 120.1 on the <i>Tv</i> G3 viability and parasite deleterious effects on HeLa cells were investigated. Pre-adhering <i>L. gasseri</i> cells delayed but did not inhibit <i>Tv</i> G3-induced cell detachment, F-actin cytoskeleton disorganization and the disappearance of vinculin-positive focal FAs. <i>L. gasseri</i> KS 120.1 secretion products had a rapid parasiticide activity by killing time- and concentration-dependent <i>Tv</i> G3 parasites after direct contact. By killing <i>Tv</i> G3 parasites already associated with the epithelial cells, secretion products have abolished parasite-induced cell detachment. Our findings suggest that vagina microbiota-associated <i>L. gasseri</i> creates a physical barrier and exerts pharmacological-type mechanisms to counteract the deleterious cytotoxic effects of <i>T. vaginalis.</i>https://www.mdpi.com/1424-8247/15/11/1350<i>Trichomonas vaginalis</i>motilityadhesioncytotoxicityvaginal microbiota<i>Lactobacillus gasseri</i> |
| spellingShingle | Bénédicte Pradines Séverine Domenichini Vanessa Lievin-Le Moal Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell Detachment Pharmaceuticals <i>Trichomonas vaginalis</i> motility adhesion cytotoxicity vaginal microbiota <i>Lactobacillus gasseri</i> |
| title | Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell Detachment |
| title_full | Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell Detachment |
| title_fullStr | Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell Detachment |
| title_full_unstemmed | Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell Detachment |
| title_short | Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated <i>Lactobacillus gasseri</i> Confer Non-Identical Cell Protection against <i>Trichomonas vaginalis</i>-Induced Cell Detachment |
| title_sort | adherent bacteria and parasiticidal secretion products of human cervicovaginal microbiota associated i lactobacillus gasseri i confer non identical cell protection against i trichomonas vaginalis i induced cell detachment |
| topic | <i>Trichomonas vaginalis</i> motility adhesion cytotoxicity vaginal microbiota <i>Lactobacillus gasseri</i> |
| url | https://www.mdpi.com/1424-8247/15/11/1350 |
| work_keys_str_mv | AT benedictepradines adherentbacteriaandparasiticidalsecretionproductsofhumancervicovaginalmicrobiotaassociatedilactobacillusgasseriiconfernonidenticalcellprotectionagainstitrichomonasvaginalisiinducedcelldetachment AT severinedomenichini adherentbacteriaandparasiticidalsecretionproductsofhumancervicovaginalmicrobiotaassociatedilactobacillusgasseriiconfernonidenticalcellprotectionagainstitrichomonasvaginalisiinducedcelldetachment AT vanessalievinlemoal adherentbacteriaandparasiticidalsecretionproductsofhumancervicovaginalmicrobiotaassociatedilactobacillusgasseriiconfernonidenticalcellprotectionagainstitrichomonasvaginalisiinducedcelldetachment |