Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions
Relevance. Currently, the participation of immune system cells in the regulation of reparative processes is attracting more and more attention of researchers. There is an anatomical connection between the liver and spleen by means of portal vein. Thus, cytokines and other biologically active substan...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Peoples’ Friendship University of Russia (RUDN University)
2023-12-01
|
| Series: | RUDN Journal of Medicine |
| Subjects: | |
| Online Access: | https://journals.rudn.ru/medicine/article/viewFile/37170/22870 |
| _version_ | 1797375549840031744 |
|---|---|
| author | Aiaz T. Mamedov Elena A. Gantsova Viktoria V. Kiseleva Anastasia V. Lokhonina Andrey V. Makarov Svetlana A. Turygina Irina A. Bicherova Irina V. Arutyunyan Polina A. Vishnyakova Andrey V. Elchaninov Timur Kh. Fatkhudinov |
| author_facet | Aiaz T. Mamedov Elena A. Gantsova Viktoria V. Kiseleva Anastasia V. Lokhonina Andrey V. Makarov Svetlana A. Turygina Irina A. Bicherova Irina V. Arutyunyan Polina A. Vishnyakova Andrey V. Elchaninov Timur Kh. Fatkhudinov |
| author_sort | Aiaz T. Mamedov |
| collection | DOAJ |
| description | Relevance. Currently, the participation of immune system cells in the regulation of reparative processes is attracting more and more attention of researchers. There is an anatomical connection between the liver and spleen by means of portal vein. Thus, cytokines and other biologically active substances can enter the liver from the spleen through the portal vein, as well as cells can migrate to the liver. However, the specific mechanisms of mutual influence of the mentioned organs, including in reparative processes, remain poorly studied. The aim of our work was to study the state of spleen monocyte-macrophage population after liver resection, as well as the proliferative activity of spleen cells during liver regeneration . Materials and Methods . The model of liver regeneration after 70 % resection in mouse was reproduced in this work. The animals were taken out of the experiment after 1, 3 and 7 days. The marker of cell proliferation Ki67 was immunohistochemically detected, the state of spleen monocyte-macrophage population was evaluated by markers CD68, CD115, CD206, F4/80 by methods of immunohistochemistry and flow cytometry. Results and Discussion . The liver regeneration had a pronounced effect on the cytoarchitectonics of the spleen. In 1 day after liver resection in the spleen there was observed a decrease in the share of Ki67+cells, according to the flow cytometry data there was a decrease in the number of CD115+cells, in 3 and 7 days there was a decrease in the number of F4/80+ macrophages. Conclusion . Liver resection causes changes in the state of cell populations of the spleen as well. First of all, to the decrease in the activity of proliferative processes in it, as well as to the changes in the state of the monocyte-macrophage system. A decrease in the content of CD115+ and F4/80+ cells in the spleen was found, which indirectly indicates the migration of monocytes/macrophages after liver resection, which can also influence the course of reparative processes in the liver. |
| first_indexed | 2024-03-08T19:26:03Z |
| format | Article |
| id | doaj.art-ec8fb44eeb9b418fabf7773b44645257 |
| institution | Directory Open Access Journal |
| issn | 2313-0245 2313-0261 |
| language | English |
| last_indexed | 2024-03-08T19:26:03Z |
| publishDate | 2023-12-01 |
| publisher | Peoples’ Friendship University of Russia (RUDN University) |
| record_format | Article |
| series | RUDN Journal of Medicine |
| spelling | doaj.art-ec8fb44eeb9b418fabf7773b446452572023-12-26T09:41:06ZengPeoples’ Friendship University of Russia (RUDN University)RUDN Journal of Medicine2313-02452313-02612023-12-0127444144810.22363/2313-0245-2023-27-4-441-44821131Macrophage population state and proliferative activity of spleen cells under liver regeneration conditionsAiaz T. Mamedov0https://orcid.org/0009-0002-3218-250XElena A. Gantsova1https://orcid.org/0000-0003-4925-8005Viktoria V. Kiseleva2https://orcid.org/0000-0002-3001-4820Anastasia V. Lokhonina3https://orcid.org/0000-0001-8077-2307Andrey V. Makarov4https://orcid.org/0000-0003-2133-2293Svetlana A. Turygina5https://orcid.org/0009-0003-4242-4397Irina A. Bicherova6https://orcid.org/0009-0005-8538-3614Irina V. Arutyunyan7https://orcid.org/0000-0002-4344-8943Polina A. Vishnyakova8https://orcid.org/0000-0001-8650-8240Andrey V. Elchaninov9https://orcid.org/0000-0002-2392-4439Timur Kh. Fatkhudinov10https://orcid.org/0000-0002-6498-5764Pirogov Russian National Research Medical UniversityAvtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. KulakovAvtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»Pirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityAvtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. KulakovPirogov Russian National Research Medical UniversityAvtsyn Research Institute of Human Morphology of Federal state budgetary scientific institution «Petrovsky National Research Centre of Surgery»Relevance. Currently, the participation of immune system cells in the regulation of reparative processes is attracting more and more attention of researchers. There is an anatomical connection between the liver and spleen by means of portal vein. Thus, cytokines and other biologically active substances can enter the liver from the spleen through the portal vein, as well as cells can migrate to the liver. However, the specific mechanisms of mutual influence of the mentioned organs, including in reparative processes, remain poorly studied. The aim of our work was to study the state of spleen monocyte-macrophage population after liver resection, as well as the proliferative activity of spleen cells during liver regeneration . Materials and Methods . The model of liver regeneration after 70 % resection in mouse was reproduced in this work. The animals were taken out of the experiment after 1, 3 and 7 days. The marker of cell proliferation Ki67 was immunohistochemically detected, the state of spleen monocyte-macrophage population was evaluated by markers CD68, CD115, CD206, F4/80 by methods of immunohistochemistry and flow cytometry. Results and Discussion . The liver regeneration had a pronounced effect on the cytoarchitectonics of the spleen. In 1 day after liver resection in the spleen there was observed a decrease in the share of Ki67+cells, according to the flow cytometry data there was a decrease in the number of CD115+cells, in 3 and 7 days there was a decrease in the number of F4/80+ macrophages. Conclusion . Liver resection causes changes in the state of cell populations of the spleen as well. First of all, to the decrease in the activity of proliferative processes in it, as well as to the changes in the state of the monocyte-macrophage system. A decrease in the content of CD115+ and F4/80+ cells in the spleen was found, which indirectly indicates the migration of monocytes/macrophages after liver resection, which can also influence the course of reparative processes in the liver.https://journals.rudn.ru/medicine/article/viewFile/37170/22870regenerationliverresectionspleenmacrophages |
| spellingShingle | Aiaz T. Mamedov Elena A. Gantsova Viktoria V. Kiseleva Anastasia V. Lokhonina Andrey V. Makarov Svetlana A. Turygina Irina A. Bicherova Irina V. Arutyunyan Polina A. Vishnyakova Andrey V. Elchaninov Timur Kh. Fatkhudinov Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions RUDN Journal of Medicine regeneration liver resection spleen macrophages |
| title | Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions |
| title_full | Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions |
| title_fullStr | Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions |
| title_full_unstemmed | Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions |
| title_short | Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions |
| title_sort | macrophage population state and proliferative activity of spleen cells under liver regeneration conditions |
| topic | regeneration liver resection spleen macrophages |
| url | https://journals.rudn.ru/medicine/article/viewFile/37170/22870 |
| work_keys_str_mv | AT aiaztmamedov macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT elenaagantsova macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT viktoriavkiseleva macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT anastasiavlokhonina macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT andreyvmakarov macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT svetlanaaturygina macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT irinaabicherova macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT irinavarutyunyan macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT polinaavishnyakova macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT andreyvelchaninov macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions AT timurkhfatkhudinov macrophagepopulationstateandproliferativeactivityofspleencellsunderliverregenerationconditions |