Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds
Cartilage stem/progenitor cells (CSPCs) are cartilage-specific, multipotent progenitor cells residing in articular cartilage. In this study, we investigated the characteristics and potential of human CSPCs combined with poly(lactic-co-glycolic acid) (PLGA) scaffolds to induce osteochondral regenerat...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2073-4409/10/12/3536 |
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| author | Hsueh-Chun Wang Tzu-Hsiang Lin Che-Chia Hsu Ming-Long Yeh |
| author_facet | Hsueh-Chun Wang Tzu-Hsiang Lin Che-Chia Hsu Ming-Long Yeh |
| author_sort | Hsueh-Chun Wang |
| collection | DOAJ |
| description | Cartilage stem/progenitor cells (CSPCs) are cartilage-specific, multipotent progenitor cells residing in articular cartilage. In this study, we investigated the characteristics and potential of human CSPCs combined with poly(lactic-co-glycolic acid) (PLGA) scaffolds to induce osteochondral regeneration in rabbit knees. We isolated CSPCs from human adult articular cartilage undergoing total knee replacement (TKR) surgery. We characterized CSPCs and compared them with infrapatellar fat pad-derived stem cells (IFPs) in a colony formation assay and by multilineage differentiation analysis in vitro. We further evaluated the osteochondral regeneration of the CSPC-loaded PLGA scaffold during osteochondral defect repair in rabbits. The characteristics of CSPCs were similar to those of mesenchymal stem cells (MSCs) and exhibited chondrogenic and osteogenic phenotypes without chemical induction. For in vivo analysis, CSPC-loaded PLGA scaffolds produced a hyaline-like cartilaginous tissue, which showed good integration with the host tissue and subchondral bone. Furthermore, CSPCs migrated in response to injury to promote subchondral bone regeneration. Overall, we demonstrated that CSPCs can promote osteochondral regeneration. A monophasic approach of using diseased CSPCs combined with a PLGA scaffold may be beneficial for repairing complex tissues, such as osteochondral tissue. |
| first_indexed | 2024-03-10T04:26:19Z |
| format | Article |
| id | doaj.art-ec9aabc12b1741a29d243a704cea5f4b |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T04:26:19Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-ec9aabc12b1741a29d243a704cea5f4b2023-11-23T07:39:07ZengMDPI AGCells2073-44092021-12-011012353610.3390/cells10123536Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous ScaffoldsHsueh-Chun Wang0Tzu-Hsiang Lin1Che-Chia Hsu2Ming-Long Yeh3Department of Biomedical Engineering, National Cheng Kung University, Tainan City 70101, TaiwanDepartment of Biomedical Engineering, National Cheng Kung University, Tainan City 70101, TaiwanDepartment of Orthopedic Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan City 70403, TaiwanDepartment of Biomedical Engineering, National Cheng Kung University, Tainan City 70101, TaiwanCartilage stem/progenitor cells (CSPCs) are cartilage-specific, multipotent progenitor cells residing in articular cartilage. In this study, we investigated the characteristics and potential of human CSPCs combined with poly(lactic-co-glycolic acid) (PLGA) scaffolds to induce osteochondral regeneration in rabbit knees. We isolated CSPCs from human adult articular cartilage undergoing total knee replacement (TKR) surgery. We characterized CSPCs and compared them with infrapatellar fat pad-derived stem cells (IFPs) in a colony formation assay and by multilineage differentiation analysis in vitro. We further evaluated the osteochondral regeneration of the CSPC-loaded PLGA scaffold during osteochondral defect repair in rabbits. The characteristics of CSPCs were similar to those of mesenchymal stem cells (MSCs) and exhibited chondrogenic and osteogenic phenotypes without chemical induction. For in vivo analysis, CSPC-loaded PLGA scaffolds produced a hyaline-like cartilaginous tissue, which showed good integration with the host tissue and subchondral bone. Furthermore, CSPCs migrated in response to injury to promote subchondral bone regeneration. Overall, we demonstrated that CSPCs can promote osteochondral regeneration. A monophasic approach of using diseased CSPCs combined with a PLGA scaffold may be beneficial for repairing complex tissues, such as osteochondral tissue.https://www.mdpi.com/2073-4409/10/12/3536osteochondral tissue engineeringcartilage stem/progenitor cellpoly (lactic-co-glycolic acid) scaffoldmigrationmonophasic approach |
| spellingShingle | Hsueh-Chun Wang Tzu-Hsiang Lin Che-Chia Hsu Ming-Long Yeh Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds Cells osteochondral tissue engineering cartilage stem/progenitor cell poly (lactic-co-glycolic acid) scaffold migration monophasic approach |
| title | Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds |
| title_full | Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds |
| title_fullStr | Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds |
| title_full_unstemmed | Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds |
| title_short | Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds |
| title_sort | restoring osteochondral defects through the differentiation potential of cartilage stem progenitor cells cultivated on porous scaffolds |
| topic | osteochondral tissue engineering cartilage stem/progenitor cell poly (lactic-co-glycolic acid) scaffold migration monophasic approach |
| url | https://www.mdpi.com/2073-4409/10/12/3536 |
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