Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division.
BACKGROUND: In eukaryotes, the spindle assembly checkpoint (SAC) ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the microtubules of the spindle. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the mechanism underlying this surveillance mechanism in p...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2728542?pdf=render |
| _version_ | 1818136439784734720 |
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| author | Marie-Cécile Caillaud Laetitia Paganelli Philippe Lecomte Laurent Deslandes Michaël Quentin Yann Pecrix Manuel Le Bris Nicolas Marfaing Pierre Abad Bruno Favery |
| author_facet | Marie-Cécile Caillaud Laetitia Paganelli Philippe Lecomte Laurent Deslandes Michaël Quentin Yann Pecrix Manuel Le Bris Nicolas Marfaing Pierre Abad Bruno Favery |
| author_sort | Marie-Cécile Caillaud |
| collection | DOAJ |
| description | BACKGROUND: In eukaryotes, the spindle assembly checkpoint (SAC) ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the microtubules of the spindle. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the mechanism underlying this surveillance mechanism in plants, by characterising the orthogolous SAC proteins BUBR1, BUB3 and MAD2 from Arabidopsis. We showed that the cell cycle-regulated BUBR1, BUB3.1 and MAD2 proteins interacted physically with each other. Furthermore, BUBR1 and MAD2 interacted specifically at chromocenters. Following SAC activation by global defects in spindle assembly, these three interacting partners localised to unattached kinetochores. In addition, in cases of 'wait anaphase', plant SAC proteins were associated with both kinetochores and kinetochore microtubules. Unexpectedly, BUB3.1 was also found in the phragmoplast midline during the final step of cell division in plants. CONCLUSIONS/SIGNIFICANCE: We conclude that plant BUBR1, BUB3.1 and MAD2 proteins may have the SAC protein functions conserved from yeast to humans. The association of BUB3.1 with both unattached kinetochore and phragmoplast suggests that in plant, BUB3.1 may have other roles beyond the spindle assembly checkpoint itself. Finally, this study of the SAC dynamics pinpoints uncharacterised roles of this surveillance mechanism in plant cell division. |
| first_indexed | 2024-12-11T09:40:26Z |
| format | Article |
| id | doaj.art-ec9b7ee0092240b89f65074df8f7c8f3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T09:40:26Z |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ec9b7ee0092240b89f65074df8f7c8f32022-12-22T01:12:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0148e675710.1371/journal.pone.0006757Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division.Marie-Cécile CaillaudLaetitia PaganelliPhilippe LecomteLaurent DeslandesMichaël QuentinYann PecrixManuel Le BrisNicolas MarfaingPierre AbadBruno FaveryBACKGROUND: In eukaryotes, the spindle assembly checkpoint (SAC) ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the microtubules of the spindle. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the mechanism underlying this surveillance mechanism in plants, by characterising the orthogolous SAC proteins BUBR1, BUB3 and MAD2 from Arabidopsis. We showed that the cell cycle-regulated BUBR1, BUB3.1 and MAD2 proteins interacted physically with each other. Furthermore, BUBR1 and MAD2 interacted specifically at chromocenters. Following SAC activation by global defects in spindle assembly, these three interacting partners localised to unattached kinetochores. In addition, in cases of 'wait anaphase', plant SAC proteins were associated with both kinetochores and kinetochore microtubules. Unexpectedly, BUB3.1 was also found in the phragmoplast midline during the final step of cell division in plants. CONCLUSIONS/SIGNIFICANCE: We conclude that plant BUBR1, BUB3.1 and MAD2 proteins may have the SAC protein functions conserved from yeast to humans. The association of BUB3.1 with both unattached kinetochore and phragmoplast suggests that in plant, BUB3.1 may have other roles beyond the spindle assembly checkpoint itself. Finally, this study of the SAC dynamics pinpoints uncharacterised roles of this surveillance mechanism in plant cell division.http://europepmc.org/articles/PMC2728542?pdf=render |
| spellingShingle | Marie-Cécile Caillaud Laetitia Paganelli Philippe Lecomte Laurent Deslandes Michaël Quentin Yann Pecrix Manuel Le Bris Nicolas Marfaing Pierre Abad Bruno Favery Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. PLoS ONE |
| title | Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. |
| title_full | Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. |
| title_fullStr | Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. |
| title_full_unstemmed | Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. |
| title_short | Spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division. |
| title_sort | spindle assembly checkpoint protein dynamics reveal conserved and unsuspected roles in plant cell division |
| url | http://europepmc.org/articles/PMC2728542?pdf=render |
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