Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, Iran
Diarrheagenic <i>E. coli</i> (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of...
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MDPI AG
2022-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/7/960 |
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| author | Arghavan Madani Zahra Esfandiari Parisa Shoaei Behrooz Ataei |
| author_facet | Arghavan Madani Zahra Esfandiari Parisa Shoaei Behrooz Ataei |
| author_sort | Arghavan Madani |
| collection | DOAJ |
| description | Diarrheagenic <i>E. coli</i> (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of 200 samples, including traditional and pasteurized dairy products, were analyzed. In 200 samples, 54 <i>E. coli</i> isolates, including (48/110) and (6/90) positive samples of traditional and pasteurized dairy products, were detected. Furthermore, pathogenic strains were isolated from 30% of traditional dairy products and 5.55% of pasteurized dairy products. Most isolates were classified as enteropathogenic <i>E. coli</i> (EPEC). Moreover, antibiotic resistance was evaluated using the disk diffusion method for pathogenic <i>E. coli</i>. Overall, 73.68% of contaminated samples by pathogenic strains were resistant to at least one antibiotic. The highest resistance was observed against streptomycin (57.9%), followed by tetracycline (50%). Additionally, all isolates were sensitive to amikacin. For evaluating biofilm formation, the violet crystal assay was applied on a polystyrene microplate well for pathogenic isolates. In total, 68.42% of isolates were able to form biofilms. The presence of <i>E. coli</i> in dairy products indicates potential health risks for Iranian consumers. Serious measures are needed to control and prevent the spread of this pathogen. |
| first_indexed | 2024-03-09T11:50:59Z |
| format | Article |
| id | doaj.art-ec9ea9f4359f4245996b17f761c70380 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T11:50:59Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ec9ea9f4359f4245996b17f761c703802023-11-30T23:15:03ZengMDPI AGFoods2304-81582022-03-0111796010.3390/foods11070960Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, IranArghavan Madani0Zahra Esfandiari1Parisa Shoaei2Behrooz Ataei3Department of Food Science and Technology, Food Security Research Center, School of Nutrition and Food Science, P. O. Box: 81746-73461, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Food Science and Technology, Food Security Research Center, School of Nutrition and Food Science, P. O. Box: 81746-73461, Isfahan University of Medical Sciences, Isfahan, IranNosocomial Infection Research Center, P. O. Box: 81746-73461, Isfahan University of Medical Sciences, Isfahan, IranNosocomial Infection Research Center, P. O. Box: 81746-73461, Isfahan University of Medical Sciences, Isfahan, IranDiarrheagenic <i>E. coli</i> (DEC) strains are important causes of gastrointestinal diseases worldwide, especially in developing countries. This study aimed to investigate the presence, antibiotic resistance, and potential biofilm formation in dairy products in Isfahan, Iran. A total of 200 samples, including traditional and pasteurized dairy products, were analyzed. In 200 samples, 54 <i>E. coli</i> isolates, including (48/110) and (6/90) positive samples of traditional and pasteurized dairy products, were detected. Furthermore, pathogenic strains were isolated from 30% of traditional dairy products and 5.55% of pasteurized dairy products. Most isolates were classified as enteropathogenic <i>E. coli</i> (EPEC). Moreover, antibiotic resistance was evaluated using the disk diffusion method for pathogenic <i>E. coli</i>. Overall, 73.68% of contaminated samples by pathogenic strains were resistant to at least one antibiotic. The highest resistance was observed against streptomycin (57.9%), followed by tetracycline (50%). Additionally, all isolates were sensitive to amikacin. For evaluating biofilm formation, the violet crystal assay was applied on a polystyrene microplate well for pathogenic isolates. In total, 68.42% of isolates were able to form biofilms. The presence of <i>E. coli</i> in dairy products indicates potential health risks for Iranian consumers. Serious measures are needed to control and prevent the spread of this pathogen.https://www.mdpi.com/2304-8158/11/7/960diarrheagenic <i>E. coli</i>antibiotic resistancebiofilmmilkdairy productsIran |
| spellingShingle | Arghavan Madani Zahra Esfandiari Parisa Shoaei Behrooz Ataei Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, Iran Foods diarrheagenic <i>E. coli</i> antibiotic resistance biofilm milk dairy products Iran |
| title | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, Iran |
| title_full | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, Iran |
| title_fullStr | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, Iran |
| title_full_unstemmed | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, Iran |
| title_short | Evaluation of Virulence Factors, Antibiotic Resistance, and Biofilm Formation of <i>Escherichia coli</i> Isolated from Milk and Dairy Products in Isfahan, Iran |
| title_sort | evaluation of virulence factors antibiotic resistance and biofilm formation of i escherichia coli i isolated from milk and dairy products in isfahan iran |
| topic | diarrheagenic <i>E. coli</i> antibiotic resistance biofilm milk dairy products Iran |
| url | https://www.mdpi.com/2304-8158/11/7/960 |
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