First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved]
Background: Fusarium rot is a newly introduced, devastating disease of citrus fruits. The current investigation was undertaken to characterize the microbes responsible for fruit rot in Citrus reticulata. Methods: Pathogens were isolated from infected citrus fruits using morphological and molecular a...
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F1000 Research Ltd
2023-02-01
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| Online Access: | https://f1000research.com/articles/9-1212/v2 |
| _version_ | 1797893882714783744 |
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| author | Mohammed Faruk Hasan Biswanath Sikdar Mohammed Asadul Islam |
| author_facet | Mohammed Faruk Hasan Biswanath Sikdar Mohammed Asadul Islam |
| author_sort | Mohammed Faruk Hasan |
| collection | DOAJ |
| description | Background: Fusarium rot is a newly introduced, devastating disease of citrus fruits. The current investigation was undertaken to characterize the microbes responsible for fruit rot in Citrus reticulata. Methods: Pathogens were isolated from infected citrus fruits using morphological and molecular approaches. For confirmation of the isolated fungi, polymerase chain reaction (PCR) amplification and internal transcribed spacer gene sequencing techniques were used. Results: The isolated fungus was grown on potato dextrose agar for three days and it produced clamydospores, hyphae and macroconidia. PCR amplification of isolated fungal DNA gave a 650 bp product. The sequence obtained from isolated fungi had 99.42% similarity with the reference Fusarium concentricum sequence in NCBI GenBank. The obtained sequence was deposited in GenBank (Accession No. MT856371). Two isolates showed virulence capability on fresh guava, sweet orange and tomato fruits, which confirmed species identification and Koch’s postulates. Artificially inoculated fungal species grown on tested fruits showed typical Fusarium species symptoms. Conclusions: Outcomes of the present study are beneficial for the detection of this detrimental disease in postharvest Citrus reticulata fruits. Further research is needed for the control of this economically important disease. This is the first study of fruit rot in Citrus reticulata caused by Fusarium in Bangladesh. |
| first_indexed | 2024-04-10T07:00:00Z |
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| id | doaj.art-eca3dac6cb1e4c059398b524201266b4 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-04-10T07:00:00Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-eca3dac6cb1e4c059398b524201266b42023-02-28T01:00:00ZengF1000 Research LtdF1000Research2046-14022023-02-019142811First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved]Mohammed Faruk Hasan0https://orcid.org/0000-0003-2746-5994Biswanath Sikdar1Mohammed Asadul Islam2Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Rajshahi, Rajshahi, 6205, BangladeshProfessor Joarder DNA & Chromosome Research Lab., Dept. of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, 6205, BangladeshProfessor Joarder DNA & Chromosome Research Lab., Dept. of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, 6205, BangladeshBackground: Fusarium rot is a newly introduced, devastating disease of citrus fruits. The current investigation was undertaken to characterize the microbes responsible for fruit rot in Citrus reticulata. Methods: Pathogens were isolated from infected citrus fruits using morphological and molecular approaches. For confirmation of the isolated fungi, polymerase chain reaction (PCR) amplification and internal transcribed spacer gene sequencing techniques were used. Results: The isolated fungus was grown on potato dextrose agar for three days and it produced clamydospores, hyphae and macroconidia. PCR amplification of isolated fungal DNA gave a 650 bp product. The sequence obtained from isolated fungi had 99.42% similarity with the reference Fusarium concentricum sequence in NCBI GenBank. The obtained sequence was deposited in GenBank (Accession No. MT856371). Two isolates showed virulence capability on fresh guava, sweet orange and tomato fruits, which confirmed species identification and Koch’s postulates. Artificially inoculated fungal species grown on tested fruits showed typical Fusarium species symptoms. Conclusions: Outcomes of the present study are beneficial for the detection of this detrimental disease in postharvest Citrus reticulata fruits. Further research is needed for the control of this economically important disease. This is the first study of fruit rot in Citrus reticulata caused by Fusarium in Bangladesh.https://f1000research.com/articles/9-1212/v2Citrus reticulata Fruit Rot Fusarium sp. PCR ITS rRNA geneeng |
| spellingShingle | Mohammed Faruk Hasan Biswanath Sikdar Mohammed Asadul Islam First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved] F1000Research Citrus reticulata Fruit Rot Fusarium sp. PCR ITS rRNA gene eng |
| title | First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved] |
| title_full | First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved] |
| title_fullStr | First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved] |
| title_full_unstemmed | First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved] |
| title_short | First report on molecular identification of Fusarium species causing fruit rot of mandarin (Citrus reticulata) in Bangladesh [version 2; peer review: 2 approved] |
| title_sort | first report on molecular identification of fusarium species causing fruit rot of mandarin citrus reticulata in bangladesh version 2 peer review 2 approved |
| topic | Citrus reticulata Fruit Rot Fusarium sp. PCR ITS rRNA gene eng |
| url | https://f1000research.com/articles/9-1212/v2 |
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