Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
In cell therapy, transplanting an appropriate number of cells to the target site is crucial. One way to achieve this is to transplant cell sheets. Transplantation of cell sheets has already been utilized for various diseases in clinical practice. However, reducing the cost of cell sheet utilization...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580821002636 |
| _version_ | 1819177716561215488 |
|---|---|
| author | Koji Ueno Soichi Ike Naohiro Yamamoto Yutaro Matsuno Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Bungo Shirasawa Kimikazu Hamano |
| author_facet | Koji Ueno Soichi Ike Naohiro Yamamoto Yutaro Matsuno Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Bungo Shirasawa Kimikazu Hamano |
| author_sort | Koji Ueno |
| collection | DOAJ |
| description | In cell therapy, transplanting an appropriate number of cells to the target site is crucial. One way to achieve this is to transplant cell sheets. Transplantation of cell sheets has already been utilized for various diseases in clinical practice. However, reducing the cost of cell sheet utilization is essential so as to facilitate the spread of regenerative medicine. Several ways to reduce costs are available, one of which is the use of allogenic cells. Another alternative is the use of cell sheets, which necessitates the development of methods for freezing cell sheets. This is the first study to report the use of a 3D Freezer for freezing cells. 3D Freezers have been used in the field of food processing and technology for a long time. The 3D Freezer freezes objects using cold air at a uniform temperature from all directions. In this study, we analyzed the cooling speed of human fibroblast sheets in 11 cell preservation solutions using a 3D Freezer and a Program Freezer. The cooling speed was −2 °C per min in the 3D Freezer. Supercooling in 10 cell preservation solutions was lower in the 3D Freezer than in the Program Freezer. Cell viability after freeze–thaw of the cell sheets using 3D Freezer was more than 70% in five cell preservation solutions. The levels of hepatocyte growth factor and transforming growth factor-β1 were the same not only in the fibroblast sheets frozen using the five cell preservation solutions but also in the non-frozen fibroblast sheets. These results suggest that the 3D Freezer can freeze implantable cell sheets immediately after thawing. |
| first_indexed | 2024-12-22T21:31:05Z |
| format | Article |
| id | doaj.art-eca82acbfb9042ee80bc703f33e41577 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-22T21:31:05Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-eca82acbfb9042ee80bc703f33e415772022-12-21T18:11:55ZengElsevierBiochemistry and Biophysics Reports2405-58082021-12-0128101169Freezing of cell sheets using a 3D freezer produces high cell viability after thawingKoji Ueno0Soichi Ike1Naohiro Yamamoto2Yutaro Matsuno3Hiroshi Kurazumi4Ryo Suzuki5Shunsaku Katsura6Bungo Shirasawa7Kimikazu Hamano8Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, Japan; Corresponding author. Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi, 755-8505, Japan.Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, JapanDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, JapanDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, JapanDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, JapanDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, JapanDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, Japan; Department of Medical Education, Yamaguchi University Graduate School of Medicine, Ube, JapanDepartment of Medical Education, Yamaguchi University Graduate School of Medicine, Ube, JapanDepartment of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Ube, JapanIn cell therapy, transplanting an appropriate number of cells to the target site is crucial. One way to achieve this is to transplant cell sheets. Transplantation of cell sheets has already been utilized for various diseases in clinical practice. However, reducing the cost of cell sheet utilization is essential so as to facilitate the spread of regenerative medicine. Several ways to reduce costs are available, one of which is the use of allogenic cells. Another alternative is the use of cell sheets, which necessitates the development of methods for freezing cell sheets. This is the first study to report the use of a 3D Freezer for freezing cells. 3D Freezers have been used in the field of food processing and technology for a long time. The 3D Freezer freezes objects using cold air at a uniform temperature from all directions. In this study, we analyzed the cooling speed of human fibroblast sheets in 11 cell preservation solutions using a 3D Freezer and a Program Freezer. The cooling speed was −2 °C per min in the 3D Freezer. Supercooling in 10 cell preservation solutions was lower in the 3D Freezer than in the Program Freezer. Cell viability after freeze–thaw of the cell sheets using 3D Freezer was more than 70% in five cell preservation solutions. The levels of hepatocyte growth factor and transforming growth factor-β1 were the same not only in the fibroblast sheets frozen using the five cell preservation solutions but also in the non-frozen fibroblast sheets. These results suggest that the 3D Freezer can freeze implantable cell sheets immediately after thawing.http://www.sciencedirect.com/science/article/pii/S2405580821002636Cell sheetFreezing3D freezerSurvivalRegenerative medicineTemperature |
| spellingShingle | Koji Ueno Soichi Ike Naohiro Yamamoto Yutaro Matsuno Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Bungo Shirasawa Kimikazu Hamano Freezing of cell sheets using a 3D freezer produces high cell viability after thawing Biochemistry and Biophysics Reports Cell sheet Freezing 3D freezer Survival Regenerative medicine Temperature |
| title | Freezing of cell sheets using a 3D freezer produces high cell viability after thawing |
| title_full | Freezing of cell sheets using a 3D freezer produces high cell viability after thawing |
| title_fullStr | Freezing of cell sheets using a 3D freezer produces high cell viability after thawing |
| title_full_unstemmed | Freezing of cell sheets using a 3D freezer produces high cell viability after thawing |
| title_short | Freezing of cell sheets using a 3D freezer produces high cell viability after thawing |
| title_sort | freezing of cell sheets using a 3d freezer produces high cell viability after thawing |
| topic | Cell sheet Freezing 3D freezer Survival Regenerative medicine Temperature |
| url | http://www.sciencedirect.com/science/article/pii/S2405580821002636 |
| work_keys_str_mv | AT kojiueno freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT soichiike freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT naohiroyamamoto freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT yutaromatsuno freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT hiroshikurazumi freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT ryosuzuki freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT shunsakukatsura freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT bungoshirasawa freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT kimikazuhamano freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing |