Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.)
Wheat leaf rust, caused by fungal pathogen Puccinia triticina Erikss, annually contributes to production losses as high as 40% in susceptible varieties and remains as one of the most damaging diseases of wheat worldwide. Currently, one of the major challenges of wheat geneticists and breeders is to...
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-02-01
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| Series: | Biomolecular Concepts |
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| Online Access: | https://doi.org/10.1515/bmc-2022-0002 |
| _version_ | 1811236723504447488 |
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| author | Bobrowska Roksana Noweiska Aleksandra Spychała Julia Tomkowiak Agnieszka Nawracała Jerzy Kwiatek Michał T. |
| author_facet | Bobrowska Roksana Noweiska Aleksandra Spychała Julia Tomkowiak Agnieszka Nawracała Jerzy Kwiatek Michał T. |
| author_sort | Bobrowska Roksana |
| collection | DOAJ |
| description | Wheat leaf rust, caused by fungal pathogen Puccinia triticina Erikss, annually contributes to production losses as high as 40% in susceptible varieties and remains as one of the most damaging diseases of wheat worldwide. Currently, one of the major challenges of wheat geneticists and breeders is to accumulate major genes for durability of rust resistance called “slow rusting” genes using marker-assisted selection (MAS). Until now, eight genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr74, Lr75, Lr77, and Lr78) conferring resistance against multiple fungal pathogens have been identified in wheat gene pool and the molecular markers were developed for them. In MAS practice, it is a common problem that cultivars exhibiting desirable marker genotypes may not necessarily have the targeted genes or alleles and vice versa, which is known as “false positives.” The aim of this study was to compare the available four markers: Xwmc44, Xgwm259, Xbarc80, and csLV46G22 markers (not published yet), for the identification of the Lr46/Yr29 loci in 73 genotypes of wheat, which were reported as sources of various “slow rusting” genes, including 60 with confirmed Lr46/Yr29 gene, reported in the literature. This research revealed that csLV46G22 together with Xwmc44 is most suitable for the identification of resistance allele of the Lr46/Yr29 gene; however, there is a need to clone the Lr46/Yr29 loci to identify and verify the allelic variation of the gene and the function. |
| first_indexed | 2024-04-12T12:12:56Z |
| format | Article |
| id | doaj.art-ecab23a9eff54b80bc3b06a713dbaf53 |
| institution | Directory Open Access Journal |
| issn | 1868-503X |
| language | English |
| last_indexed | 2024-04-12T12:12:56Z |
| publishDate | 2022-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Biomolecular Concepts |
| spelling | doaj.art-ecab23a9eff54b80bc3b06a713dbaf532022-12-22T03:33:32ZengDe GruyterBiomolecular Concepts1868-503X2022-02-011311910.1515/bmc-2022-0002Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.)Bobrowska Roksana0Noweiska Aleksandra1Spychała Julia2Tomkowiak Agnieszka3Nawracała Jerzy4Kwiatek Michał T.5Department of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, PolandDepartment of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, PolandDepartment of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, PolandDepartment of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, PolandDepartment of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, PolandDepartment of Genetics and Plant Breeding, Faculty of Agronomy, Horticulture and Bioengineering, Poznań University of Life Sciences, 11 Dojazd Str, 60-632 Poznań, PolandWheat leaf rust, caused by fungal pathogen Puccinia triticina Erikss, annually contributes to production losses as high as 40% in susceptible varieties and remains as one of the most damaging diseases of wheat worldwide. Currently, one of the major challenges of wheat geneticists and breeders is to accumulate major genes for durability of rust resistance called “slow rusting” genes using marker-assisted selection (MAS). Until now, eight genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr74, Lr75, Lr77, and Lr78) conferring resistance against multiple fungal pathogens have been identified in wheat gene pool and the molecular markers were developed for them. In MAS practice, it is a common problem that cultivars exhibiting desirable marker genotypes may not necessarily have the targeted genes or alleles and vice versa, which is known as “false positives.” The aim of this study was to compare the available four markers: Xwmc44, Xgwm259, Xbarc80, and csLV46G22 markers (not published yet), for the identification of the Lr46/Yr29 loci in 73 genotypes of wheat, which were reported as sources of various “slow rusting” genes, including 60 with confirmed Lr46/Yr29 gene, reported in the literature. This research revealed that csLV46G22 together with Xwmc44 is most suitable for the identification of resistance allele of the Lr46/Yr29 gene; however, there is a need to clone the Lr46/Yr29 loci to identify and verify the allelic variation of the gene and the function.https://doi.org/10.1515/bmc-2022-0002cslv46g22molecular markerspuccinia triticinawheatxbarc80xgwm259xwmc44 |
| spellingShingle | Bobrowska Roksana Noweiska Aleksandra Spychała Julia Tomkowiak Agnieszka Nawracała Jerzy Kwiatek Michał T. Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.) Biomolecular Concepts cslv46g22 molecular markers puccinia triticina wheat xbarc80 xgwm259 xwmc44 |
| title | Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.) |
| title_full | Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.) |
| title_fullStr | Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.) |
| title_short | Diagnostic accuracy of genetic markers for identification of the Lr46/Yr29 “slow rusting” locus in wheat (Triticum aestivum L.) |
| title_sort | diagnostic accuracy of genetic markers for identification of the lr46 yr29 slow rusting locus in wheat triticum aestivum l |
| topic | cslv46g22 molecular markers puccinia triticina wheat xbarc80 xgwm259 xwmc44 |
| url | https://doi.org/10.1515/bmc-2022-0002 |
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