Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies
A considerable interest in cancer research is represented by the development of magnetic nanoparticles based on biofunctionalized polymers for controlled-release systems of hydrophobic chemotherapeutic drugs targeted only to the tumor sites, without affecting normal cells. The objective of the paper...
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3467 |
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| author | Vlad Constantin Ursachi Gianina Dodi Alina Gabriela Rusu Cosmin Teodor Mihai Liliana Verestiuc Vera Balan |
| author_facet | Vlad Constantin Ursachi Gianina Dodi Alina Gabriela Rusu Cosmin Teodor Mihai Liliana Verestiuc Vera Balan |
| author_sort | Vlad Constantin Ursachi |
| collection | DOAJ |
| description | A considerable interest in cancer research is represented by the development of magnetic nanoparticles based on biofunctionalized polymers for controlled-release systems of hydrophobic chemotherapeutic drugs targeted only to the tumor sites, without affecting normal cells. The objective of the paper is to present the synthesis and in vitro evaluation of the nanocomposites that include a magnetic core able to direct the systems to the target, a polymeric surface shell that provides stabilization and multi-functionality, a chemotherapeutic agent, Paclitaxel (PTX), and a biotin tumor recognition layer. To our best knowledge, there are no studies concerning development of magnetic nanoparticles obtained by partial oxidation, based on biotinylated <i>N</i>-palmitoyl chitosan loaded with PTX. The structure, external morphology, size distribution, colloidal and magnetic properties analyses confirmed the formation of well-defined crystalline magnetite conjugates, with broad distribution, relatively high saturation magnetization and irregular shape. Even if the ability of the nanoparticles to release the drug in 72 h was demonstrated, further complex in vitro and in vivo studies will be performed in order to validate the magnetic nanoparticles as PTX delivery system. |
| first_indexed | 2024-03-10T10:39:33Z |
| format | Article |
| id | doaj.art-ecab79cf775542288e6c49d6d9045c97 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:39:33Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ecab79cf775542288e6c49d6d9045c972023-11-21T23:07:24ZengMDPI AGMolecules1420-30492021-06-012611346710.3390/molecules26113467Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro StudiesVlad Constantin Ursachi0Gianina Dodi1Alina Gabriela Rusu2Cosmin Teodor Mihai3Liliana Verestiuc4Vera Balan5Faculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy of Iasi, 700115 Iasi, RomaniaAdvanced Centre for Research-Development in Experimental Medicine, Grigore T. Popa University of Medicine and Pharmacy of Iasi, 700115 Iasi, RomaniaNatural Polymers, Bioactive and Biocompatible Materials Laboratory, Petru Poni Institute of Macromolecular Chemistry, 700487 Iasi, RomaniaAdvanced Centre for Research-Development in Experimental Medicine, Grigore T. Popa University of Medicine and Pharmacy of Iasi, 700115 Iasi, RomaniaFaculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy of Iasi, 700115 Iasi, RomaniaFaculty of Medical Bioengineering, Grigore T. Popa University of Medicine and Pharmacy of Iasi, 700115 Iasi, RomaniaA considerable interest in cancer research is represented by the development of magnetic nanoparticles based on biofunctionalized polymers for controlled-release systems of hydrophobic chemotherapeutic drugs targeted only to the tumor sites, without affecting normal cells. The objective of the paper is to present the synthesis and in vitro evaluation of the nanocomposites that include a magnetic core able to direct the systems to the target, a polymeric surface shell that provides stabilization and multi-functionality, a chemotherapeutic agent, Paclitaxel (PTX), and a biotin tumor recognition layer. To our best knowledge, there are no studies concerning development of magnetic nanoparticles obtained by partial oxidation, based on biotinylated <i>N</i>-palmitoyl chitosan loaded with PTX. The structure, external morphology, size distribution, colloidal and magnetic properties analyses confirmed the formation of well-defined crystalline magnetite conjugates, with broad distribution, relatively high saturation magnetization and irregular shape. Even if the ability of the nanoparticles to release the drug in 72 h was demonstrated, further complex in vitro and in vivo studies will be performed in order to validate the magnetic nanoparticles as PTX delivery system.https://www.mdpi.com/1420-3049/26/11/3467magnetic nanoparticleschitosanbiotinpaclitaxeldrug deliveryviability assay |
| spellingShingle | Vlad Constantin Ursachi Gianina Dodi Alina Gabriela Rusu Cosmin Teodor Mihai Liliana Verestiuc Vera Balan Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies Molecules magnetic nanoparticles chitosan biotin paclitaxel drug delivery viability assay |
| title | Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies |
| title_full | Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies |
| title_fullStr | Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies |
| title_full_unstemmed | Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies |
| title_short | Paclitaxel-Loaded Magnetic Nanoparticles Based on Biotinylated <i>N</i>-Palmitoyl Chitosan: Synthesis, Characterization and Preliminary In Vitro Studies |
| title_sort | paclitaxel loaded magnetic nanoparticles based on biotinylated i n i palmitoyl chitosan synthesis characterization and preliminary in vitro studies |
| topic | magnetic nanoparticles chitosan biotin paclitaxel drug delivery viability assay |
| url | https://www.mdpi.com/1420-3049/26/11/3467 |
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